Objective： To evaluate the application of uniform design-high throughput screening technology for screening the active ingredients of traditional Chinese medicine.
Methods： Weperformed uniform design compatibility study on four different saponin monomers ginsenoside Re, Rb1, Rg1, Rg3 and one notoginsenoside R1
each at six concentrations (1×10
-4~1×10
-9 mol·L
-1), and their neuroprotective effects were assessed on the damaged nerve cells deprived of the serum. The obtained optimal combination of the ingredients from the above cell study was subjected to further animal tests for their pharmacological effects against the damages induced by cerebral ischemia reperfusion of mice model; the passive avoidance test, the hypoxia tolerance test were performed and the levels of aging-related enzymes in serum, brain and heart tissue were determined for assessing the effects of these ingredients.
Results： The best combination samples (A11, A12, P5) obtained from cell screening experiment, have higher potency (cell survival ratio﹥90%) than any of five individual saponin monomers and vitamin E (the highest cell survival ratio was 78%) tested at a high concentration of 1×10
-4 mol·L
-1; these combinations significantly improved the learning and memory impairment induced by cerebral ischemia reperfusion injury, enhanced the hypoxia tolerance ability, increased the activity of superoxide dismutase, reduced the content of nitric oxide and maintained stability stable level of glutamic-pyruvic transaminase and lactic dehydrogenase (
P<0.01~0.05).
Conclusion： Uniform design compatibility and the traditional Chinese medicine
compound compatibility have similarities. The best three combination samples obtained from five saponins by high-throughput screening show protective effect on cerebral ischemia reperfusion injury. Uniform design-high throughput screening technology is a reliable technology for massive potency screening of active ingredients of traditional Chinese medicine and compounds.
