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Mechanism of Action of Vaccine Adjuvants and Research Progress ZHANG Xin-yan, SHI Xiao-zi, ZHAO Yong-ming ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 55-.   DOI: 10.3969/j.issn.2095-1396.2023.06.011 Abstract （119）      PDF（pc） （961KB）（282）       Knowledge map    Save Vaccination is currently the most economically effective method for preventing infectious diseases. Adjuvants are substances added to vaccines to enhance antigens immunogenicity. The appropriate addition of adjuvants can better assist vaccines in stimulating the body， achieving efficient and long-lasting immune responses， reducing the production costs of vaccines， and minimizing the dosage of drugs， thus decreasing the toxic side effects of drugs entering the human body. This article provides a comprehensive review of commonly used adjuvants and their immunogenic properties. It aims to serve as a reference for the design and selection of safe and effective vaccine adjuvants. Related Articles | Metrics | Comments（0）

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Observation on the Efficacy and Safety of Baofukang Suppository Combined with Recombinant Human Interferon α-2b in the Treatment of High-Risk HPV Infection CHEN Ying, YOU Ji-wen, LIN Yan, CHEN Yun-xiang ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 18-.   DOI: 10.3969/j.issn.2095-1396.2024.01.003 Abstract （118）      PDF（pc） （1025KB）（66）       Knowledge map    Save Objective：To investigate the efficacy and safety of Baofukang suppository on the basis of recombinant human interferon α-2b in the treatment of high-risk human papillomavirus (HR-HPV) infection. Methods： 80 HR-HPV patients were randomly divided into 2 groups. The control group (40 cases) was treated with recombinant human interferon α-2b vaginal effervescent capsule. The observation group (40 cases) was treated with Baofukang suppository on the basis of recombinant human interferon α-2b vaginal effervescent capsule. Both groups were treated for 3 months. The treatment effects of the two groups were compared.The duration of abnormal secretions, HPV viral load, TCT test results and cervical inflammation scores of the two groups were recorded, and the adverse reactions were observed. Results： Compared with the control group (75.00%), the total effective rate of the observation group (95.00%) was higher (P<0.05). The duration of abnormal secretions in the observation group was (14.47±3.15) d, comparing with the control group (20.12±3.89) d, shows significantly shorter (P<0.05). After treatment, the normal rate of TCT in the two groups was significantly higher than that before treatment (P<0.05 ), and the normal rate of TCT in the observation group was higher than that in the control group(P<0.05). After treatment, the HPV viral load in the observation group decreased comparing with the control group (P<0.05), and the cervical inflammation score decreased (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion： Baofukang suppository based on recombinant human interferon α-2b vaginal effervescent capsule is effective and safe in the treatment of HRHPV infection. Related Articles | Metrics | Comments（0）

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Research Progress of Neuroactive Steroid in the Treatment of Depression ZHANG Ran-ran, WU Zhi-gang ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 49-.   DOI: 10.3969/j.issn.2095-1396.2024.01.009 Abstract （112）      PDF（pc） （1030KB）（73）       Knowledge map    Save Depression is a chronic syndrome with complex etiology. Nearly one-third of patients are not sensitive to current antidepressants. In order to ensure the quality of life of all patients with depression, it is essential to explore new antidepressant treatment methods. Neuroactive steroids are metabolites of steroid hormones play an antidepressant role through multiple pathways and multiple targets. In this paper, the literature on neuroactive steroids in the treatment of depression was summarized to provide scientific basis for neuroactive steroids in the treatment of depression, and to provide new ideas for the development of antidepressant drugs. Related Articles | Metrics | Comments（0）

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Advances in the Regulation of Mitochondrial Dynamics on Tumor Cells NING Kang-hao, YAN Ju-han, YAN Xiao-dong, LI Jia-cheng, LI Xiang-dong ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 57-.   DOI: 10.3969/j.issn.2095-1396.2024.01.010 Abstract （87）      PDF（pc） （994KB）（77）       Knowledge map    Save Mitochondrial dynamics is the dynamic balance process in which mitochondria continuously divide and fuse under physiological or stimulating conditions, thereby regulating the distribution, quantity, size and shape of mitochondria in the cell. In cells, the regulation of mitochondrial dynamics is closely related to the functions of mitochondria, which include providing energy, regulating calcium homeostasis, participating in programmed cell death, cell signal transduction, and cell metabolism. In cancer, mitochondrial dynamic regulation is closely related to the proliferation, migration, treatment and drug resistance of tumor cells. Therefore, mitochondrial dynamic regulation has become a research hotspot in the field of cancer, and has shown great potential in tumor therapy. In this review, we review the key features that mediate mitochondrial dynamic regulation, and discuss the mechanisms involved in tumorigenesis and development and its potential in tumor therapy. Related Articles | Metrics | Comments（0）

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Explore the Mechanism of Quercetin in the Treatment of Alzheimer's  Disease by Network Pharmacology and Molecular Docking WANG Tian-xu, LIU Ci, CUI Yong-yuan, ZHANG Xin, WU Miao-miao, SHEN Li-xia ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 31-.   DOI: 10.3969/j.issn.2095-1396.2024.02.006 Abstract （87）      PDF（pc） （4082KB）（27）       Knowledge map    Save Objective: To investigate the mechanism of quercetin in the treatment of Alzheimer's disease based on network pharmacology and molecular docking. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, PubChem database and Swiss Target Prediction platform were used to predict potential targets of quercetin. Potential targets for Alzheimer's disease were obtained through the Disgenet database. Common targets were obtained by intersection of potential quercetin targets and potential Alzheimer's disease targets through Venn diagram. The common targets were imported into DAVID database for screening at P<0.05, and gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed. The protein-protein interaction (PPI) network was constructed utilizing STRING database. The centiscape 2.2 plug-in of Cytoscape 3.6.0 software was used to analyze the PPI network, and the key target of quercetin in the treatment of Alzheimer’s disease was obtained. The top 5 key targets will be selected as the core targets in order of degree from largest to smallest, and the selected core targets will be subjected to molecular docking with SailVina final software. Results: There were 319 potential targets of quercetin in the three databases, 673 potential targets of Alzheimer's disease, 92 intersection targets, and 23 key targets screened out. The biological processes in GO analysis mainly included positive regulation of gene expression, positive regulation of apoptosis, response to exogenous stimuli, and negative regulation of apoptosis. KEGG pathway analysis mainly concentrated in IL-17, HIF-1, FoxO, TNF, PI3K-Akt, MAPK signaling pathway and so on. The average docking affinity between quercetin and core target molecules was -7.92 kcal·mol-1. Quercetin has good binding activity with the core targets IL-6, AKT, TP53, TNF and IL-1β. Conclusion: The potential targets and signaling pathways of quercetin in the treatment of AD have been found by network pharmacology and molecular docking techniques, which provided theoretical basis for subsequent experimental studies. Related Articles | Metrics | Comments（0）

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Analysis of CYP2C19 Gene Polymorphism in Ischemic Stroke Patients in Zhangjiakou Area, Hebei WANG Peng, XUE Qian ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 23-.   DOI: 10.3969/j.issn.2095-1396.2024.01.004 Abstract （75）      PDF（pc） （1017KB）（53）       Knowledge map    Save Objective：To analyze the distribution and frequency of clopidogrel related gene CYP2C19 polymorphism in patients with acute cerebral infarction in Zhangjiakou area of Hebei province. Methods: 249 patients with acute cerebral infarction admitted to the first affiliated Hospital of Hebei North University from September 2021 to March 2023 were selected. The exfoliated cells of oral mucosa were collected and CYP2C19 genotyping was detected by POCT method. The patients were divided into groups according to sex and age, and the distribution of CYP2C19 genotypes and clopidogrel metabolic types among the groups were compared. Results: Five genotypes were detected: fast metabolic CYP2C19*1/* 1 in 9 6 cases, accounting for 38.60%; CYP2C19*1/*2 in middle metabolic type, accounting for 45.00%, 1 0 cases, accounting for 4.00%; slow metabolic type, 25 cases, accounting for 10.00%, CYP2C19*2/*3, 6 cases, accounting for 2.40%. The frequency distributions of alleles * 1, * 2 and * 3 were 63.05%, 63.05% and 3.21%, respectively. No genotypes of CYP2C19*1/*17, CYP2C19*17/*17, CYP2C19*2/*17,CYP2C19*3/*17 and CYP2C19*3/*3 were detected in this study. There was no significant difference in sex, genotype and metabolic type distribution frequency (P>0.05). There was no significant difference in age, genotype and metabolic type distribution frequency (P>0.05). Conclusion: The CYP2C19 polymorphism of clopidogrel related gene in patients with acute AIS in Zhangjiakou area of Hebei Province is mainly middle metabolic type, and the middle and slow metabolic type accounts for 61.40%. Most people have platelet hyperresponsiveness, so gene detection is carried out before medication to develop a safe, reasonable, effective and economical personalized drug treatment plan. Related Articles | Metrics | Comments（0）

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Research Progress on Mitochondrial Metabolomics and Its Application in Research of Traditional Chinese Medicine HUANG Xing-yue, QIN Xue-mei, LIU Yue-tao ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 59-.   DOI: 10.3969/j.issn.2095-1396.2024.03.008 Abstract （75）      PDF（pc） （919KB）（21）       Knowledge map    Save Mitochondria is a kind of semi-autonomous organelle, which integrates three basic life activities: material metabolism, energy metabolism and genetic variation, and its metabolism is at the core of cell metabolic network. Current metabonomics mostly focuses on the metabolic characteristics of the whole cell, but lacks the specific reflection of mitochondrial metabolism. Therefore, it is necessary to study the metabolism of mitochondria. Traditional Chinese medicine has unique advantages and characteristics in treating diseases based on syndrome differentiation. Mitochondrial metabolism is also one of its important targets. The study of traditional Chinese medicine based on mitochondrial metabonomics is helpful to reveal the mechanism and target of traditional Chinese medicine in energy metabolism network from the perspective of mitochondrial metabolism. At present, there are many problems and difficulties in mitochondrial metabonomics research, such as the extraction, separation and evaluation of mitochondria, metabolites and so on. This paper summarizes the present situation of mitochondrial metabonomics and its research progress in the field of traditional Chinese medicine, which provides technical support and new research direction for the scientific research of traditional Chinese medicine. Related Articles | Metrics | Comments（0）

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Research Progress of Curcumin Nano-Preparations YUAN Xin-ru, ZHANG Dan-shen ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 54-.   DOI: 10.3969/j.issn.2095-1396.2024.02.009 Abstract （74）      PDF（pc） （1011KB）（26）       Knowledge map    Save Curcumin (Cur) is a diketone compound extracted from the rhizomes of the genus turmeric, which has a wide range of pharmacological activities such as antioxidant, anti-inflammatory, anti-tumor, and neuroprotection, but has low solubility, short half-life, low bioavailability, difficult to penetrate the blood-brain barrier and other shortcomings limit its clinical application. In recent years, it has been prepared into liposomes, solid dispersions, polymer nanoparticles, polymer micelles, nanosuspensions, etc., which significantly improved the solubility, bioavailability and targeting of Cur, and improved drug activity. This article reviewed the nano-preparations of Cur, aiming to provide references for its further research and development. Related Articles | Metrics | Comments（0）

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Effects of Sulpiride Tablets Combined with Lurasidone Tablets on Mental Symptoms, Cognitive Function and Serum Prolactin Levels in Patients with Schizophrenia HUANG He, NI Sheng-mei, WU Yu-feng ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 28-.   DOI: 10.3969/j.issn.2095-1396.2024.01.005 Abstract （70）      PDF（pc） （1028KB）（36）       Knowledge map    Save Objective：To investigate the therapeutic effect of sulpiride tablets combined with lurasidone tablets on schizophrenia. Methods: 80 patients with schizophrenia were randomly divided into control group (n=40) and observation group (n=40), they were treated with sulpiride tablets and sulpiride tablets combined with lurasidone tablets respectively. Both groups were treated for 8 weeks. The therapeutic effects of the two groups were evaluated. The changes of mental symptoms, cognitive function [Repeatable Battery for the Assessment of Neuropsychological Status (RBANS)], social function [Personal and Social Performance Scale (PSP)] and serum prolactin (PRL) levels before and after treatment were compared between the two groups, and the adverse reactions were counted. Results: Comparing with the control group (75.00%), the total effective rate of treatment in the observation group (95.00%) was higher(P<0.05). After treatment, the mental symptom score of the observation group was lower, and the RBANS score and PSP score were significantly higher comparing with the control group(P<0.05). After treatment, the serum PRL of the two groups increased significantly(P<0.05), and the difference between the two groups shows no significant (P>0.05). Compared with the control group, there was no significant increase in the incidence of adverse reactions in the observation group (P>0.05). Conclusion: On the basis of sulpiride tablets, the combination of lurasidone tablets in patients with schizophrenia can improve the efficacy and cognitive function of patients, and has no significant effect on serum PRL, with good safety. Related Articles | Metrics | Comments（0）

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Exploring the Mechanism of Ganmai Dazao Decoction in Treating Cardiac Neurosis Based on Network Pharmacology and Molecular Docking HAO Sheng-jie, DU Yu-xin, LU Shang-kai, HUANG Shi-jie, WANG Hui, WANG Bing-jie, ZHAO Bo, LI Meng-wei, LI Qian ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 36-.   DOI: 10.3969/j.issn.2095-1396.2024.03.005 Abstract （70）      PDF（pc） （4417KB）（21）       Knowledge map    Save Objective：To investigate the mechanism of action of Ganmai Dazao Tang in the treatment of cardiac neurosis based on network pharmacology combined with molecular docking. Methods: We searched the Chinese Medicine System Pharmacology (TCMSP) database to obtain the target information of active ingredients, and mined the GeneCards database to construct disease targets. The STRING (https://string-db.org/) database was used to construct protein-protein interaction (PPI) network models, which were visualized and analyzed with Cytoscape 3.8.2. The online analysis platform DAVID v6.8 was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis and gene ontology (GO) enrichment analysis on the key targets. KEGG signaling pathway and GO enrichment analysis were mapped using R package. Molecular docking was accomplished using AutoDock Vina, Pymol 2.0.1, and Openbable 3.1.1. Results: A total of 107 active ingredients including quercetin, lignans, kaempferol, naringenin, isorhamnetin and other biologically important components acted on 203 potential targets for disease drug therapy including STAT3, JUN, TNF, TPT53, AKT1, EGFR. And it was widely enriched in a series of signaling pathways of TNF, HIF-1 and Toll-like receptor, and the core active ingredients were also tightly bound to the core targets. Conclusion: Flavonoids, triterpenoids and phenols in Ganmai Dazao Tang can stably bind to the disease targets of cardiac neurosis, which further confirmed that the formula treats functional disorders, dirty agitation, depression and anxiety through multi-components, multitargets and multi-pathways, which provides scientific basis and reference for the treatment of cardiac neurosis by traditional Chinese medicine. Related Articles | Metrics | Comments（0）

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Efficacy of Ornidazole Injection Combined with Latamoxef Sodium in the Treatment of Pelvic Inflammatory Disease and Its Effect on Serum Inflammatory Factors HU Xiu-ping, CUI Xiao-jie, YANG Fang, HUANG Dai-wei ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 38-.   DOI: 10.3969/j.issn.2095-1396.2024.01.007 Abstract （64）      PDF（pc） （1108KB）（49）       Knowledge map    Save Objective: To explore the efficacy of ornidazole injection combined with latamoxef sodium in the treatment of pelvic inflammatory disease and its effect on serum inflammatory factors. Methods: A total of 97 patients with pelvic inflammatory disease admitted to Xiamen Fifth Hospital from June 2020 to May 2023 were randomly (random number table method) divided into control group (n=49) and observation group (n=48). The control group was treated with intravenous drip of latamoxef sodium, and the observation group was treated with intravenous drip of ornidazole + latamoxef sodium. The clinical efficacy, clinical symptom scores, pelvic effusion, inflammatory mass diameter and serum inflammatory factors [C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT)] levels before and after treatment were compared between the two groups. Results: The total effective rate of the observation group was higher than that of the control group (93.75% vs 77.55%, P<0.05). Compared with the control group, the clinical symptom score and serum CRP, IL-6, PCT levels were lower in the observation group after treatment, and the pelvic effusion volume and inflammatory mass diameter were smaller (P<0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (8.33% vs 6.12%, P>0.05). Conclusion: The combined use of ornidazole injection can significantly improve the efficacy of latamoxef sodium alone in the treatment of pelvic inflammatory disease, improve clinical symptoms and signs, and reduce the level of serum inflammatory factors. Related Articles | Metrics | Comments（0）

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Mechanism of Action of Commelina communis L. in the Treatment of Febrile Seizures Based on Network Pharmacology and Molecular Docking Technology ZHAO Dong-li, LI Zi-rou, YAN Jia-hao, WU Wei, CUI Xiao-yan, YU Yong-zhou ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 1-.   DOI: 10.3969/j.issn.2095-1396.2024.01.001 Abstract （62）      PDF（pc） （7623KB）（47）       Knowledge map    Save Objective：To explore the mechanism of Commelina communis L. in the treatment of febrile seizures based on network pharmacology and molecular docking technology.Methods： The effective components of Commelina communis L. and their related targets were extracted by searching the Chinese Medicine System Pharmacology (TCMSP) database and BATMAN-TCM database. The disease targets of febrile seizures were searched through GeneCards and OMIM databases. Uniport was used to convert protein and gene symbols, and Darw venn diagram platform was used to obtain venn diagram and intersection genes between drug components and diseases. A PPI network was built through the STRING database. Cytoscape3.9.1 software was used to construct the “traditional Chinese medicine-active ingredients-target” network and “key target-signaling pathway” network. The online analysis platform DAVID v6.8 was used to perform KEGG signaling pathway enrichment analysis and GO enrichment analysis of the key targets. The software R x64 4.0.2 and the drawing package ggplot2 were used to plot the KEGG signal path and GO enrichment analysis diagram. Molecular docking was done using AutoDockTools 1.5.7, Pymol 2.0.1 and Openbable 3.1.1 software. Results： There were 8 main effective components of Commelina communis L., which correspond to 140 targets, 2 210 disease targets and 64 common targets of drug diseases. 262 items were obtained by GO enrichment analysis (P<0.05), and 67 signaling pathways were selected by KEGG enrichment analysis (P<0.05). The results of molecular coupling showed that flavonoids, the main component of Commelina communis L., mainly regulating multiple signaling pathways through 3QXY, 1GFW, 2K7w and other targets to play a therapeutic role in the treatment of febrile seizures. Our research provided references and basis for the follow-up research. Conclusion： The flavonoids, β-sitosterol and monoacyl ginsenoside Rb2 in Commelina communis L. can stably bind to the target of febrile seizure disease and have stronger binding ability than phenobarbital, which is used for febrile seizures in clinical, and can treat febrile seizures through multi-component, multitarget, and multi-pathway. It provides new ideas for the research and development of Commelina communis L. and treatment options for hyperthermic seizures. Related Articles | Metrics | Comments（0）

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Efficacy of Feixiankang Granules Combined with Tetrandrine Tablets in Treatment of Pneumoconiosis and Its Effect on Lung Function and Serum IL-8 and IFN-γ Levels QIN Na, WU Xiao-lan, ZHOU Xiao-fan, LONG Zhao, LIAO Zi-jun ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 33-.   DOI: 10.3969/j.issn.2095-1396.2024.01.006 Abstract （62）      PDF（pc） （1074KB）（42）       Knowledge map    Save Objective： To investigate the efficacy of Feixiankang Granules combined with Tetrandrine Tablets in the treatment of pneumoconiosis and its effect on lung function and serum IL-8 and IFN-γ levels. Methods: From June 2021 to May 2023,72 patients with pneumoconiosis admitted to the outpatient or inpatient department of the occupational disease department of Hengyang Hospital of Traditional Chinese Medicine were selected as the research objects. According to the random number table method, the patients were randomly divided into control group(n=36) and observation group (n=36). Both groups were given conventional symptomatic treatment. The control group was treated with tetrandrine tablets on this basis, and the observation group was treated with Feixiankang granules on the basis of the control group. After 3 months of treatment, the clinical efficacy, TCM symptom scores, lung function [maximum ventilation volume (MVV) and forced expiratory volume in one second (FEV1)], serum IL-8, IFN-γ levels and adverse reactions during treatment were compared between the two groups. Results: The total effective rate of the observation group was higher than that of the control group (P<0.05). After treatment, the scores of main symptoms, secondary symptoms and total scores of TCM symptoms in the two groups decreased(P<0.05), and those in the observation group were lower(P<0.05). After treatment, MVV and FEV1 in both groups increased (P<0.05), and those in the observation group were higher(P<0.05). IL-8 decreased in both groups(P<0.05), and the observation group was lower(P<0.05). IFN-γ in both groups increased(P<0.05), and the observation group was higher(P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). Conclusion: Feixiankang granule combined with tetrandrine tablets can significantly improve the curative effect, improve lung function, reduce serum IL-8 level and increase IFN-γ level in patients with pneumoconiosis. Related Articles | Metrics | Comments（0）

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Research Progress on the Correlation Between Heart Failure and Insulin Resistance ZHANG Ze-peng, ZHANG Ai-ai, YANG Bo, LU Meng, LI Fang-jiang ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 59-.   DOI: 10.3969/j.issn.2095-1396.2024.02.010 Abstract （59）      PDF（pc） （1023KB）（17）       Knowledge map    Save In recent years, the treatment of cardiovascular diseases such as hypertension and coronary heart disease has made remarkable progress. However, the incidence of heart failure (HF) remains high and has become an important challenge in the cardiovascular field. At present, the urgent problem is how to effectively delay the occurrence and development of HF and reduce its fatality rate. Recent studies have shown that insulin resistance (IR) is closely related to HF. This article summarized the current research status of the correlation between heart failure and insulin resistance, and provided new ideas and strategies for the treatment of heart failure. The review is now reported as follows. Related Articles | Metrics | Comments（0）

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Study on the Analgesic Effect of Resveratrol in the Context of Pharmacognosy CHEN Yan-ming, CHEN Jin-song ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 6-.   DOI: 10.3969/j.issn.2095-1396.2023.06.002 Abstract （57）      PDF（pc） （1335KB）（10）       Knowledge map    Save Objective: The food and medicine industry is an integral part of the national strategy to develop a “healthy China”. Resveratrol is a naturally occurring compound that has been shown to be biologically active in various diseases such as inflammation, heart disease and cancer. The aim of this study was to investigate the analgesic effects of resveratrol in acute and chronic pain in mice. Methods: In this study, the analgesic effects of resveratrol were evaluated by constructing mouse models of acute pain (acetic acid-induced torsion, formalin-induced persistent pain) and chronic pain (paclitaxel-induced neuropathic pain). Results: The behavioural results showed that resveratrol was effective in reducing acute and chronic pain behaviour in mice and exhibited beneficial effects. Conclusion: Therefore, resveratrol may be a potential drug for the treatment of acute and chronic pain in mice. This study provides a certain theoretical basis for subsequent related studies and offers new ideas and methods for safe and effective clinical analgesia. Related Articles | Metrics | Comments（0）

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Cyclin D1 Expression During Astragaloside A Induced Neuron- Like Cell Differentiation of BMSCs WANG Xiao-yin, WANG Hui-fang, LUO Qiang, ZHANG Hui, ZHANG Xiao-li ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 11-.   DOI: 10.3969/j.issn.2095-1396.2024.01.002 Abstract （56）      PDF（pc） （2593KB）（44）       Knowledge map    Save Objective：To observe the differentiation of bone marrow mesenchymal stem cells (BMSCs) induced by astragaloside A and expression of Cyclin D1. Methods: BMSCs were induced by astragaloside A with 100 mg·L-1. At l, 12, 24 h after induetion, the morphological changes of BMSCs were observed with optical microscope and the expressions of Nestin, NSE, Cyclin D1 were identified by immunocytochemical techniques. Cyclin D1 mRNA was detected by RT-PCR．Results： Bone marrow cells were primarily cultured and subcultured. The third passage of BMSCs was purified. After treatment with 100 mg·L-1 astragaloside A, some cells presented processes, were similar to neuron-like cells in morphology, and expressed Nestin and neuron-specific enolase. Nestin expression occurred earlier (P<0.05) and at a higher level (P<0.05) than expression of neuron-specific enolase. At 1 hour after treatment with astragaloside A, cyclin D1 expression was substantially down-regulated in BMSCs at both gene and protein levels. Simultaneously, BMSCs began to express nestin. No significant differences in cyclin D1 expression in BMSCs were detected between 12 and 24 hours post-treatment and the pretreatment level (P>0.05). Conclusion: Our results indicated that astragaloside A regulated cell cycle, prolonged G1/S phase transition, triggered BMSC differentiation, and finally induced BMSC differentiation into neuronal stem cells. The trophism of astragaloside A accelerated further growth of cell processes and the differentiation of cells into neurons. Related Articles | Metrics | Comments（0）

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Effects of Sacubitril Valsartan on Ventricular Remodeling, Myocardial Fibrosis and Serum Angiogenesis-Related Factors in Patients with Chronic Ischemic Heart Failure WANG Xi, XUE Xia ACTA NEUROPHARMACOLOGICA    2024, 14 (1): 43-.   DOI: 10.3969/j.issn.2095-1396.2024.01.008 Abstract （56）      PDF（pc） （1117KB）（34）       Knowledge map    Save Objective： To investigate the effects of sacubitril valsartan on ventricular remodeling, myocardial fibrosis and serum angiogenesis factors in patients with chronic ischemic heart failure (CIHF). Methods: A total of 200 patients with CIHF were randomly divided into observation group (100 cases) and control group (100 cases), which were treated with sacubitril valsartan and benazepril hydrochloride, respectively. The course of treatment in both groups was 6 months. Observe the curative effect of two groups. The cardiac function indexes [left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDD)], 6-minute walking distance (6MWT), Minnesota Living with Heart Failure Questionnaire (MLHFQ) score, myocardial fibrosis indexes [transforming growth factor-β1 (TGF-β1), soluble growth stimulating gene 2 protein (sST2)] and angiogenesis factors [vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), angiotensin-1 (Ang 1)] were statistically analyzed before and after treatment in the two groups. Results: Comparing with the control group (71.13%), the observation group's total effective rate (86.46%) was higher (P<0.05). After treatment, the levels of NT-proBNP, LVEDD and MLHFQ score in the observation group were significantly lower comparing with the control group (P<0.05), while LVEF and 6MWT were significantly higher (P<0.05). After treatment, the serum levels of TGF-β1, sST2 and TIMP-1 in the observation group were significantly lower comparing with the control group (P<0.05), and the levels of VEGF, IGF-1 and Ang 1 were significantly higher (P<0.05). Conclusion: For CIHF, sacubitril valsartan can effectively regulate myocardial fibrosis indexes and angiogenesis factors, reverse ventricular remodeling, improve cardiac function and quality of life of patients, and has a good effect. Related Articles | Metrics | Comments（0）

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Research Progress of Core Markers and Other Markers of Alzheimer's Disease YIN Hong-yan, YOU Si-han, GUO Chun-yan ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 45-.   DOI: 10.3969/j.issn.2095-1396.2023.06.009 Abstract （55）      PDF（pc） （976KB）（15）       Knowledge map    Save Alzheimer's disease （AD） is a neurodegenerative disease that cannot be cured until now. The main existing diagnostic methods are the detection of biomarkers in cerebrospinal fluid and positron emission tomography （PET）， but their application in clinical practice is hampered due to the invasiveness and high cost of their testing. Biomarkers in blood samples can make early detection and diagnosis of ad universal because of their easy availability and low price. This article summarizes several common biomarkers of AD and their changes in AD patients， and prospects the development of ad biomarkers in the future. Related Articles | Metrics | Comments（0）

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Research Status on the Neuroprotective Effect of Probucol ZHU Jing-yi , FAN Jian-chun, YU Ai-mei ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 50-.   DOI: 10.3969/j.issn.2095-1396.2023.06.010 Abstract （55）      PDF（pc） （926KB）（12）       Knowledge map    Save Probucol has two easily oxidized phenolic hydroxyl groups， which can combine with oxygen free radicals to form a stable phenolic oxygen group. It has powerful lipid-lowering， anti-inflammatory and antioxidant functions and plays an important role in the treatment process. This article reviews the protective effects and detailed protective mechanisms of probucol in the central nervous system and peripheral nervous system based on domestic and foreign literature in recent years. Related Articles | Metrics | Comments（0）

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Research Progress of TRIM32 and Related Nervous System Diseases SUN Xiao-jing, MENG Xian-yong, YU Hui-jiao, DONG Xiao-hua ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 47-.   DOI: 10.3969/j.issn.2095-1396.2024.02.008 Abstract （53）      PDF（pc） （1032KB）（13）       Knowledge map    Save Tripartite motif proteins (TRIM) are a class of proteins involved in a wide range of biological processes, including cell growth and differentiation, development, apoptosis, immune response, and tumorigenesis. Among them, TRIM32 is an E3 ubiquitin ligase that belongs to the TRIM protein family. Although there are few studies on TRIM32, its role in maintaining the physiological function of the nervous system is extremely important. Therefore, this review aims to review the role of TRIM32 in neurological diseases. Related Articles | Metrics | Comments（0）