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    Research Progress on the Etiology of Febrile Seizures and the Treatment
    HAO Xue-nan, YANG Lei, ZHAO Wen-ya, ZHANG Wei
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 42-.   DOI: 10.3969/j.issn.2095-1396.2024.04.008
    Abstract161)      PDF(pc) (1059KB)(52)       Save
    Febrile seizures (FS) are convulsions characterized by a body temperature of 38.5℃ or higher, without central nervous system infection or other epileptic causes, affecting 2% to 14% of the global population and being a common neurological disorder in childhood. The occurrence of FS is age dependent, most prevalent in children aged 6 months to 5 years. The pathogenesis of FS remains unclear, although it is known that various factors can induce FS, including abnormal body temperature, age and brain maturity, genetic factors, and infections. Moreover, other factors can predispose to the development of FS. Current treatment of FS primarily involves antiepileptic drugs. This article reviews the pathophysiological mechanisms of FS, with a focus on the research progress of FS etiology and treatment.
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    Effects of Tau Protein on Mitochondrial Function in Alzheimer's Disease
    YAO Si-fan, ZHANG Xin, SHEN Li-xia
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 54-.   DOI: 10.3969/j.issn.2095-1396.2024.04.010
    Abstract149)      PDF(pc) (1084KB)(571)       Save
    Tau protein is an important protein that physiologically promotes microtubule assembly and stabilization and is involved in neuronal development, axonal transport and neuronal polarity. In Alzheimer's disease (AD), Tau proteins undergo pathological modifications in which soluble Tau assembles into insoluble filaments, leading to synaptic failure and neurodegenerative lesions. Mitochondria are the most important organelles in neurons and are the main source of energy, providing adenosine triphosphate (ATP) through oxidative phosphorylation to maintain normal neuronal homeostasis and function. A growing body of evidence suggests that mitochondrial dysfunction plays a key role in the pathogenesis of AD. Negative effects on mitochondrial bioenergetics, transport and morphology in neurons lead to synaptic damage and cognitive decline in AD. Tau proteins have been shown to interact with mitochondrial proteins, impairing mitochondrial dynamics, mitochondrial morphology and autophagy, leading to neurotoxicity. This article provides a review of the effects of Tau proteins on mitochondria in AD and the mechanisms of action.
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    To Investigate the Mechanism of Taurine for Age-Related Macular Degeneration Based on Network Pharmacology and Molecular Docking
    CHEN Xiao-yang, WANG Zi-yi, XIE Ya-hao, JIN Qi, LIU Si-ning, AN Jia-yi, LIU Ya-meng, QIANG Meng-ze, LI Jia-geng, CHEN Zhuo-zhuo, ZHANG Yuan-yuan, YU Jia-chen, YANG zhi-qiang
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 1-.   DOI: 10.3969/j.issn.2095-1396.2024.06.001
    Abstract138)      PDF(pc) (2812KB)(185)       Save
    Objective: To investigate the effect and mechanism of Taurine in the treatment of age-related macular degeneration (AMD) by using network pharmacology and molecular docking technology and in vitro experiments. Methods: The intersection targets of Taurine and AMD were obtained and screened from different databases. Through the Protein Interaction Network (PPI). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG Enrichment Analysis) signal pathway analysis, according to Cytoscape to establish a “drug-target-pathway” network to deeply explore the mechanism of Taurine action on AMD. Preliminary verification by molecular docking technology. The model of ARPE-19 cell senescence was established by H2O2 in vitro. Cell proliferation assay kit (CCK-8) was used to detect the optimal concentration and action time of H2O2 and Taurine. The senescence degree was detected by β-galactosidase kit staining. The levels of steroid receptor co-activating protein (SRC), tumor protein 53 (P53) ,and myeloperoxidase (MPO) were detected by Western blotting (WB) assay. The expression levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by ELISA kit. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to analyze the expression levels of target genes SRC, P53 and MPO mRNA. Results: Network pharmacological analysis showed that the intersection of Taurine and AMD targets included inflammation-related factors such as SRC, IL-6, TNF-α, and cell senescence related targets such as P53 and MPO. The results of GO biological function and KEGG pathway enrichment analysis show that it contains signaling pathways related to aging inflammation. The results of β-galactosidase staining and CCK-8 experiment showed that the optimal concentration of H2O2 on APRE-19 cells was 100μmol·L-1 for 24 h, and Taurine 10 mmol·L-1, 20 mmol·L-1 and 40 mmol·L-1 were suitable concentrations. WB results showed that SRC was significantly increased and P53 and MPO were significantly decreased in Taurine group compared with model group. PCR results showed that compared with the model group, mRNA level of SRC was significantly increased, and P53 and MPO mRNA levels were significantly decreased in Taurine group. ELISA results showed that IL-6 and TNF-α were significantly decreased in Taurine group compared with model group. Conclusion: Network pharmacology, molecular docking and cell experiments were used to confirm that Taurine has an anti-aging effect on ARPE-19 cells by inhibiting inflammation through the SRCP53- MPO axis. It provides a scientific basis for further elucidation of the mechanism of action of taurine in the treatment of AMD.
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    Research Progress of GABAA Receptor and Central Nervous System Diseases
    ZHANG Ran-ran, ZHANG Chun-yue, WU Zhi-gang
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 50-.   DOI: 10.3969/j.issn.2095-1396.2024.06.008
    Abstract125)      PDF(pc) (982KB)(278)       Save
    GABAA receptors are pentamers composed of multiple subunits, which not only determine the cellular location of GABAA receptors but also affect their biological functions. When the subunit is mutated, the receptor cannot effectively respond to GABA released by presynaptic neurons and glia, resulting in central nervous system diseases. This article will review the relationship between GABAA receptor and common diseases of central nervous system, and strive to provide theoretical basis for the treatment of these diseases.
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    Study on the Effects of Vitamin D on DNA Methylation in Alzheimer's Disease Cell Models
    ZHANG Yuan-qing, LIN Yu, HUANG Li
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 39-.   DOI: 10.3969/j.issn.2095-1396.2024.06.006
    Abstract119)      PDF(pc) (1014KB)(83)       Save
    Objective: This study aims to investigate the effects of Vitamin D (Vit D) on DNA methylation in Alzheimer's Disease (AD) cell models, and the relationship between these effects and AD pathological progression, to provide new insights and potential targets for AD prevention and treatment. Methods: An AD cell model was constructed by treating human neuroblastoma SH-SY5Y cells with Aβ1-42 (10 μmol·L-1) for 24 hours. Western blot was used to detect p-tau protein expression to validate the model. Cells were divided into normal control group, AD model group, and high, medium, and low concentration Vit D treatment groups. MTT assay was used to evaluate the effect of Vit D on cell proliferation. Real-time PCR and Western blot were used to detect mRNA and protein expression levels of DNA methyltransferases, respectively. Results: The AD model group showed significantly increased p-tau protein expression. Vit D treatment groups demonstrated significantly improved cell proliferation rates compared to the AD model group, in a dose-dependent manner. Compared with the AD model group, the mRNA and protein expression levels of DNMT1 and DNMT3A were significantly upregulated in the Vit D treatment groups. Conclusion: This study reveals that Vit D improves pathological changes in AD cell models by upregulating DNMT1 and DNMT3A expression, thereby increasing DNA methylation levels. This finding provides a new perspective for understanding the protective mechanism of Vit D in AD, while also offering potential epigenetic targets for the development of AD prevention and treatment strategies.
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    Expression of CAV1 and CAV2 in Head and Neck Squamous Cell Carcinoma Tissues and Their Effects on Survival, Immunity
    CAO Xin-ran, FAN Jian-chun, WANG Xia, LIU Xun-tao, ZHANG Bin
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 9-.   DOI: 10.3969/j.issn.2095-1396.2024.04.002
    Abstract113)      PDF(pc) (3364KB)(11)       Save
    Objective: The key biomarkers CAV1 and CAV2 of HNSCC were screened based on bioinformatics methods, and their expression in HNSCC and clinical prognostic value were explored. Methods: In this study, we first compared the differential expression of caveolins and cavins in HNSCC based on the GEPIA database, and then simultaneously analyzed the relationship between the differential genes and clinical phenotypes in the caveolins and cavins families in the Ualcan platform; compared the relationship between the differential genes associated with the clinical phenotypes and the survival of HNSCC; and in addition performed CAV1 and CAV2 enrichment analysis and immune infiltration analysis were performed. Results: GEPIA database analysis showed significant correlation between CAV1, CAV2, CAVIN2,CAVIN3, etc. Among them, CAV1 and CAV2 were significantly correlated with the clinical phenotype and could significantly affect the survival and prognosis of HNSCC patients. GSEA enrichment analysis showed that CAV1 mainly upregulated IL-17 signaling pathway, PI3K-Akt signaling pathway and NOD-like receptor signaling pathway; CAV2 mainly upregulated NODlike receptor signaling pathway and PI3K-Akt signaling pathway. signaling pathway, PI3K-Akt signaling pathway, and NOD-like receptor signaling pathway; CAV2 mainly up-regulated NODlike receptor signaling pathway and PI3K-Akt signaling pathway; and immune infiltration analysis showed that CAV1 and CAV2 significantly altered the survival and prognosis of HNSCC patients. CAV2 significantly altered the immune microenvironment of HNSCC tumors and correlated with the infiltration of various immune cells. Conclusion: CAV1 and CAV2, which are highly expressed in HNSCC, are independent prognostic factors associated with immune invasion and can be used as candidate prognostic markers for determining immune invasion-related prognosis in patients with HNSCC.
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    Recent Progress in the Metabolomics of Alzheimer's Disease
    ZHANG Xin, YAO Si-fan, MA Meng-fan, SHEN Li-xia
    ACTA NEUROPHARMACOLOGICA    2024, 14 (5): 47-.   DOI: 10.3969/j.issn.2095-1396.2024.05.007
    Abstract108)      PDF(pc) (983KB)(26)       Save
    Alzheimer's disease is a complex neurodegenerative disorder whose exact etiology is still not fully understood. In recent years, the application of metabolomics has provided new perspectives to unravel the complex biological mechanisms of Alzheimer's disease. By quantitatively analyzing a comprehensive range of metabolites in biological samples, researchers are able to identify metabolic changes associated with Alzheimer's disease, providing data to support the exploration of potential diagnostic markers and therapeutic targets. This paper reviews research advances in metabolomics in revealing metabolic changes, identifying biomarkers, and detection tools for Alzheimer's disease. Metabolomics studies have confirmed the presence of reduced brain energy metabolism, abnormal membrane phospholipid metabolism, and imbalances in pathways related to lipid, amino acid, and choline metabolism in patients with AD. These findings emphasize the importance of metabolomics in AD and provide valuable information for future research directions and clinical diagnosis.
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    Exploring the Mechanism of Action of Astragalus-Curcuma Zedoary in Ameliorating Central Neuroinflammation Based on Network Pharmacology Combined with Molecular Docking
    YANG Xian-cui, ZHAO Bei
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 13-.   DOI: 10.3969/j.issn.2095-1396.2024.06.002
    Abstract100)      PDF(pc) (3300KB)(954)       Save
    Objective: To investigate the potential targets and signaling pathways of Astragalus-Curcuma zedoary in ameliorating central nervous system inflammation based on network pharmacology and molecular docking. Methods: The main active ingredients and targets of the “Astragalus-Curcuma zedoary in” pair were searched in TCMSP database and BATMANTCM database, and the targets of CNS inflammation were screened in GeneCards database, and the network diagram of “drug-ingredient-target” was established by Cytoscape 3.7.1. Cytoscape 3.7.1 was used to establish the network diagram of “drug-component-target”, and venny 2.1.0 was used to do the Wayne diagram intersection plot to obtain the intersection genes of Astragaluscurcuma zedoary in CNS inflammation. We used String database to obtain the protein interactions map, and analyzed the GO function enrichment and KEGG pathway enrichment of the targets by DAIVID, an online analysis platform, to screen out the functions of the target proteins and their related mechanism pathways. Results: Astragalus-curcuma zedoary contains 129 important active ingredients including quercetin, kaempferol, curcumol, and soybean sapogenins, which act on 543 potential targets for drug therapy of diseases including AKT1, STAT3, IL-6, IL-1β, TNF, BCL- 2, IFNG, and JUN. GO and KEGG enrichment analyses revealed that these targets were widely enriched in HIF-1, TNF, and IL-1β, and HIF-1, TNF and IL-1β. The GO and KEGG enrichment analyses revealed that these targets were widely enriched in a series of signaling pathways, including HIF-1, TNF, and IL-17, and the core active ingredients were also tightly bound to the core targets. Conclusion: Astragalus-curcuma zedoary may regulate the HIF-1, TNF, and IL-17 pathways through the core targets of AKT1, STAT3, IL-6, IL-1β, TNF, BCL-2, IFNG, and JUN, which may play an ameliorative role in CNS inflammation, and thus provide scientific basis and reference for the treatment of CNS inflammation by traditional Chinese medicine.
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    Analysis of Clinical Efficacy of Memantine Hydrochloride in the Treatment of Parkinson's Dementia Patients
    LIU Chang-jia, YAN Kun
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 36-.   DOI: 10.3969/j.issn.2095-1396.2024.06.005
    Abstract95)      PDF(pc) (994KB)(52)       Save
    Objective: To explore the application effect of memantine hydrochloride in the adjuvant treatment of Parkinson 's dementia. Methods: 60 patients with Parkinson 's dementia were randomly divided into control group (donepezil hydrochloride group) and observation group (memantine hydrochloride group), 30 cases in each group. Results: The total effective rate of the observation group was significantly higher than that of the control group (P<0.05), and the MMSE scale score was higher than that of the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: The clinical effect of memantine hydrochloride in the adjuvant treatment of Parkinson's dementia is significant, which is helpful for patients to restore their daily living ability and cognitive function as soon as possible. It is safe and worthy of clinical application.
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    Analysis and Evaluation of Opioid in the Inpatients During 2021- 2023 in the First Affiliated Hospital of Hebei North University
    WANG Ze-yu, WANG Yu, QIAO Ting-ting, MA Huan, HAO Xiu-qing, ZHENG Mao-Dong, YAN Juan
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 21-.   DOI: 10.3969/j.issn.2095-1396.2024.06.003
    Abstract88)      PDF(pc) (1280KB)(117)       Save
    Objective: To analyze the application of opioids during 2021-2023 in the inpatients in the first affiliated hospital of Hebei north university, and to evaluate rationality of opioids use, so as to provide reference for promoting the rational use of opioids in the first affiliated hospital of Hebei north university. Methods:To refer to the application of opioids between January 2021 and December 2023 in the inpatients of the first affiliated hospital of Hebei north university. Graph Pad Prism 8 software was used to retrospective analyze and evaluate that the consumption volume, consumption sum, DDDs, DDC, B/A, distribution of departmental and disease use of opioids. Graph Pad Prism 8 software was used for linear regression analysis, and the trend of drug use with each year was estimated according to the regression coefficient of the mode. SPSS 18.0 software was used to analyze the chisquare test for quantitative indicators. Results:Thirteen opioids were included in this study. The top 5 opiods in the list of consumption volume were Oxycodone hydrochloride sustained-release tablets (40 mg), Morphine sulfate sustained-release tablets, Bucinnazine hydrochloride injection, Compound platycodon tablets and Oxycodone hydrochloride sustained-release tablets (10 mg) of the first affiliated hospital of Hebei north university during 2021-2023. The use of oral dosage forms, injectable dosage forms, and patches of opioids increased yearly and were considered as statistically significant(P<0.05). Oxycodone hydrochloride sustained-release tablets (40 mg) consumption ratio trends upward. The top 3 opiods in the list of DDDs were Oxycodone hydrochloride sustained-release tablets (40 mg), Morphine sulfate sustained-release tablets and Bucinnazine hydrochloride injection of the first affiliated hospital of Hebei north university during 2021-2023. Opioids DDDs were in an increasing trend (β>0) and were considered as statistically significant (P<0.05), except for codeine phosphate tablets, morphine hydrochloride tablets, Bucinnazine hydrochloride tablets. The top 5 opiods in the list of consumption sum were Oxycodone hydrochloride sustained-release tablets (40 mg), Morphine sulfate sustainedrelease tablets, Oxycodone hydrochloride sustained-release tablets (10 mg), Fentanyl Citrate Injection and Remifentanil hydrochloride for Injection of our hospital during 2021-2023. Opioids consumption sum were in an increasing trend (β>0) and were considered as statistically significant (P<0.05), except for codeine phosphate tablets, morphine hydrochloride tablets, Bucinnazine hydrochloride tablets. All of opioids DDC trends upward during 2021-2023. Remifentanil Hydrochloride for Injection, Oxycodone hydrochloride sustained-release tablets (10 mg) and Oxycodone hydrochloride sustained-release tablets (40 mg) DDC were consistently above 60. Bucinnazine hydrochloride Injection, Codeine phosphate tablets, Bucinnazine hydrochloride tablets and compound platycodon tablets B/A were above 1.00, Fentanyl Citrate Injection B/A were above 2.00. Other opioids B/A remained largely unchanged at 0.00~1.50. Chinese medicine, medical oncology, and respiratory and critical care medicine departments frequently uesd opiods during 2021-2023. Tumors used opioids Most, and lung cancer had highest use rate of opioids. Conclusion:Consumption volume, DDDs, consumption sum and DDC of opioids of the first affiliated hospital of Hebei north university were increasing yearly. Patients with tumors was a major group of opioid users. We should deeply study pharmacogenomics and realize individualized medication of opioids. To select the appropriate drug for the patient in order to use the drug accessibility, safety, and effectiveness.
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    Research Progress in the Treatment of Thin Endometrium with Traditional Chinese Medicine
    LI Na, ZHAO Cui-ying
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 49-.   DOI: 10.3969/j.issn.2095-1396.2024.04.009
    Abstract85)      PDF(pc) (1012KB)(28)       Save
    Thin endometrium is one of the important causes of menorrhagia, recurrent abortion, infertility and other diseases, which is difficult to treat and difficult to detect in the early stage. By reviewing the research literature in recent years, the author found that traditional Chinese medicine has good clinical efficacy and few side effects in the treatment of thin endometrium. It can not only directly promote the expression of related factors, inhibit inflammatory factors, regulate the level of estrogen in the body, promote cell proliferation or apoptosis, increase endometrial receptivity, but also regulate a variety of signal pathways, multi-target, all-round treatment of TE. The current clinical research shows that simple internal administration of traditional Chinese medicine, simple external treatment, internal and external combination of traditional Chinese medicine and the combination of traditional Chinese and western medicine can effectively treat thin endometrium and improve the clinical symptoms of patients.
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    Progress in the Use of Oral Chemotherapeutic Agent Xeloda
    JIA Xiao-kun
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 58-.   DOI: 10.3969/j.issn.2095-1396.2024.06.009
    Abstract84)      PDF(pc) (1094KB)(287)       Save
    Xeloda, also known as capecitabine tablets, is the first oral fluorouracil chemotherapy drug, which is converted into 5-fluorouracil(5-FU) by intestinal absorption, and 5-FU is converted into deoxyuridylate monophosphate (FdUMP) and 5-fluoruridylate triphosphate (FUTP) by cell metabolism. It can well simulate the state of 5-FU intravenous therapy, with high effectiveness. Convenience and high efficiency are often used in adjuvant therapy, combined with paclitaxel and other drugs to treat breast cancer, gastric cancer and other diseases, but there are still many adverse reactions. This article reviews the structure, function, clinical application and clinical effect of oral chemotherapy Xeloda.
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    Research on the Optimization of Mistletoe Extraction Process Based on Box-Behnken Design-Response Surface Method
    ZHANG Ao-kai, LI Wen-jie, DONG Yang-yang, CHU Han-bing, DONG Xue-hai, ZHAO Li-yan, ZHANG Wanming , ZHANG Yuan-yuan
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 1-.   DOI: 10.3969/j.issn.2095-1396.2024.04.001
    Abstract82)      PDF(pc) (2015KB)(203)       Save
    Objective: To optimize the extraction process of Mistletoe by using the Box-Behnken design-response surface method, and the comprehensive content of the two active ingredients as the evaluation index. Methods: The ethanol volume fraction, extraction time and solid-liquid ratio of Mistletoe extraction process were optimized by using the Box- Behnken design-response surface method on the basis of single factor. Ultra performance liquid chromatography (UPLC) was used to determine the content of two active components in Mistletoe from 14 origins. Results: The optimal extraction process of Mistletoe was 71% ethanolaqueous solution, liquid-solid ratio 25:1, and extraction time 67 min. The optimal extraction process showed that the average comprehensive content was 571.7 μg·g-1 when the weight ratio of Syringin to homoeriodictyol-7-O-β-D-glucoside was 7:3. The contents of Syringin and homoeriodictyol-7-O-β-D-glucoside ranged from 128.7~662.8 μg·g-1 and 77.24~801.8 μg·g-1. The contents of Syringin and homoeriodictyol-7-O-β-D-glucoside in Mistletoe from different origins were different, among which the content of homoeriodictyol-7-O-β-D-glucoside in Mistletoe from Sichuan was relatively high, which was 0.84 mg·g-1, and the content of Syringin from Mistletoe from Anhui was relatively high at 0.47 mg·g-1. Conclusion: The method established in this study has high sensitivity, good separation effect, stable and reliable results, which can provide a scientific basis for the extraction of active ingredients, quality evaluation and resource development and utilization of Mistletoe.
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    Rationality and Standardization Analysis of Benzodiazepines in Chronic Insomnia Patients
    HUANG Jun-hua
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 38-.   DOI: 10.3969/j.issn.2095-1396.2024.04.007
    Abstract79)      PDF(pc) (1063KB)(23)       Save
    Objective: To analyze the rationality and standardization of benzodiazepine drugs in chronic insomnia patients. Methods: 148 patients with chronic insomnia from November 2022 to November 2023 were selected, the electronic prescription data were collected, the use of benzodiazepines was observed, and the rationality and standardization of benzodiazepine use were evaluated. Results: 55 of the 148 patients were on benzodiazepines, alprazolam tablets were the most prescribed, for 48 sheets (32.43%), 5 clonazepam tablets (3.38%), 2 diazepam tablets (1.35%). The majority of chronic insomnia patients were prescribed non-benzodiazepines (93, 62.84%). In the non-standard situation of benzodiazepine drugs, the prescription writing is not standardized. Repeated drug use, too long drug use time, inappropriate scope of drug use, incompatibility and other conditions appear less. Drug utilization index (DUI) of alprazolam tablets, clonazepam tablets and diazepam tablets were <1, Drug use is justified. Conclusion: The overall process of benzodiazepine treatment for chronic insomnia patients in our hospital is reasonable and normative, and some deficiencies need to be further improved.
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    Network Pharmacology Study of Sini SAN in the Treatment of Post-Stroke Depression
    ZHOU Xin-mei, ZHU Li-ping
    ACTA NEUROPHARMACOLOGICA    2024, 14 (5): 19-.   DOI: 10.3969/j.issn.2095-1396.2024.05.003
    Abstract77)      PDF(pc) (4308KB)(808)       Save
    Objective: To explore the molecular mechanism of Sini SAN in the treatment of post-stroke depression based on network pharmacology and molecular docking techniques. Methods: Active ingredients and therapeutic targets in Sini SAN were searched through TCMSP database. Gene targets of post-stroke depression were collected using GeneCards, OMIM and PharmGkb databases. The intersection targets were selected as potential targets and imported into String database to establish protein interaction (PPI) network. Network topology analysis by Cytoscape software. PPI targets were introduced into R for gene ontology (GO) function and Kyoto Encyclopedia of Genomes (KEGG) pathway enrichment analysis. The “pathway-target” network was constructed and the key targets were screened, and the AMDock platform was used to interconnect the target with the active ingredient to screen the potential pharmacodynamic ingredient.Results: 104 kinds of drug chemical components, 235 targets and 1 001 disease target genes of Sini SAN were obtained from the database, including 7 kinds of drug core components and 77 overlapping genes with diseases. Through software analysis, MMP9, AKT1, IL-10, ESR1, IL-6, HIF1A, IL-1B, CASP3 and TNF were the core genes. GO enrichment analysis showed that it was mainly through the molecular functions of protease binding, nuclear receptor activity, cytokine activity, estrogen response element binding, etc., in the membrane raft, membrane microregion, plasma membrane raft, Bcl-2 family protein complex, plasma membrane lateral, neuron cell body, endoplasmic reticulum cavity and other action sites. The reaction to the decrease of oxygen content, the regulation of apoptosis signaling pathway, the reaction to lipopolysaccharide and other processes play a role. KEGG analysis showed AGE-RAGE signaling pathway, p53 signaling pathway, TNF signaling pathway, estrogen signaling pathway, HIF-1 signaling pathway and other major signaling pathways. Conclusion: Sini SAN may act on MMP9, IL-6, HIF1A, IL-1B, CASP3 and other potential targets through various active ingredients such as quercetin, luteolin, kaempferol, chenetin, naringin, isorhamnetin, and ononin. It mediates AGE-RAGE signaling pathway, estrogen signaling pathway, HIF-1 signaling pathway and other signaling pathways to play an effective role.
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    Effects of Carbazochrome Sodium Sulfonate for Injection Combined with Tranexamic Acid on Perioperative Blood Loss, Coagulation Indexes and Inflammatory Factors in Patients with Femoral Neck Fracture
    ZHONG Jian-yuan, GAO Xiao-le
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 45-.   DOI: 10.3969/j.issn.2095-1396.2024.06.007
    Abstract76)      PDF(pc) (1025KB)(41)       Save
    Objective: To investigate the effect of carbazochrome sodium sulfonate for injection combined with tranexamic acid on perioperative blood loss in patients with femoral neck fracture. Methods:A total of 120 patients with femoral neck fracture undergoing surgical treatment were randomly divided into control group and observation group, with 60 cases in each group. Five minutes before skin incision, both groups were given intravenous infusion of tranexamic acid 1 g. Immediately after the operation, the observation group was given intravenous infusion of carbazochrome sodium sulfonate for injection, while the control group was given intravenous infusion of normal saline. The perioperative blood loss of the two groups was recorded. The levels of fibrinogen degradation product (FDP) and D-dimer in peripheral blood of the patients were detected before and 1 day after operation, and the levels of serum interleukin-6 (IL-6) and C-reactive protein (CRP) were detected. The postoperative hospitalization time and complications of the two groups were recorded. Results: Compared with the control group, the total blood loss and hidden blood loss in the observation group were decreased (P<0.05), and the postoperative drainage volume, maximum Hb decrease value and allogeneic blood transfusion were significantly decreased (P<0.05). One day after operation, the levels of FDP, D-dimer, IL-6 and CRP in the two groups increased (P<0.05), but compared with the control group, the levels of FDP, D-dimer, IL-6 and CRP in the observation group were lower(P<0.05). Compared with the control group, the postoperative hospital stay in the observation group was shorter(P<0.05). There was no significant difference in the incidence of infection and lower extremity intermuscular venous thrombosis between the two groups (P>0.05). Conclusion:The use of carbazochrome sodium sulfonate for injection combined with tranexamic acid can reduce perioperative blood loss, inhibit fibrinolysis, reduce inflammation, shorten postoperative hospital stay, and do not increase complications in patients with femoral neck fracture.
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    MRI Perfusion Imaging Evaluation of Butylphthalide Injection Combined with Xuesaitong Injection in The Treatment of Acute Cerebral Infarction
    XIAN Yao-lin, HUANG Rong-rong
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 23-.   DOI: 10.3969/j.issn.2095-1396.2024.04.004
    Abstract74)      PDF(pc) (1657KB)(11)       Save
    Objective: To observe the clinical efficacy of butylphthalide injection combined with Xuesaitong Injection in the treatment of acute cerebral infarction, and to evaluate the efficacy from the perspective of MRI perfusion imaging. Methods: 80 patients with acute cerebral infarction who met the inclusion criteria were randomly divided into control group and treatment group according to the random number table method, 40 cases in each group. After admission, the patients in both groups were treated according to the clinical pathway, and were given drugs such as antihypertensive, hypoglycemic, lipidlowering, neurotrophic, and antiplatelet aggregation. The control group was treated with butylphthalide sodium chloride injection 100 mL intravenous drip on this basis, while the observation group was treated with Xuesaitong injection 200 mg and 5% glucose injection 250 mL intravenous drip on the basis of the control group. The National Institutes of Health Stroke Scale (NIHSS) score, Barthel Index (BI) of activities of daily living score and MRI perfusion imaging related indicators were compared between the two groups on the day of admission and 10 days after treatment. Results: The total effective rate of the treatment group was significantly higher than that of the control group (97.50% vs 77.50%, P<0.05); After treatment, the NIHSS score of the two groups decreased and the Bi score increased compared with that before treatment, and the difference was statistically significant (P<0.05), and the treatment group was better than the control group (P<0.05); After treatment, the attenuation index of the region of interest in both groups was significantly reduced, and the average diffusion coefficient was significantly increased (P<0.05), and the data of the treatment group were significantly better than those of the control group (P<0.05); After grouping treatment, the cerebral blood volume and cerebral blood flow of the treatment group were significantly better than those of the control group (P<0.05); According to Pearson correlation analysis, the average diffusion coefficient (r=-0.503, r=0.482), attenuation index (r=-0.487, r=0.414), cerebral blood volume (r=-0.527, r=0.402) and cerebral blood flow (r=-0.625, r=0.516) in the treatment group after treatment were significantly correlated with NIHSS score and Bi score (P<0.05). Conclusion: Based on the results of multimodal MRI imaging, it can confirm the good effect of butylphthalide injection combined with Xuesaitong Injection in the treatment of acute cerebral infarction, show the difference changes of average diffusion coefficient and attenuation index in the lesion area before and after treatment, intuitively reflect the changes of volume ratio anisotropy and partial anisotropy, as well as the improvement of cerebral blood flow and cerebral blood volume, and the changes of the above indicators have a good correlation with the degree of neurological deficit and the changes of daily living ability in patients with acute cerebral infarction, suggesting that the results of multimodal MRI imaging can provide a reliable reference for the evaluation of the curative effect of acute cerebral infarction.
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    Determination of Hypoxanthine and Inosine in Five Origins of Geosaurus by HPLC Method
    WANG Hui, WANG Mai, MEI Yan-fei, SHI Yu-jie
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 18-.   DOI: 10.3969/j.issn.2095-1396.2024.04.003
    Abstract73)      PDF(pc) (1161KB)(21)       Save
    Objective: To establish a method for the determination of hypoxanthine and inosine content in Geosaurus, and to determine the constituents of Geosaurus of five origins, so as to explore a new and efficient analytical research method for the study of extraction process and quality analysis of Geosaurus 's constituents. Methods: A high performance liquid chromatographic (HPLC) method was established for the determination of hypoxanthine and inosine. The separation was carried out on an Agilent extend C18 column, 4.6 mm×250 mm, particle size: 5 μm, with the mobile phase of methanol -0.2% phosphoric acid, injection volume of 20 μL, wavelength of 254 nm, column temperature of 30 ℃ , and gradient elution, to establish the standard curves of hypoxanthine and inosine and carry out the methodology of precision, stability,reproducibility, and sample recovery. The methodology of precision test, stability test, repeatability test and sample recovery test was investigated. Results: The standard curve of hypoxanthine: y = 78.819x + 245.92, R2 = 0.999 9, with good linearity in the range of 10.08~161.28 μg·mL-1; the standard curve of inosine: y = 56.566x + 68.333, R2 = 0.999 5, with good linearity in the range of 20.08~321.28 μg·mL-1; the standard curve of inosine: y = 56.566x + 68.333, R2 = 0.999 5, with good linearity in the range of 20.08~321.28 μg·mL-1. The linear relationship was good in the range of 20.08~321.28 μg·mL-1. The average recovery of hypoxanthine was 95.43% with the RSD value of 1.52% (n=6); the average recovery of inosine was 99.86% with the RSD value of 2.36% (n=6); and the contents of hypoxanthine in the five types of Geosaurus of Guangdong, Henan, Hainan, Shanghai and Guangxi were 0.533 5, 0.346 8, 0.687 4, 0.687 4, 0.566x + 68.333, R2= 0.999 5, with good linear relationship in the range of 20.08~321.28 μg·mL-1. The contents of hypoxanthine in Geosaurus from Guangdong, Henan, Hainan, Shanghai and Guangxi were 0.533 5, 0.346 8, 0.687 4, 0.569 4, 0.794 5 (mg·g-1), and the contents of inosine were 1.562 5, 0.567 4, 1.126 7, 0.843 6, 1.284 9 (mg·g-1). Conclusion: The established HPLC method of high performance liquid chromatography (HPLC) has a good gradient elution effect, good experimental accuracy, good precision, good stability and high reproducibility, which is an efficient analytical determination.
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    A Case of Huntington's Disease with Involuntary Movements of the Limbs as the First Symptom Combined with Dysarthria in the Early Stages of the Disease
    LI Hui, YANG Jin-shui, XUE Qian
    ACTA NEUROPHARMACOLOGICA    2024, 14 (6): 31-.   DOI: 10.3969/j.issn.2095-1396.2024.06.004
    Abstract69)      PDF(pc) (1174KB)(127)       Save
    Objective: To investigate the clinical characteristics of patients with Huntington's disease patients, especially the atypical clinical symptoms, to deepen the understanding of the clinical characteristics of Huntington's disease. Methods: The clinical data of one HD patient admitted to the Department of Neurology of the First Hospital Affiliated to Hebei North College in January 2022 were collected under the principle of patient's informed and voluntary consent, and the relevant literature was reviewed and followed up. Results: Two members of this patient's family had already developed symptoms of involuntary limb movements, and this pre-documented person had early comorbid dysarthria with a CAG copy number of 43, and it was understood from the relevant literature review that speech alterations are an early symptom of HD, which may precede the onset of other motor and cognitive symptoms. Conclusion: The vast majority of HD has a positive family history and is clinically characterised by slowly progressive chorea, psychotic disorders and dementia, with speech alterations in the early stages and dysarthria and dysphagia in the later stages.
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    To Observe the Effect of Yishen-Buganjiangu Decoction Combined with Ecalcitonin in the Treatment of Primary Osteoporosis and Its Influence on Bone Metabolism and Oxidative Stress
    DAI Xiu-xia, LIN Hai-yang, SU Zi-zhen, QIU Ying-jie, SU Li-yun, CHEN Li-feng
    ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 29-.   DOI: 10.3969/j.issn.2095-1396.2024.04.005
    Abstract64)      PDF(pc) (1089KB)(18)       Save
    Objective: To investigate the effect of Yishen-Buganjiangu decoction combined with ecalcitonin on primary osteoporosis and its influence on bone metabolism and oxidative stress. Methods: Eighty patients with osteoporosis were divided into a control group and an observation group. The control group received intramuscular injection of ecalcitonin, 10 IU·time-1, twice a week. The observation group was treated with Yishen Bugan Jiangu decoction on the basis of the control group. Both groups were treated for 6 months. The curative effect, bone mineral density, bone pain [visual analogue scale (VAS)], bone metabolism index [procollagen type Ⅰ N-terminal propeptide (PINP), type I C-terminal cross linked peptide (CTX-1), serum oxidative stress markers [malondialdehyde (MDA) and advanced oxidation protein product (AOPP)]before and after treatment were compared between the two groups. Results: After treatment, the total effective rate of the observation group was higher than that of the control group (97.50% vs 82.50%, P<0.05), and the VAS score was lower than that of the control group (P<0.05). After treatment, PINP increased in the two groups (P<0.05), and it was higher in the observation group (P<0.05). The levels of CTX-1 in the two groups were decreased (P<0.05), and the level in the observation group was lower (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: Yishen Bugan Jiangu Decoction combined with western medicine is effective in the treatment of primary osteoporosis, which can improve bone metabolism and oxidative stress.
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