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Explore the Mechanism of Quercetin in the Treatment of Alzheimer's  Disease by Network Pharmacology and Molecular Docking WANG Tian-xu, LIU Ci, CUI Yong-yuan, ZHANG Xin, WU Miao-miao, SHEN Li-xia ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 31-.   DOI: 10.3969/j.issn.2095-1396.2024.02.006 Abstract （156）      PDF（pc） （4082KB）（78）       Knowledge map    Save Objective: To investigate the mechanism of quercetin in the treatment of Alzheimer's disease based on network pharmacology and molecular docking. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, PubChem database and Swiss Target Prediction platform were used to predict potential targets of quercetin. Potential targets for Alzheimer's disease were obtained through the Disgenet database. Common targets were obtained by intersection of potential quercetin targets and potential Alzheimer's disease targets through Venn diagram. The common targets were imported into DAVID database for screening at P<0.05, and gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed. The protein-protein interaction (PPI) network was constructed utilizing STRING database. The centiscape 2.2 plug-in of Cytoscape 3.6.0 software was used to analyze the PPI network, and the key target of quercetin in the treatment of Alzheimer’s disease was obtained. The top 5 key targets will be selected as the core targets in order of degree from largest to smallest, and the selected core targets will be subjected to molecular docking with SailVina final software. Results: There were 319 potential targets of quercetin in the three databases, 673 potential targets of Alzheimer's disease, 92 intersection targets, and 23 key targets screened out. The biological processes in GO analysis mainly included positive regulation of gene expression, positive regulation of apoptosis, response to exogenous stimuli, and negative regulation of apoptosis. KEGG pathway analysis mainly concentrated in IL-17, HIF-1, FoxO, TNF, PI3K-Akt, MAPK signaling pathway and so on. The average docking affinity between quercetin and core target molecules was -7.92 kcal·mol-1. Quercetin has good binding activity with the core targets IL-6, AKT, TP53, TNF and IL-1β. Conclusion: The potential targets and signaling pathways of quercetin in the treatment of AD have been found by network pharmacology and molecular docking techniques, which provided theoretical basis for subsequent experimental studies. Related Articles | Metrics | Comments（0）

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Research Progress on Mitochondrial Metabolomics and Its Application in Research of Traditional Chinese Medicine HUANG Xing-yue, QIN Xue-mei, LIU Yue-tao ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 59-.   DOI: 10.3969/j.issn.2095-1396.2024.03.008 Abstract （150）      PDF（pc） （919KB）（91）       Knowledge map    Save Mitochondria is a kind of semi-autonomous organelle, which integrates three basic life activities: material metabolism, energy metabolism and genetic variation, and its metabolism is at the core of cell metabolic network. Current metabonomics mostly focuses on the metabolic characteristics of the whole cell, but lacks the specific reflection of mitochondrial metabolism. Therefore, it is necessary to study the metabolism of mitochondria. Traditional Chinese medicine has unique advantages and characteristics in treating diseases based on syndrome differentiation. Mitochondrial metabolism is also one of its important targets. The study of traditional Chinese medicine based on mitochondrial metabonomics is helpful to reveal the mechanism and target of traditional Chinese medicine in energy metabolism network from the perspective of mitochondrial metabolism. At present, there are many problems and difficulties in mitochondrial metabonomics research, such as the extraction, separation and evaluation of mitochondria, metabolites and so on. This paper summarizes the present situation of mitochondrial metabonomics and its research progress in the field of traditional Chinese medicine, which provides technical support and new research direction for the scientific research of traditional Chinese medicine. Related Articles | Metrics | Comments（0）

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Research Progress of Curcumin Nano-Preparations YUAN Xin-ru, ZHANG Dan-shen ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 54-.   DOI: 10.3969/j.issn.2095-1396.2024.02.009 Abstract （148）      PDF（pc） （1011KB）（98）       Knowledge map    Save Curcumin (Cur) is a diketone compound extracted from the rhizomes of the genus turmeric, which has a wide range of pharmacological activities such as antioxidant, anti-inflammatory, anti-tumor, and neuroprotection, but has low solubility, short half-life, low bioavailability, difficult to penetrate the blood-brain barrier and other shortcomings limit its clinical application. In recent years, it has been prepared into liposomes, solid dispersions, polymer nanoparticles, polymer micelles, nanosuspensions, etc., which significantly improved the solubility, bioavailability and targeting of Cur, and improved drug activity. This article reviewed the nano-preparations of Cur, aiming to provide references for its further research and development. Related Articles | Metrics | Comments（0）

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Exploring the Mechanism of Ganmai Dazao Decoction in Treating Cardiac Neurosis Based on Network Pharmacology and Molecular Docking HAO Sheng-jie, DU Yu-xin, LU Shang-kai, HUANG Shi-jie, WANG Hui, WANG Bing-jie, ZHAO Bo, LI Meng-wei, LI Qian ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 36-.   DOI: 10.3969/j.issn.2095-1396.2024.03.005 Abstract （136）      PDF（pc） （4417KB）（103）       Knowledge map    Save Objective：To investigate the mechanism of action of Ganmai Dazao Tang in the treatment of cardiac neurosis based on network pharmacology combined with molecular docking. Methods: We searched the Chinese Medicine System Pharmacology (TCMSP) database to obtain the target information of active ingredients, and mined the GeneCards database to construct disease targets. The STRING (https://string-db.org/) database was used to construct protein-protein interaction (PPI) network models, which were visualized and analyzed with Cytoscape 3.8.2. The online analysis platform DAVID v6.8 was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis and gene ontology (GO) enrichment analysis on the key targets. KEGG signaling pathway and GO enrichment analysis were mapped using R package. Molecular docking was accomplished using AutoDock Vina, Pymol 2.0.1, and Openbable 3.1.1. Results: A total of 107 active ingredients including quercetin, lignans, kaempferol, naringenin, isorhamnetin and other biologically important components acted on 203 potential targets for disease drug therapy including STAT3, JUN, TNF, TPT53, AKT1, EGFR. And it was widely enriched in a series of signaling pathways of TNF, HIF-1 and Toll-like receptor, and the core active ingredients were also tightly bound to the core targets. Conclusion: Flavonoids, triterpenoids and phenols in Ganmai Dazao Tang can stably bind to the disease targets of cardiac neurosis, which further confirmed that the formula treats functional disorders, dirty agitation, depression and anxiety through multi-components, multitargets and multi-pathways, which provides scientific basis and reference for the treatment of cardiac neurosis by traditional Chinese medicine. Related Articles | Metrics | Comments（0）

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Exploring the Active Ingredients and Mechanism of Action of Paeoniae Radix Alba in the Treatment of Epilepsy Based on Network Pharmacology Methods XIA Lei, YOU Si-han, YIN Hong-yan, HAO Jun-rong, GUO Chun-yan ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 26-.   DOI: 10.3969/j.issn.2095-1396.2024.02.005 Abstract （131）      PDF（pc） （3233KB）（57）       Knowledge map    Save Objective：To explore the active ingredients and mechanism of action of Paeoniae Radix Alba in the treatment of epilepsy through network pharmacology. Methods: Obtain and screen the active ingredients and targets of Paeoniae Radix Alba from the Encyclopedia of Traditional Chinese Medicine database; Collect epilepsy related genes in GeneCards, OMIM, and DisGeNET databases. After taking the intersection of the active ingredient targets of Paeoniae Radix Alba and epilepsy disease targets, a protein interaction network was constructed using the STRING database. Then, visualization analysis was performed using Cytoscape 3.9.1 software. Core targets were screened using the Centiscape 2.2 plugin, and gene ontology functional annotation (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the David database, Finally, use Cytoscape 3.9.1 software to construct a drug component target network diagram. Results: There were 158 active ingredient targets in Paeoniae Radix Alba, 2398 disease targets in epilepsy, 38 intersection genes between Paeoniae Radix Alba and epilepsy, and GO enrichment analysis yielded BP136 entries, CC41 entries, MF49 entries. KEGG analysis revealed 106 pathways. Conclusion: Paeoniae Radix Alba may act on 38 targets such as TNF, Akt1, ALB, and HSP90AA1, inhibiting signaling pathways such as AGE-RAGE and NF kappa B, thereby treating epilepsy in terms of anti-inflammatory effects. Related Articles | Metrics | Comments（0）

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Research Progress on the Correlation Between Heart Failure and Insulin Resistance ZHANG Ze-peng, ZHANG Ai-ai, YANG Bo, LU Meng, LI Fang-jiang ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 59-.   DOI: 10.3969/j.issn.2095-1396.2024.02.010 Abstract （122）      PDF（pc） （1023KB）（60）       Knowledge map    Save In recent years, the treatment of cardiovascular diseases such as hypertension and coronary heart disease has made remarkable progress. However, the incidence of heart failure (HF) remains high and has become an important challenge in the cardiovascular field. At present, the urgent problem is how to effectively delay the occurrence and development of HF and reduce its fatality rate. Recent studies have shown that insulin resistance (IR) is closely related to HF. This article summarized the current research status of the correlation between heart failure and insulin resistance, and provided new ideas and strategies for the treatment of heart failure. The review is now reported as follows. Related Articles | Metrics | Comments（0）

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Exploration of the Effect of Danggui-Shaoyao-Sanon Steroid Hormone Receptor in the Treatment of Diabetic Cognitive Impairment Based on Network Pharmacology and Molecular Docking LI Tian-tian, FU Xin, YE Jing-ying ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 17-.   DOI: 10.3969/j.issn.2095-1396.2024.03.003 Abstract （107）      PDF（pc） （4825KB）（104）       Knowledge map    Save Objective： Using network pharmacology and molecular docking technology explores the effect of danggui-shaoyao-san (DSS) on steroid hormone receptors to treat diabetic cognitive impairment. Methods: Angelica sinensis, Paeonia lactiflora Pall, Atractylodes macrocephala, Ligusticum chuanxiong Hort, Poria cocos, and Alisma Plantago-aquatica Linn in DSS were selected as the research objects, and the main chemical components and corresponding targets of six traditional Chinese medicines were screened by TCMSP database. Gene targets related to diabetic cognitive impairment were retrieved from OMIM database and Gene Cards database. Steroid hormone receptors retrieved from the literature and NCBI database and potential targets of DSS in the treatment of diabetic cognitive impairment were input into the STRING platform to obtain the protein network interaction map. The 3D structure of the compound was obtained from the PubChem database, and the 3D structure of the steroid receptor was obtained from the PDB database. The molecular docking software AutoDock4.2.6 was used to obtain the binding energy of the active component of DSS and the steroid hormone receptors. Results: 47 active components and 161 targets of DSS were obtained; 8 018 disease targets of diabetic cognitive impairment; 138 potential targets of DSS in the treatment of diabetic cognitive impairment; 11 steroid hormone receptors. Base on the analysis of protein interaction network, ER α, GR, AR, PR and ER β were a core target. Finally, molecular docking was carried out to screen the results. Conclusion: Based on the research method of network pharmacology and molecular docking, catechin, diosgenin, naringenin, quercetin, taxifolin, kaempferol are mainly found in DSS. These active ingredients act on steroid hormone receptors such as ER α, GR, AR, PR and ER β. Related Articles | Metrics | Comments（0）

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Mechanism of Dandelion's Anti-Gastric Cancer Effects Based on Network Pharmacology and Molecular Docking Analysis WANG Jing, LI Lu-lu, WANG Shao-xuan, TAO Zi-ai, JIA Gui-yan, DONG Shang-lin ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 27-.   DOI: 10.3969/j.issn.2095-1396.2024.03.004 Abstract （105）      PDF（pc） （4983KB）（17）       Knowledge map    Save Objective：To predict the active components and primary targets of dandelion in the treatment of gastric cancer using network pharmacology, and to explore its underlying mechanisms. Methods: Potential active components of dandelion were retrieved from TCMID, PubChem, and Swiss Target Prediction databases, and their corresponding targets were predicted. Gastric cancer-related targets were identified using OMIM, GeneCards and DrugBank databases. Common targets between dandelion and gastric cancer were identified. STRING database was used for protein-protein interaction analysis. Cytoscape 3.9.1 was employed to create a "drug-componenttarget- disease" network, while Network Analyzer was utilized to analyze the key targets. GO and KEGG enrichment analyses were conducted using Bioconductor packages in R software Molecular docking of dandelion's main active components with core targets was conducted using AutoDock. Results: 65 potential active components and 577 drug targets of dandelion were identified, along with 1 517 gastric cancer targets, yielding 118 common targets. Key active components include artemetin, quercetin, luteolin, myricetin, hesperetin, coniferyl aldehyde, and esculetin. Key targets include STAT3, SRC, MAPK3, HSP90AA1, PIK3R1, MAPK1, and PIK3CA. GO analysis identified 2 402 biological processes, 91 cellular components, and 168 molecular functions. KEGG analysis identified 163 pathways. Molecular docking indicated strong binding affinities between major active components and key targets. Conclusion: Dandelion exhibits therapeutic effects on gastric cancer through multiple components, pathways, and targets, providing a theoretical basis for further investigation into its antigastric cancer mechanisms and offering relevant evidence for subsequent experimental validation. Related Articles | Metrics | Comments（0）

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Jiaotai Pill D rug-Containing Serum Ameliorates the Injury of SH-SY5Y Cells Induced by High Glucose/Palmitic Acid by Activating SIRT1 and Inhibiting Endoplasmic Reticulum Stress LI Pei-sen ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 7-.   DOI: 10.3969/j.issn.2095-1396.2024.02.002 Abstract （105）      PDF（pc） （3278KB）（164）       Knowledge map    Save Objective： To investigate the effect of Jiaotai Pill on SH-SY5Y cell injury induced by high glucose and high lipid. Methods： Twenty Sprague Dawley rats were randomly divided into control group and Jiaotai Pill group, with 10 rats in each group. The rats in the Jiaotai Pill group were given Jiaotai Pill solution 8.4 g·kg-1 by gavage, and the rats in the control group were given normal saline by gavage for 7 consecutive days to prepare blank serum and Jiaotai Pill serum. SH-SY5Y cells were cultured in vitro and treated with different proportions of serum for 24 hours. CCK-8 assay was used to detect the effect of serum containing Jiaotai Pill on the proliferation of SH-SY5Y cells. The cells were and divided into control group, low dose group and high dose group, SIRT1 inhibitor EX527 group according to the proportion of drug-containing serum and cultured with 25 mmol·L-1 high glucose and 200 μmol·L-1 palmitic acid or normal medium. Endoplasmic reticulum (ER) associated proteins [inositol-requiring enzyme 1α(IRE1α), phospho-inositol-requiring enzyme 1α(p-IRE1α), protein kinase R-like endoplasmic reticulum kinase (PERK), phospho-protein kinase R-like endoplasmic reticulum kinase (p-PERK), activating transcription factor 6(ATF6) and glucose regulated protein 78 (GRP78)、protein disulfide isomerase（PDI）] were detected by Western blot. Immunofluorescence was used to detect the expression of energy metabolism-related proteins (SIRT1、p-IRE1α、 p-PERK) in each group. Results： Compared with the control group, GRP78, the ratio of p-PERK to PERK and the ratio of p-IRE1α to IRE1α were significantly increased in the model group (P<0.05), SIRT1 and PDI were significantly decreased (P<0.05). Compared with the model group, the expressions of SIRT1 and PDI were up-regulated (P<0.05), and the expressions of GRP78, p-PERK/PERK,and p-IRE1α/IRE1α were down-regulated (P<0.05) in a concentrationdependent way in Jiaotai Pill low and high concentration groups.EX527 group significantly reversed the effect of Jiaotai pill(P<0.05). Conclusion： The drug-containing serum of Jiaotai Pill improve the high glucose/palmitic acid induced injury of SH-SY5Y cells by improving SIRT1 and inhibiting endoplasmic reticulum stress. Related Articles | Metrics | Comments（0）

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Research on the Weight Loss Function and Mechanism of Tuckahoe Sawa DiarrheaTablets Based on Network Pharmacology and Molecular Docking Technology GAO Xiao-xin, CAO Chun-ran, LI Xue-mei ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 14-.   DOI: 10.3969/j.issn.2095-1396.2024.02.003 Abstract （104）      PDF（pc） （2472KB）（51）       Knowledge map    Save Objective：Network pharmacology and molecular docking technology combined with TCM dialectical formula were used to explore the weight loss effect and mechanism of tuckahoe sawa diarrhea tablets. Methods： According to the TCM syndrome type and medication experience, the active ingredients and targets of Poringze laxative tablets were collected through TCMSP database and UniProt database. DrugBank, OMIM database and GeneCards database were used to obtain obesity-related targets; The use of venn diagram to obtain the target of poria laxative tablets in the treatment of obesity; The STRING database constructs the target protein interaction network; Using Cytoscape 3.7.2 software to build a "drug-ingredient-key target" network; KEGG enrichment analysis of key targets was carried out by Metescape database. Finally use PyMOL 2.2 and Autodock Tools 1. 5. 6 software to carry out molecular docking of core targets and core components. Results： After topological analysis, a total of 52 key targets were obtained, and PTGS2 and PPARG may be important targets for the treatment of obesity by poria. In KEGG analysis, Insulin resistance, HIF-1 signaling pathway, PI3K-Akt signaling pathway, and Adipocytokine signaling pathway were closely related to the treatment of obesity. Molecular docking showed good results of quercetin docking with PTGS2 and PPARG, indicating that quercetin may be a key component of tuckahoe sawa diarrhea tablets in the treatment of obesity. Conclusion： tuckahoe sawa diarrhea tablets have the function of slimming in spleen deficiency and obstructive obesity, and realize the function of weight loss through multiple targets and multiple pathways. Related Articles | Metrics | Comments（0）

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Study on Ultra High Performance Liquid Chromatography Fingerprint of Mistletoe ZHANG Ao-kai, LI Wen-jie, DONG Yang-yang, CHU Han-bing, DONG Xue-hai, ZHAO Li-yan, ZHANG Wanming , ZHANG Yuan-yuan ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 1-.   DOI: 10.3969/j.issn.2095-1396.2024.03.001 Abstract （100）      PDF（pc） （1586KB）（76）       Knowledge map    Save Objective：The UPLC fingerprint of Mistletoe was established and the similarity evaluation was carried out. Combined with cluster analysis and principal component analysis, chemometric analysis methods were used to provide reference for the quality consistency evaluation of Mistletoe medicinal materials. Methods: The chromatographic column was Waters ACQUITY UPLC HSS T3 chromatographic column (2.1×100 mm, 1.8 μm). The mobile phase was acetonitrile ( A ) -0.5 % acetic acid water ( B ) with gradient elution. The detection wavelength was 254 nm. Column temperature 30℃ ; the injection volume was 3 μL.《The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine ( 2012 Edition )》was used to establish the fingerprint of Mistletoe, and the common peaks were calibrated and the similarity was evaluated. Cluster analysis and principal component analysis were performed on 14 batches of Mistletoe by SPSS 26.0. Results: The methodological investigation results of the fingerprint of Mistletoe were good, and 14 common peaks were identified. By comparing with the reference substance, it was identified that the No.4 peak was Syringin and the No.9 peak was homoeriodictyol-7-O-β-D-glucoside. The similarity evaluation results showed that the similarity of 11 batches of samples was above 0.950, and the similarity of the other 3 batches was less than 0.950. The samples were clustered into three categories by cluster analysis, S5 was a separate category, S12, S7, S9 were a category, and the rest of the samples were clustered into one category. The cumulative variance contribution rate of the four principal component factors obtained by principal component analysis was 88.732%. The principal component factors of S5, S10, S3 and S11 samples were in the top four of the comprehensive scores, and the overall quality was good. Conclusion: The fingerprint method established in this study has good separation, precision and stability, short analysis time and strong specificity. Combined with similarity evaluation, cluster analysis and principal component analysis, it can provide a theoretical basis for the quality control of Mistletoe medicinal materials. Related Articles | Metrics | Comments（0）

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Mining of the Compatibility and Application Characteristics of Chinese Medicine Nidus Vespae Based on the Pharmacy Intelligence Network Database ZHANG Feng, ZHONG Juan, PAN Wen-bo ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 20-.   DOI: 10.3969/j.issn.2095-1396.2024.02.004 Abstract （94）      PDF（pc） （2704KB）（130）       Knowledge map    Save Objective: Based on data mining, the compatibility and application characteristics of formulas containing Nidus Vespae were discussed to provide references for rational drug use in honeycombs. Methods: Collect the prescriptions of Nidus Vespae in the Pharmacy Intelligence network database, construct Nidus Vespae prescription database, Microsoft Office Excel 2019, IBM SPSS Modeler 18.0, and IBM SPSS Statistics 23.0 were used to analyze the properties, flavors, Meridian Tropism, efficacy, and indications of Chinese herbal medicines. Association rules and cluster analysis were also conducted for high-frequency Chinese herbal medicines. Results: Among the 147 prescriptions that met the inclusion requirements, 353 Chinese herbs were used. Among the 1 597 cumulative uses, the most commonly used medicines were anti-toxin, insecticides, antipruritic drugs, heat-clearing drugs and drugs for promoting blood circulation and removing blood stasis, the medicine taste is more sweet, bitter and pungent, and mainly belongs to liver, stomach, spleen and heart meridian. The support and confidence of the 29 Association rules of 21 high-frequency Chinese herbal medicines were “Nidus Vespae-snake shed”,“ Nidus Vespae-angelica” and“ Nidus Vespae-pangolin”. the prescriptions of Nidus Vespae were mainly used to treat carbuncle, toothache, hemorrhoid, rheumatism and other diseases, 19 kinds of high-frequency Chinese herbs and 4 kinds of New Prescriptions were obtained by analyzing the prescriptions of treating carbuncle. Conclusion: In this study, data mining was used to analyze the compatibility characteristics and composition rules of formulas containing Nidus Vespae, which can provide references for clinical application and new drug development of Nidus Vespae. Related Articles | Metrics | Comments（0）

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Research Progress of TRIM32 and Related Nervous System Diseases SUN Xiao-jing, MENG Xian-yong, YU Hui-jiao, DONG Xiao-hua ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 47-.   DOI: 10.3969/j.issn.2095-1396.2024.02.008 Abstract （94）      PDF（pc） （1032KB）（76）       Knowledge map    Save Tripartite motif proteins (TRIM) are a class of proteins involved in a wide range of biological processes, including cell growth and differentiation, development, apoptosis, immune response, and tumorigenesis. Among them, TRIM32 is an E3 ubiquitin ligase that belongs to the TRIM protein family. Although there are few studies on TRIM32, its role in maintaining the physiological function of the nervous system is extremely important. Therefore, this review aims to review the role of TRIM32 in neurological diseases. Related Articles | Metrics | Comments（0）

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Xingnaojing Improves Neuroinflammation and Cognitive Dysfunction in Heatstroke-Induced Rats Based on TLR4 / NF-κB Signaling P athway ZHENG Li-li, LIU Mei-cen ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 1-.   DOI: 10.3969/j.issn.2095-1396.2024.02.001 Abstract （92）      PDF（pc） （2223KB）（45）       Knowledge map    Save Objective：To observe the effect of Xingnaojing on inflammatory cytokines in brain tissues in heatstroke-induced rats, and to explore the mechanism of cognitive improvement. Methods: Thirty-six Wistar rats were randomly divided into control group, model group, and Xingnaojing group. The rats were intervened with high temperature of (41±0.5) ℃ and high humidity of (75%±5%). The core temperature of the rats was monitored, when the temperature reach to 42 ℃ , the rats were treated with cooling administration, and the Xingnaojing group were administrated with drugs i.v. After 3 days of continuous intervention, water maze tests were performed, and then brains were taken for the expression of inflammatory cytokines and related proteins dectection. Results: The results of the study showed that Xingnaojing had a significant protective effect on cognitive dysfunction in heatstroke-induced rats. The intervention of Xingnaojing significantly reduced the expression of inflammatory cytokines in the rat brain,inhibited the activation of microglia in the brain, and improved neuronal apoptosis in the hippocampal CA1 area. In addition, Xingnaojing significantly reversed the increased expression of TLR4 protein, increased NF-κB phosphorylation and increased NLRP3 protein expression in the rat brain caused by heatstroke. Conclusion: Xingnaojing can improve cognitive dysfunction by suppressing the inflammatory response in the brain of heatstroke disease. Related Articles | Metrics | Comments（0）

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Optimization of Extraction Process of Total Flavonoids from Green Pepper Leaves and Its Antioxidant Activity LAN Zhi-fu ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 9-.   DOI: 10.3969/j.issn.2095-1396.2024.03.002 Abstract （90）      PDF（pc） （4034KB）（58）       Knowledge map    Save Objective: The purpose was to explore the technology of snail enzyme-assisted extraction of total flavonoids from green pepper leaves and its antioxidant activity. Methods: Box-Behnken response surface method was introduced on the basis of single factor experiment, and the snail enzyme-assisted extraction process of total flavonoids from green pepper leaves was optimized, and a quadric quadratic regression equation was established, and its antioxidant activity was investigated by its scavenging rate of DPPH and OH free radicals. Results: The experimental results showed that the total flavonoids from green pepper leaves could be extracted efficiently under the optimum conditions of enzymolysis time 61 min, enzyme dosage 1.8%, pH 5.5 and enzymolysis temperature 57℃ , and the extraction rate reached the highest, reaching 26.49 mg·g-1. Comparing the actual value with the predicted value (26.59 mg·g-1), the relative error was only 0.38%, which proved that the model had high accuracy and reliability. The free radical scavenging effect showed that the semi-inhibitory concentrations (IC50) of total flavonoids from green pepper leaves on DPPH and OH radicals were 69.11 mg·L-1 and 163.31 mg·L-1, respectively. Conclusion: Response surface methodology can be used to optimize the extraction process of total flavonoids from green pepper leaves, and the extracted total flavonoids from green pepper leaves showed certain antioxidant activity, which provided a theoretical basis for the development and utilization of green pepper leaf resources. Related Articles | Metrics | Comments（0）

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Application of Intestinal Obstruction Catheter Combined with Enema with Chinese Herbs in Children with Non Strangulated Intestinal Obstruction SUN Chuan-wei ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 54-.   DOI: 10.3969/j.issn.2095-1396.2024.03.007 Abstract （85）      PDF（pc） （1114KB）（59）       Knowledge map    Save Objective: To explore the application of intestinal obstruction catheter combined with enema with Chinese herbs in children with non strangulated intestinal obstruction. Methods: 100 children with non strangulated intestinal obstruction admitted to our hospital from July 2020 to July 2022 were selected as the study subjects. Randomly divided into general enema group (n=50) and Magnolia officinalis exhaust group (n=50), The general enema group was treated with warm saline enema and soapy water enema; The Houpu exhaust group was treated with intestinal obstruction catheter and Houpu exhaust mixture enema. The changes of serum inflammatory factors before and after treatment were analyzed; The time of relieving abdominal distension, time of spontaneous exhaust, time of resuming diet and time of disappearance of gas liquid level were collected in the two groups; The clinical treatment efficiency of the two groups was analyzed. Results: The relieving time of abdominal distension, the time of spontaneous exhaust, the time of resuming diet and the time of disappearance of gas liquid level in the exhaust group of Magnolia officinalis were shorter than those in the general enema group (P<0.05). The levels of IFABP, D-D, PLR and PCT in the exhaust group were lower than those in the general enema group (P<0.05). The clinical cure rate of Magnolia officinalis exhaust group was higher than that of general enema group (P<0.05). Conclusion: Intestinal obstruction catheter combined with traditional Chinese medicine enema of Magnolia officinalis can effectively shorten the time of abdominal distension, exhaust and diet recovery in children with non strangulated intestinal obstruction, reduce inflammatory reaction, and have a definite clinical effect. Related Articles | Metrics | Comments（0）

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Efficacy and Safety Analysis of Recombinant Human Growth Hormone Therapy in Children with Idiopathic Short Stature with Different Body Mass Indexes ZHANG Wen-hui, XU Zheng-rong ACTA NEUROPHARMACOLOGICA    2024, 14 (3): 47-.   DOI: 10.3969/j.issn.2095-1396.2024.03.006 Abstract （83）      PDF（pc） （1109KB）（40）       Knowledge map    Save Objective：This article aims to analyze the differences in efficacy and safety of recombinant human growth hormone (rhGH) treatment in children with idiopathic short stature (ISS) with different body mass indexes (BMI). Methods: ISS patients who received rhGH treatment at the First Affiliated Hospital of Hebei North University were selected as the research subjects. According to the growth standards for children under 7 years old and the gender age specific body mass index (BMI) overweight and obesity thresholds for Chinese school aged children and adolescents aged 6~18, ISS patients were divided into normal BMI group, overweight group and obese group. The height (Ht), body mass index (BMI), growth velocity (GV), height standard deviation score (HtSDS), insulin like growth factor-1 (IGF-1), insulin like growth factor binding-protein-3 (IGFBP-3), fasting blood glucose (FBG), thyroid function and liver function of three groups were measured before treatment, 3 months after treatment, and 6 months after treatment. Results: After rhGH treatment, the levels of Ht, GV, HtSDS, IGF-1, and IGFBP-3 in the three groups of ISS patients increased compared to before treatment (P<0.05). The amount of increase in Ht, GV, and HtSDS in children with normal BMI were significantly higher than those in the overweight and obese groups (P<0.05), while there was no significant difference between the overweight group and obese group (P>0.05). After 6 months of treatment, the BMI of children with normal BMI increased compared to before treatment (P<0.05), while the BMI of overweight group and obese group decreased compared to before (P<0.05). rhGH treatment has no significant effect on fasting blood glucose, thyroid function and liver function. Conclusion: rhGH can significantly increase the levels of Ht, HtSDS, GV, IGF-1, and IGFBP-3 in children with ISS. The therapeutic effect of ISS patients with normal BMI is significantly better than that of overweight group and obese group. There is no significant difference in the therapeutic effect between overweight and obese ISS patients, and rhGH treatment has great safety. Related Articles | Metrics | Comments（0）

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Comparative Stud y of HER 2, RKIP and ERK Ex pression in Adenocarcinoma of Es ophagogastric J unction and D istal Gastric Adenocarcinoma FU Yu-tong, LI Hui-min, DONG Xin-yi, DU Shi-xuan, LOU Lei, CUI Jin-feng, WANG Juan, ZHANG Qing, SU Ling-rui, LI Yue-hong ACTA NEUROPHARMACOLOGICA    2024, 14 (2): 38-.   DOI: 10.3969/j.issn.2095-1396.2024.02.007 Abstract （76）      PDF（pc） （4080KB）（51）       Knowledge map    Save Objective: To compare the expression of HER2, RKIP, and ERK in adenocarcinoma of esophagogastric junction (AEG) and distal gastric adenocarcinoma(DGA), and explore the differences in the pathogenesis of AEG and DGA. Methods: The expression of HER2, RKIP and ERK in AEG, DGA and normal gastric mucosal tissue were detected by immunohistochemistry, and the relationship between HER2, RKIP, and ERK with clinical pathological characteristics and their correlation were analyzed. Results: The positive expression rate of HER2 in AEG was higher than that in DGA, and the difference was statistically significant (P<0.05). The positive expression rates of RKIP in AEG and DGA were lower than that in normal gastric mucosal tissue, while the positive expression rates of ERK were higher than that in normal gastric mucosal tissue (P<0.05). In AEG, the positive expression rate of RKIP was lower in the high/moderate differentiation group and lymph node metastasis group, while the positive expression rate of ERK was higher in the low differentiation group, serosa invasion group, and lymph node metastasis group (P<0.05); HER2 expression was positively correlated with ERK expression (r=0.352, P=0.007), while RKIP expression was negatively correlated with ERK expression (r=-0.521, P=0.000).In DGA, the positive expression rate of RKIP was lower in the poorly differentiated group and lymph node metastatic group, while the positive expression rate of ERK was higher in the lymph node metastatic group (P<0.05); RKIP expression was negatively correlated with ERK expression (r=-0.287, P=0.021). Conclusion: The positive expression rate of HER2 in AEG is higher than that in DGA, and the high expression of ERK is more closely related to the clinicopathological characteristics of AEG patients. The expression of RKIP is closely related to the differentiation degree and lymph node metastasis of AEG and DGA. The expression of HER2, RKIP, and ERK in AEG and DGA is different, indicating that the regulatory mechanism of MAPK signaling pathway is not completely consistent in different locations of gastric adenocarcinoma. Related Articles | Metrics | Comments（0）

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Effects of Tau Protein on Mitochondrial Function in Alzheimer's Disease YAO Si-fan, ZHANG Xin, SHEN Li-xia ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 54-.   DOI: 10.3969/j.issn.2095-1396.2024.04.010 Abstract （66）      PDF（pc） （1084KB）（6）       Knowledge map    Save Tau protein is an important protein that physiologically promotes microtubule assembly and stabilization and is involved in neuronal development, axonal transport and neuronal polarity. In Alzheimer's disease (AD), Tau proteins undergo pathological modifications in which soluble Tau assembles into insoluble filaments, leading to synaptic failure and neurodegenerative lesions. Mitochondria are the most important organelles in neurons and are the main source of energy, providing adenosine triphosphate (ATP) through oxidative phosphorylation to maintain normal neuronal homeostasis and function. A growing body of evidence suggests that mitochondrial dysfunction plays a key role in the pathogenesis of AD. Negative effects on mitochondrial bioenergetics, transport and morphology in neurons lead to synaptic damage and cognitive decline in AD. Tau proteins have been shown to interact with mitochondrial proteins, impairing mitochondrial dynamics, mitochondrial morphology and autophagy, leading to neurotoxicity. This article provides a review of the effects of Tau proteins on mitochondria in AD and the mechanisms of action. Related Articles | Metrics | Comments（0）

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Research Progress on the Etiology of Febrile Seizures and the Treatment HAO Xue-nan, YANG Lei, ZHAO Wen-ya, ZHANG Wei ACTA NEUROPHARMACOLOGICA    2024, 14 (4): 42-.   DOI: 10.3969/j.issn.2095-1396.2024.04.008 Abstract （62）      PDF（pc） （1059KB）（10）       Knowledge map    Save Febrile seizures (FS) are convulsions characterized by a body temperature of 38.5℃ or higher, without central nervous system infection or other epileptic causes, affecting 2% to 14% of the global population and being a common neurological disorder in childhood. The occurrence of FS is age dependent, most prevalent in children aged 6 months to 5 years. The pathogenesis of FS remains unclear, although it is known that various factors can induce FS, including abnormal body temperature, age and brain maturity, genetic factors, and infections. Moreover, other factors can predispose to the development of FS. Current treatment of FS primarily involves antiepileptic drugs. This article reviews the pathophysiological mechanisms of FS, with a focus on the research progress of FS etiology and treatment. Related Articles | Metrics | Comments（0）