Objective：The UPLC fingerprint of Mistletoe was established and the similarity evaluation was carried out. Combined with cluster analysis and principal component analysis, chemometric analysis methods were used to provide reference for the quality consistency evaluation of Mistletoe medicinal materials. Methods: The chromatographic column was Waters ACQUITY UPLC HSS T3 chromatographic column (2.1×100 mm, 1.8 μm). The mobile phase was acetonitrile ( A ) -0.5 % acetic acid water ( B ) with gradient elution. The detection wavelength was 254 nm. Column temperature 30℃ ; the injection volume was 3 μL.《The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine ( 2012 Edition )》was used to establish the fingerprint of Mistletoe, and the common peaks were calibrated and the similarity was evaluated. Cluster analysis and principal component analysis were performed on 14 batches of Mistletoe by SPSS 26.0. Results: The methodological investigation results of the fingerprint of Mistletoe were good, and 14 common peaks were identified. By comparing with the reference substance, it was identified that the No.4 peak was Syringin and the No.9 peak was homoeriodictyol-7-O-β-D-glucoside. The similarity evaluation results showed that the similarity of 11 batches of samples was above 0.950, and the similarity of the other 3 batches was less than 0.950. The samples were clustered into three categories by cluster analysis, S5 was a separate category, S12, S7, S9 were a category, and the rest of the samples were clustered into one category. The cumulative variance contribution rate of the four principal component factors obtained by principal component analysis was 88.732%. The principal component factors of S5, S10, S3 and S11 samples were in the top four of the comprehensive scores, and the overall quality was good. Conclusion: The fingerprint method established in this study has good separation, precision and stability, short analysis time and strong specificity. Combined with similarity evaluation, cluster analysis and principal component analysis, it can provide a theoretical basis for the quality control of Mistletoe medicinal materials.

Objective: The purpose was to explore the technology of snail enzyme-assisted extraction of total flavonoids from green pepper leaves and its antioxidant activity. Methods: Box-Behnken response surface method was introduced on the basis of single factor experiment, and the snail enzyme-assisted extraction process of total flavonoids from green pepper leaves was optimized, and a quadric quadratic regression equation was established, and its antioxidant activity was investigated by its scavenging rate of DPPH and OH free radicals. Results: The experimental results showed that the total flavonoids from green pepper leaves could be extracted efficiently under the optimum conditions of enzymolysis time 61 min, enzyme dosage 1.8%, pH 5.5 and enzymolysis temperature 57℃ , and the extraction rate reached the highest, reaching 26.49 mg·g^-1. Comparing the actual value with the predicted value (26.59 mg·g^-1), the relative error was only 0.38%, which proved that the model had high accuracy and reliability. The free radical scavenging effect showed that the semi-inhibitory concentrations (IC50) of total flavonoids from green pepper leaves on DPPH and OH radicals were 69.11 mg·L^-1 and 163.31 mg·L^-1, respectively. Conclusion: Response surface methodology can be used to optimize the extraction process of total flavonoids from green pepper leaves, and the extracted total flavonoids from green pepper leaves showed certain antioxidant activity, which provided a theoretical basis for the development and utilization of green pepper leaf resources.

Objective： Using network pharmacology and molecular docking technology explores the effect of danggui-shaoyao-san (DSS) on steroid hormone receptors to treat diabetic cognitive impairment. Methods: Angelica sinensis, Paeonia lactiflora Pall, Atractylodes macrocephala, Ligusticum chuanxiong Hort, Poria cocos, and Alisma Plantago-aquatica Linn in DSS were selected as the research objects, and the main chemical components and corresponding targets of six traditional Chinese medicines were screened by TCMSP database. Gene targets related to diabetic cognitive impairment were retrieved from OMIM database and Gene Cards database. Steroid hormone receptors retrieved from the literature and NCBI database and potential targets of DSS in the treatment of diabetic cognitive impairment were input into the STRING platform to obtain the protein network interaction map. The 3D structure of the compound was obtained from the PubChem database, and the 3D structure of the steroid receptor was obtained from the PDB database. The molecular docking software AutoDock4.2.6 was used to obtain the binding energy of the active component of DSS and the steroid hormone receptors. Results: 47 active components and 161 targets of DSS were obtained; 8 018 disease targets of diabetic cognitive impairment; 138 potential targets of DSS in the treatment of diabetic cognitive impairment; 11 steroid hormone receptors. Base on the analysis of protein interaction network, ER α, GR, AR, PR and ER β were a core target. Finally, molecular docking was carried out to screen the results. Conclusion: Based on the research method of network pharmacology and molecular docking, catechin, diosgenin, naringenin, quercetin, taxifolin, kaempferol are mainly found in DSS. These active ingredients act on steroid hormone receptors such as ER α, GR, AR, PR and ER β.

Objective：To predict the active components and primary targets of dandelion in the treatment of gastric cancer using network pharmacology, and to explore its underlying mechanisms. Methods: Potential active components of dandelion were retrieved from TCMID, PubChem, and Swiss Target Prediction databases, and their corresponding targets were predicted. Gastric cancer-related targets were identified using OMIM, GeneCards and DrugBank databases. Common targets between dandelion and gastric cancer were identified. STRING database was used for protein-protein interaction analysis. Cytoscape 3.9.1 was employed to create a "drug-componenttarget- disease" network, while Network Analyzer was utilized to analyze the key targets. GO and KEGG enrichment analyses were conducted using Bioconductor packages in R software Molecular docking of dandelion's main active components with core targets was conducted using AutoDock. Results: 65 potential active components and 577 drug targets of dandelion were identified, along with 1 517 gastric cancer targets, yielding 118 common targets. Key active components include artemetin, quercetin, luteolin, myricetin, hesperetin, coniferyl aldehyde, and esculetin. Key targets include STAT3, SRC, MAPK3, HSP90AA1, PIK3R1, MAPK1, and PIK3CA. GO analysis identified 2 402 biological processes, 91 cellular components, and 168 molecular functions. KEGG analysis identified 163 pathways. Molecular docking indicated strong binding affinities between major active components and key targets. Conclusion: Dandelion exhibits therapeutic effects on gastric cancer through multiple components, pathways, and targets, providing a theoretical basis for further investigation into its antigastric cancer mechanisms and offering relevant evidence for subsequent experimental validation.

Objective：To investigate the mechanism of action of Ganmai Dazao Tang in the treatment of cardiac neurosis based on network pharmacology combined with molecular docking. Methods: We searched the Chinese Medicine System Pharmacology (TCMSP) database to obtain the target information of active ingredients, and mined the GeneCards database to construct disease targets. The STRING (https://string-db.org/) database was used to construct protein-protein interaction (PPI) network models, which were visualized and analyzed with Cytoscape 3.8.2. The online analysis platform DAVID v6.8 was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis and gene ontology (GO) enrichment analysis on the key targets. KEGG signaling pathway and GO enrichment analysis were mapped using R package. Molecular docking was accomplished using AutoDock Vina, Pymol 2.0.1, and Openbable 3.1.1. Results: A total of 107 active ingredients including quercetin, lignans, kaempferol, naringenin, isorhamnetin and other biologically important components acted on 203 potential targets for disease drug therapy including STAT3, JUN, TNF, TPT53, AKT1, EGFR. And it was widely enriched in a series of signaling pathways of TNF, HIF-1 and Toll-like receptor, and the core active ingredients were also tightly bound to the core targets. Conclusion: Flavonoids, triterpenoids and phenols in Ganmai Dazao Tang can stably bind to the disease targets of cardiac neurosis, which further confirmed that the formula treats functional disorders, dirty agitation, depression and anxiety through multi-components, multitargets and multi-pathways, which provides scientific basis and reference for the treatment of cardiac neurosis by traditional Chinese medicine.

Objective：This article aims to analyze the differences in efficacy and safety of recombinant human growth hormone (rhGH) treatment in children with idiopathic short stature (ISS) with different body mass indexes (BMI). Methods: ISS patients who received rhGH treatment at the First Affiliated Hospital of Hebei North University were selected as the research subjects. According to the growth standards for children under 7 years old and the gender age specific body mass index (BMI) overweight and obesity thresholds for Chinese school aged children and adolescents aged 6~18, ISS patients were divided into normal BMI group, overweight group and obese group. The height (Ht), body mass index (BMI), growth velocity (GV), height standard deviation score (HtSDS), insulin like growth factor-1 (IGF-1), insulin like growth factor binding-protein-3 (IGFBP-3), fasting blood glucose (FBG), thyroid function and liver function of three groups were measured before treatment, 3 months after treatment, and 6 months after treatment. Results: After rhGH treatment, the levels of Ht, GV, HtSDS, IGF-1, and IGFBP-3 in the three groups of ISS patients increased compared to before treatment (P<0.05). The amount of increase in Ht, GV, and HtSDS in children with normal BMI were significantly higher than those in the overweight and obese groups (P<0.05), while there was no significant difference between the overweight group and obese group (P>0.05). After 6 months of treatment, the BMI of children with normal BMI increased compared to before treatment (P<0.05), while the BMI of overweight group and obese group decreased compared to before (P<0.05). rhGH treatment has no significant effect on fasting blood glucose, thyroid function and liver function. Conclusion: rhGH can significantly increase the levels of Ht, HtSDS, GV, IGF-1, and IGFBP-3 in children with ISS. The therapeutic effect of ISS patients with normal BMI is significantly better than that of overweight group and obese group. There is no significant difference in the therapeutic effect between overweight and obese ISS patients, and rhGH treatment has great safety.

Objective: To explore the application of intestinal obstruction catheter combined with enema with Chinese herbs in children with non strangulated intestinal obstruction. Methods: 100 children with non strangulated intestinal obstruction admitted to our hospital from July 2020 to July 2022 were selected as the study subjects. Randomly divided into general enema group (n=50) and Magnolia officinalis exhaust group (n=50), The general enema group was treated with warm saline enema and soapy water enema; The Houpu exhaust group was treated with intestinal obstruction catheter and Houpu exhaust mixture enema. The changes of serum inflammatory factors before and after treatment were analyzed; The time of relieving abdominal distension, time of spontaneous exhaust, time of resuming diet and time of disappearance of gas liquid level were collected in the two groups; The clinical treatment efficiency of the two groups was analyzed. Results: The relieving time of abdominal distension, the time of spontaneous exhaust, the time of resuming diet and the time of disappearance of gas liquid level in the exhaust group of Magnolia officinalis were shorter than those in the general enema group (P<0.05). The levels of IFABP, D-D, PLR and PCT in the exhaust group were lower than those in the general enema group (P<0.05). The clinical cure rate of Magnolia officinalis exhaust group was higher than that of general enema group (P<0.05). Conclusion: Intestinal obstruction catheter combined with traditional Chinese medicine enema of Magnolia officinalis can effectively shorten the time of abdominal distension, exhaust and diet recovery in children with non strangulated intestinal obstruction, reduce inflammatory reaction, and have a definite clinical effect.

Mitochondria is a kind of semi-autonomous organelle, which integrates three basic life activities: material metabolism, energy metabolism and genetic variation, and its metabolism is at the core of cell metabolic network. Current metabonomics mostly focuses on the metabolic characteristics of the whole cell, but lacks the specific reflection of mitochondrial metabolism. Therefore, it is necessary to study the metabolism of mitochondria. Traditional Chinese medicine has unique advantages and characteristics in treating diseases based on syndrome differentiation. Mitochondrial metabolism is also one of its important targets. The study of traditional Chinese medicine based on mitochondrial metabonomics is helpful to reveal the mechanism and target of traditional Chinese medicine in energy metabolism network from the perspective of mitochondrial metabolism. At present, there are many problems and difficulties in mitochondrial metabonomics research, such as the extraction, separation and evaluation of mitochondria, metabolites and so on. This paper summarizes the present situation of mitochondrial metabonomics and its research progress in the field of traditional Chinese medicine, which provides technical support and new research direction for the scientific research of traditional Chinese medicine.