Objective：To explore the mechanism of Commelina communis L. in the treatment of febrile seizures based on network pharmacology and molecular docking technology.Methods： The effective components of Commelina communis L. and their related targets were extracted by searching the Chinese Medicine System Pharmacology (TCMSP) database and BATMAN-TCM database. The disease targets of febrile seizures were searched through GeneCards and OMIM databases. Uniport was used to convert protein and gene symbols, and Darw venn diagram platform was used to obtain venn diagram and intersection genes between drug components and diseases. A PPI network was built through the STRING database. Cytoscape3.9.1 software was used to construct the “traditional Chinese medicine-active ingredients-target” network and “key target-signaling pathway” network. The online analysis platform DAVID v6.8 was used to perform KEGG signaling pathway enrichment analysis and GO enrichment analysis of the key targets. The software R x64 4.0.2 and the drawing package ggplot2 were used to plot the KEGG signal path and GO enrichment analysis diagram. Molecular docking was done using AutoDockTools 1.5.7, Pymol 2.0.1 and Openbable 3.1.1 software. Results： There were 8 main effective components of Commelina communis L., which correspond to 140 targets, 2 210 disease targets and 64 common targets of drug diseases. 262 items were obtained by GO enrichment analysis (P<0.05), and 67 signaling pathways were selected by KEGG enrichment analysis (P<0.05). The results of molecular coupling showed that flavonoids, the main component of Commelina communis L., mainly regulating multiple signaling pathways through 3QXY, 1GFW, 2K7w and other targets to play a therapeutic role in the treatment of febrile seizures. Our research provided references and basis for the follow-up research. Conclusion： The flavonoids, β-sitosterol and monoacyl ginsenoside Rb2 in Commelina communis L. can stably bind to the target of febrile seizure disease and have stronger binding ability than phenobarbital, which is used for febrile seizures in clinical, and can treat febrile seizures through multi-component, multitarget, and multi-pathway. It provides new ideas for the research and development of Commelina communis L. and treatment options for hyperthermic seizures.

Objective：To observe the differentiation of bone marrow mesenchymal stem cells (BMSCs) induced by astragaloside A and expression of Cyclin D1. Methods: BMSCs were induced by astragaloside A with 100 mg·L-1. At l, 12, 24 h after induetion, the morphological changes of BMSCs were observed with optical microscope and the expressions of Nestin, NSE, Cyclin D1 were identified by immunocytochemical techniques. Cyclin D1 mRNA was detected by RT-PCR．Results： Bone marrow cells were primarily cultured and subcultured. The third passage of BMSCs was purified. After treatment with 100 mg·L-1 astragaloside A, some cells presented processes, were similar to neuron-like cells in morphology, and expressed Nestin and neuron-specific enolase. Nestin expression occurred earlier (P<0.05) and at a higher level (P<0.05) than expression of neuron-specific enolase. At 1 hour after treatment with astragaloside A, cyclin D1 expression was substantially down-regulated in BMSCs at both gene and protein levels. Simultaneously, BMSCs began to express nestin. No significant differences in cyclin D1 expression in BMSCs were detected between 12 and 24 hours post-treatment and the pretreatment level (P>0.05). Conclusion: Our results indicated that astragaloside A regulated cell cycle, prolonged G1/S phase transition, triggered BMSC differentiation, and finally induced BMSC differentiation into neuronal stem cells. The trophism of astragaloside A accelerated further growth of cell processes and the differentiation of cells into neurons.

Objective：To investigate the efficacy and safety of Baofukang suppository on the basis of recombinant human interferon α-2b in the treatment of high-risk human papillomavirus (HR-HPV) infection. Methods： 80 HR-HPV patients were randomly divided into 2 groups. The control group (40 cases) was treated with recombinant human interferon α-2b vaginal effervescent capsule. The observation group (40 cases) was treated with Baofukang suppository on the basis of recombinant human interferon α-2b vaginal effervescent capsule. Both groups were treated for 3 months. The treatment effects of the two groups were compared.The duration of abnormal secretions, HPV viral load, TCT test results and cervical inflammation scores of the two groups were recorded, and the adverse reactions were observed. Results： Compared with the control group (75.00%), the total effective rate of the observation group (95.00%) was higher (P<0.05). The duration of abnormal secretions in the observation group was (14.47±3.15) d, comparing with the control group (20.12±3.89) d, shows significantly shorter (P<0.05). After treatment, the normal rate of TCT in the two groups was significantly higher than that before treatment (P<0.05 ), and the normal rate of TCT in the observation group was higher than that in the control group(P<0.05). After treatment, the HPV viral load in the observation group decreased comparing with the control group (P<0.05), and the cervical inflammation score decreased (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion： Baofukang suppository based on recombinant human interferon α-2b vaginal effervescent capsule is effective and safe in the treatment of HRHPV infection.

Objective：To analyze the distribution and frequency of clopidogrel related gene CYP2C19 polymorphism in patients with acute cerebral infarction in Zhangjiakou area of Hebei province. Methods: 249 patients with acute cerebral infarction admitted to the first affiliated Hospital of Hebei North University from September 2021 to March 2023 were selected. The exfoliated cells of oral mucosa were collected and CYP2C19 genotyping was detected by POCT method. The patients were divided into groups according to sex and age, and the distribution of CYP2C19 genotypes and clopidogrel metabolic types among the groups were compared. Results: Five genotypes were detected: fast metabolic CYP2C19*1/* 1 in 9 6 cases, accounting for 38.60%; CYP2C19*1/*2 in middle metabolic type, accounting for 45.00%, 1 0 cases, accounting for 4.00%; slow metabolic type, 25 cases, accounting for 10.00%, CYP2C19*2/*3, 6 cases, accounting for 2.40%. The frequency distributions of alleles * 1, * 2 and * 3 were 63.05%, 63.05% and 3.21%, respectively. No genotypes of CYP2C19*1/*17, CYP2C19*17/*17, CYP2C19*2/*17,CYP2C19*3/*17 and CYP2C19*3/*3 were detected in this study. There was no significant difference in sex, genotype and metabolic type distribution frequency (P>0.05). There was no significant difference in age, genotype and metabolic type distribution frequency (P>0.05). Conclusion: The CYP2C19 polymorphism of clopidogrel related gene in patients with acute AIS in Zhangjiakou area of Hebei Province is mainly middle metabolic type, and the middle and slow metabolic type accounts for 61.40%. Most people have platelet hyperresponsiveness, so gene detection is carried out before medication to develop a safe, reasonable, effective and economical personalized drug treatment plan.

Objective：To investigate the therapeutic effect of sulpiride tablets combined with lurasidone tablets on schizophrenia. Methods: 80 patients with schizophrenia were randomly divided into control group (n=40) and observation group (n=40), they were treated with sulpiride tablets and sulpiride tablets combined with lurasidone tablets respectively. Both groups were treated for 8 weeks. The therapeutic effects of the two groups were evaluated. The changes of mental symptoms, cognitive function [Repeatable Battery for the Assessment of Neuropsychological Status (RBANS)], social function [Personal and Social Performance Scale (PSP)] and serum prolactin (PRL) levels before and after treatment were compared between the two groups, and the adverse reactions were counted. Results: Comparing with the control group (75.00%), the total effective rate of treatment in the observation group (95.00%) was higher(P<0.05). After treatment, the mental symptom score of the observation group was lower, and the RBANS score and PSP score were significantly higher comparing with the control group(P<0.05). After treatment, the serum PRL of the two groups increased significantly(P<0.05), and the difference between the two groups shows no significant (P>0.05). Compared with the control group, there was no significant increase in the incidence of adverse reactions in the observation group (P>0.05). Conclusion: On the basis of sulpiride tablets, the combination of lurasidone tablets in patients with schizophrenia can improve the efficacy and cognitive function of patients, and has no significant effect on serum PRL, with good safety.

Objective： To investigate the efficacy of Feixiankang Granules combined with Tetrandrine Tablets in the treatment of pneumoconiosis and its effect on lung function and serum IL-8 and IFN-γ levels. Methods: From June 2021 to May 2023,72 patients with pneumoconiosis admitted to the outpatient or inpatient department of the occupational disease department of Hengyang Hospital of Traditional Chinese Medicine were selected as the research objects. According to the random number table method, the patients were randomly divided into control group(n=36) and observation group (n=36). Both groups were given conventional symptomatic treatment. The control group was treated with tetrandrine tablets on this basis, and the observation group was treated with Feixiankang granules on the basis of the control group. After 3 months of treatment, the clinical efficacy, TCM symptom scores, lung function [maximum ventilation volume (MVV) and forced expiratory volume in one second (FEV1)], serum IL-8, IFN-γ levels and adverse reactions during treatment were compared between the two groups. Results: The total effective rate of the observation group was higher than that of the control group (P<0.05). After treatment, the scores of main symptoms, secondary symptoms and total scores of TCM symptoms in the two groups decreased(P<0.05), and those in the observation group were lower(P<0.05). After treatment, MVV and FEV1 in both groups increased (P<0.05), and those in the observation group were higher(P<0.05). IL-8 decreased in both groups(P<0.05), and the observation group was lower(P<0.05). IFN-γ in both groups increased(P<0.05), and the observation group was higher(P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). Conclusion: Feixiankang granule combined with tetrandrine tablets can significantly improve the curative effect, improve lung function, reduce serum IL-8 level and increase IFN-γ level in patients with pneumoconiosis.

Objective: To explore the efficacy of ornidazole injection combined with latamoxef sodium in the treatment of pelvic inflammatory disease and its effect on serum inflammatory factors. Methods: A total of 97 patients with pelvic inflammatory disease admitted to Xiamen Fifth Hospital from June 2020 to May 2023 were randomly (random number table method) divided into control group (n=49) and observation group (n=48). The control group was treated with intravenous drip of latamoxef sodium, and the observation group was treated with intravenous drip of ornidazole + latamoxef sodium. The clinical efficacy, clinical symptom scores, pelvic effusion, inflammatory mass diameter and serum inflammatory factors [C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT)] levels before and after treatment were compared between the two groups. Results: The total effective rate of the observation group was higher than that of the control group (93.75% vs 77.55%, P<0.05). Compared with the control group, the clinical symptom score and serum CRP, IL-6, PCT levels were lower in the observation group after treatment, and the pelvic effusion volume and inflammatory mass diameter were smaller (P<0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (8.33% vs 6.12%, P>0.05). Conclusion: The combined use of ornidazole injection can significantly improve the efficacy of latamoxef sodium alone in the treatment of pelvic inflammatory disease, improve clinical symptoms and signs, and reduce the level of serum inflammatory factors.

Objective： To investigate the effects of sacubitril valsartan on ventricular remodeling, myocardial fibrosis and serum angiogenesis factors in patients with chronic ischemic heart failure (CIHF). Methods: A total of 200 patients with CIHF were randomly divided into observation group (100 cases) and control group (100 cases), which were treated with sacubitril valsartan and benazepril hydrochloride, respectively. The course of treatment in both groups was 6 months. Observe the curative effect of two groups. The cardiac function indexes [left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDD)], 6-minute walking distance (6MWT), Minnesota Living with Heart Failure Questionnaire (MLHFQ) score, myocardial fibrosis indexes [transforming growth factor-β1 (TGF-β1), soluble growth stimulating gene 2 protein (sST2)] and angiogenesis factors [vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), angiotensin-1 (Ang 1)] were statistically analyzed before and after treatment in the two groups. Results: Comparing with the control group (71.13%), the observation group's total effective rate (86.46%) was higher (P<0.05). After treatment, the levels of NT-proBNP, LVEDD and MLHFQ score in the observation group were significantly lower comparing with the control group (P<0.05), while LVEF and 6MWT were significantly higher (P<0.05). After treatment, the serum levels of TGF-β1, sST2 and TIMP-1 in the observation group were significantly lower comparing with the control group (P<0.05), and the levels of VEGF, IGF-1 and Ang 1 were significantly higher (P<0.05). Conclusion: For CIHF, sacubitril valsartan can effectively regulate myocardial fibrosis indexes and angiogenesis factors, reverse ventricular remodeling, improve cardiac function and quality of life of patients, and has a good effect.

Depression is a chronic syndrome with complex etiology. Nearly one-third of patients are not sensitive to current antidepressants. In order to ensure the quality of life of all patients with depression, it is essential to explore new antidepressant treatment methods. Neuroactive steroids are metabolites of steroid hormones play an antidepressant role through multiple pathways and multiple targets. In this paper, the literature on neuroactive steroids in the treatment of depression was summarized to provide scientific basis for neuroactive steroids in the treatment of depression, and to provide new ideas for the development of antidepressant drugs.

Mitochondrial dynamics is the dynamic balance process in which mitochondria continuously divide and fuse under physiological or stimulating conditions, thereby regulating the distribution, quantity, size and shape of mitochondria in the cell. In cells, the regulation of mitochondrial dynamics is closely related to the functions of mitochondria, which include providing energy, regulating calcium homeostasis, participating in programmed cell death, cell signal transduction, and cell metabolism. In cancer, mitochondrial dynamic regulation is closely related to the proliferation, migration, treatment and drug resistance of tumor cells. Therefore, mitochondrial dynamic regulation has become a research hotspot in the field of cancer, and has shown great potential in tumor therapy. In this review, we review the key features that mediate mitochondrial dynamic regulation, and discuss the mechanisms involved in tumorigenesis and development and its potential in tumor therapy.