Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells

Abstract

Abstract: Objective: To study the antiproliferation effects of petasin on human neuroblastoma SK-N-SH cells and the possible mechanisms involved. Methods: The inhibitory effects of petasin on proliferation of tumor cells was detected by SRB assay; the effects of petasin on cell cycle arrest of SK-N-SH were measured by flow cytometry; the effects of apoptosis were examined by Hochest 33258 staining and flow cytometry and the phosphorylation of ERK1/2 of SK-N-SH was determined by western blotting. Results: Compared to that of other cancer cell lines such as SGC7901, SPC-A-1, SMMC 7721, Ec9706, SK-N-BE (2) and U251, the proliferation of SK-N-SH cells were strongly inhibited by petasin. After treated with petasin, the cell cycle of SK-N-SH arrested in G0/G1 phase, and there was no apoptosis detected either by Hoechst 33258 staining or by flow cytometry analysis. Finally, the phosphorylation of ERK1/2 was decreased by pretreatment with petasin in a dose-dependent way. Conclusion: Petasin is a potential anti-tumor of natural compounds, and shows the strong antiproliferation effect on SK-N-SH cells without inducing apoptosis. The inhibition of ERK1/2 phosphorylation might be involved in the antiproliferation effects of petasin.

Key words: petasin, SK-N-SH, cell, proliferation, ERK1/2

CLC Number:

R961

Cheng Yue-fang, Wang Cai-fang, Li Wen-jie, Lin Qing, Zhang Qi. Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells[J]. Acta Neuropharmacologica, 2011, 1(3): 1-6.

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