Effects of estradiol on learning and memory in ovariectomized rats: relationship with the contents of amino acids in the cerebrum

Abstract

Abstract: Objective: To investigate the influence of estradiol on learning and memory and the content of amino acids in the cerebrum of ovariectomized rats. Methods: Adult female rats were treated daily with subcutaneous injection of estradiol (75, 300 μg•kg-1) between weeks 7 and 16 after ovariectomy (OVX) or sham operation. The learning and memory of the rats was tested by a Morris water maze task on week 15, after which they were sacrificed and the content of amino acids in the cerebrum measured with high performance liquid chromatography (HPLC). Results: The ability of learning and memory was significantly improved, and the content of glutamic acid and the ratio of glutamic acid to γ-aminobutyric acid （Glu/GABA） in prefrontal cortex and hippocampus of OVX group was significantly decreased as compared to those of sham group. In addition, the ability of learning and memory of estradiol（75 μg•kg-1）treated group was significantly improved and the content of Glu and Glu/GABA in prefrontal cortex and hippocampus were significantly increased as compared to those of OVX group. However, the Glu/GABA ratio remained unchanged in the hippocampus of estradiol (300 μg•kg-1) treated group as compared to that of OVX group; and the ability of learning and memory in these rats did not significantly improve. Conclusion: Estradiol (75 μg•kg-1) improves the ability of learning and memory in OVX rats, and the increased Glu/GABA ratio in hippocampus possibly contributes to such an effect.

Key words: estradiol, ovariectomy（OVX）, high performance liquid chromato- graphy, glutamic acid, &, gamma, -aminobutyric acid (GABA), glutamic acid/&, gamma, -aminobutyric acid (Glu/GABA)

CLC Number:

ZHANG Xia-wei, ZHANG Dan-shen, XUE Gui-ping, ZHAO Yi-jie, JIN Can. Effects of estradiol on learning and memory in ovariectomized rats: relationship with the contents of amino acids in the cerebrum[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(5): 8-13.

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