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26 October 2019, Volume 9 Issue 5 Previous Issue    Next Issue

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Effects of Suaibing Essential Oil on Anti-Hypoxia Ability of Mice WEI Zhen-zhen，FANG Xiao-yan，BAI Ming，MIAO Ming-san，FAN Shuai-ke 2019, 9 (5):  1-4.  DOI: 10.3969/j.issn.2095-1396.2019.05.001 Abstract ( 57 )   PDF (1053KB) ( 23 )   Objective：To investigate the effect of Suaibing essential oil on the antihypoxia ability of mice. Methods：Through the establishment of mice hypoxia model under normal pressure，hypoxia model under reduced pressure and mice hypoxia model induced by isoproterenol，Suaibing essential oil was applied to the head，face and chest of mice. After being applied with essential oil，the mice were placed in a closed environment. The survival time of mice was recorded，and distilled water was used as a blank control group to observe the effect of SuAibing essential oil on anti-hypoxia ability of mice. Results：The experimental results show that Suaibing essential oil can significantly prolong the survival time of normal pressure sealed hypoxia，hypobaric hypoxia，and isoproterenol hypoxic mice（P<0.01）. Conclusion：Suaibing essential oil can significantly improve the body’s hypoxia tolerance. References | Related Articles | Metrics

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Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF NI Shuang，MIAO Ze-yuan，WANG Jia-xin，et al 2019, 9 (5):  5-9.  DOI: 10.3969/j.issn.2095-1396.2019.05.002 Abstract ( 85 )   PDF (2596KB) ( 26 )   Objective：To investigate the effect of BDNF combined with DMSO in inducing BMSCs differentiation into neuron-like cells，and to detect the expression of miRNA-26a in the process of neuron differentiation. Methods：BMSCs were isolated from rat femoral bone marrow and preinduced by DMSO. BMSCs were then cultured in a neuronal medium containing BDNF and differentiated into neurons. Differentiated neuron-like cell-specific marker neuron-specific enolase（NSE）was identified using immunohistochemical. Real-time quantitative PCR was used to detect the expression of PTEN/mTOR and miRNA-26a before and after neurodifferentiation. Results：BDNF combined with DMSO had significant effect in inducing BMSCs differentiation into neuron-like cells，and the contents of miRNA-26a and PTEN/mTOR were significantly increased during the process of neurodifferentiation. Conclusion：Mixed inducers of DMSO and BDNF can induce BMSCs differentiation into neuron-like cells in rats，and miRNA-26a may regulate neuronal differentiation through PTEN/mTOR. References | Related Articles | Metrics

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Batch Determination of Amino Acid Neurotransmitters and Taurine in Mouse Cortex and Hippocampus by Amino Acid Analyzer WU Chun-yang，MA Jia-cheng，ZHANG Nan，et al 2019, 9 (5):  10-16.  DOI: 10.3969/j.issn.2095-1396.2019.05.003 Abstract ( 74 )   PDF (1390KB) ( 27 )   Objective：To establish a simple，accurate and reliable method for the batching detection of amino acid neurotransmitters and taurine in brain. Methods：The amino acids were detected by using A300 amino acid analyzer. The protein was precipitated by using 10% sulfosalicylic acid. Lithium salt reagent was used as mobile phase and ninhydrin was used as color reagent. The detection wavelength was 570 nm. The resolution，linear range，detection limit and quantitative limit，intraday precision and recovery，daytime precision of the method were determined. SPF-class KM mice at the same age were selected and divided into two groups：male and female. Three in each group were decapitated and executed，and each mouse was separated the cortex and hippocampus from the brain on ice. The free amino acid samples of mouse brain tissue were prepared by homogenization，centrifugation，protein precipitation，dilution and membrane transmembrane. The contents of aspartic acid（Asp），glutamic acid（Glu），glycine （Gly），gamma-aminobutyric acid（GABA）and Tau in cortex and hippocampus were determined by amino acid analyzer. Results：Five target amino acids in standard samples，cortical and hippocampal samples of male and female mice were well separated. Within the concentration range of 8~400 μmol·L-1 ，the instrument had a good linear relationship（R2 ≥0.999）. Detection limit was 1.29~1.86 μmol·L-1 . Quantitative limit was 4.29~6.20 μmol·L-1 . The intraday relative standard deviation（RSD）of standard samples were less than 2.23%，the intraday RSD of brain tissue samples were less than 2.76% and the recovery of the spiked samples were in a range of 91.52%~102.51%. 5 kinds of amino acid content in the sample were accurately obtained by plotting the peak area curve of the standard sample. No statistically significant difference was found between the male and female mice（P>0.05）. Conclusion：The method is simple，accurate， reliable and suitable for batching detection of free amino acid content in mouse cortex and hippocampus. References | Related Articles | Metrics

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Methods and Evaluation of Animal Models of Induced Pneumonia ZHANG Dan-shen，ZHANG Jian-mei 2019, 9 (5):  17-23.  DOI: 10.3969/j.issn.2095-1396.2019.05.004 Abstract ( 127 )   PDF (1098KB) ( 48 )   Pneumonia has a high incidence and mortality rate in infants，aging and immunocompromised populations. Establishing an effective and stable animal model is an important foundation for basic research and clinical research related to pneumonia. This article provides more options for basic and clinical research applications through detailing description and evaluation of animal models of induced pneumonia. References | Related Articles | Metrics

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Methods and Evaluation of Animal Models of Induced Pulmonary Hypertension ZHANG Dan-shen，LI Jia-ying 2019, 9 (5):  24-29.  DOI: 10.3969/j.issn.2095-1396.2019.05.005 Abstract ( 102 )   PDF (1087KB) ( 58 )   Pulmonary hypertension is a disease developed as a result of pulmonary vascular remodeling. Its pathogenesis is not yet clear. The animal model of anoxic pulmonary hypertension was prepared，and the standardized and suitable experimental animals were selected for experiment in this paper，the existing animal models of pulmonary hypertension were described and evaluated in detail. It provides a reference for clinical research. References | Related Articles | Metrics

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Methods and Evaluation of Animal Models of Induced Emphysema ZHANG Dan-shen，LI Lan 2019, 9 (5):  30-33.  DOI: 10.3969/j.issn.2095-1396.2019.05.006 Abstract ( 61 )   PDF (1081KB) ( 38 )   Emphysema is an incompletely reversible chronic respiratory disease characterized by progressive development of air?ow limitation. Establishing an ideal emphysema model can provide a theoretical basis for the prevention and treatment of emphysema. The detailed description and evaluation can provide more options for basic and clinical research applications. References | Related Articles | Metrics

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Methods and Evaluation of Animal Models of Induced Pulmonary Edema ZHANG Dan-shen，WANG Fei-fan 2019, 9 (5):  34-39.  DOI: 10.3969/j.issn.2095-1396.2019.05.007 Abstract ( 177 )   PDF (1105KB) ( 79 )   Pulmonary edema is a relatively rare disease. Mostly，for a certain reason，a large amount of tissue fluid cannot be absorbed by the pulmonary lymph and venous system in a short time，and pulmonary edema occurs. Pulmonary edema refers to the pathological state in which the fluid in the pulmonary capillaries infiltrates into the interstitial lung and alveoli，and the amount of extrapulmonary fluid increases. It is one of the clinical emergencies，and there are many hypotheses about its mechanism，and each has experimental basis，but both the process of the occurrence and development of pulmonary edema cannot be fully explained. Therefore，some animal models are often used to study the pathogenesis of the disease and related experimental research. This article is of great significance to the application of basic and clinical research through the detailed description and evaluation of the method of inducing pulmonary edema in animals. References | Related Articles | Metrics

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Toxoplasma Infection on Nerve Tissue Damage and Its Mechanism through the Blood-Brain Barrier YANG Jing，YUAN Wen-ying 2019, 9 (5):  40-43.  DOI: 10.3969/j.issn.2095-1396.2019.05.008 Abstract ( 171 )   PDF (1053KB) ( 50 )   Toxoplasma gondii is an obligate intracellular parasitic protozoa that invades almost all nucleated cells. The worm enters the body and spreads to the tissues and organs along with blood circulation，causing toxoplasmosis. Toxoplasma gondii has a tropism on nerve tissue，causing abnormalities in the central nervous system，often manifested as encephalitis， meningoencephalitis，epilepsy and mental disorders. The damage of Toxoplasma gondii to nerve tissue has been widely concerned. The blood-brain barrier is a place where the brain tissue communicates with the outside world. Its unique structure delays or blocks the process of Toxoplasma gondii invasion into the brain to a certain extent，but Toxoplasma gondii still invades the central nervous system through the blood-brain barrier，causing the tissue cell damage in host brain. This paper describes the damage of Toxoplasma gondii to the central nervous system and the mechanism through the blood-brain barrier. References | Related Articles | Metrics

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A New Idea to Treat Glioma with Mulberin ZHAO Peng-hui，PANG Ce，ZHEN Pan 2019, 9 (5):  44-49.  DOI: 10.3969/j.issn.2095-1396.2019.05.009 Abstract ( 54 )   PDF (1086KB) ( 25 )   Glioma is the primary brain tumor generated by cancerous brain and spinal cord glial cells. It’s the most common malignant tumor，comes from neuroepithelial cells and is mainly caused by EGF receptor mutation. Glioma cells also secrete VEGF factors，which can lead to more serious consequences. Currently，the treatments for the disease include surgery， radiotherapy，chemotherapy and other means，however，it can only relieve the symptoms，be completely cured hardly and often relapse. In recent years，the researches of drug therapy have not been stopped. Morus alba L is the root bark of Morus alba L， which is mainly used for treating lung heat，wheezing and phlegm，stopping lung by drinking water，and wheezing and acute wheezing after swelling. The Mulberin extracted from Morus alba L is the mainly active ingredient. Studies have shown that Mulberin has a certain effect on EGF in cancer cells，which in turn affects the expression of EGF receptor. Mulberin also has a certain effect on VEGF，so it can be used to treat Glioma by affecting EGF and VEGF. This article reviewed the development status of Glioma，the research of Mulberin and its effect on EGF and VEGF. In the meantime，a new idea of treating Glioma with Mulberin is proposed based on above of conclusions. References | Related Articles | Metrics

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Research Progress of Brain-Derived Estrogen in Alzheimer’s Disease YANG-Lin，AI-Jing 2019, 9 (5):  50-64.  DOI: 10.3969/j.issn.2095-1396.2019.05.010 Abstract ( 78 )   PDF (3634KB) ( 22 )   Epidemiological studies have shown that the risk of Alzheimer’s disease （AD）in postmenopausal women is much higher than that in men. Numerous clinical trials have indicated that decreased level of brain-derived estrogen in women is related to the risk of AD. This review focuses on the synthesis，metabolism and action mechanisms of estrogen（especially brain-derived estrogen），the role of brain estrogen receptors（ERs），brain-derived estrogen in the regulation of cognitive function and brain-derived estrogen dysfunction in the progression of AD. References | Related Articles | Metrics