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26 October 2015, Volume 5 Issue 5 Previous Issue    Next Issue

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Effect of Resveratrol on Longevity of Caenorhabditis Elegans and Its Potential Mechanism WANG Ting-ting, ZHANG Yu-yao, WANG Ji-xi, LIU Yang, CHEN Yong-chun, JIANG Guo-hua 2015, 5 (5):  1-6.  Abstract ( 1877 )   PDF (1165KB) ( 1404 )   Objective：To investigate the effect of resveratrol on longevity of Caenorhabditis elegans （C.elegans） and its potential mechanism. Methods：Three types of C.elegans，wild type （N2），daf-2 mutant（ e1370） and daf-16 mutant（ mu86） were cultured with different concentrations of resveratrol （0、25、50、100 μmol·L-1）. The survival rates were measured and the effect of resveratrol on life span was recorded. Results：Compared with the DMSO group，significantly extension of the normal life span of three types of C.elegans（ N2，daf-2 and daf-16 mutants） was observed containing 25、50、100 μmol·L-1 resveratrol. Conclusion：Resveratrol can extend the life span of C.elegans，possible not associated with DAF-2/insulin-like signaling pathway. References | Related Articles | Metrics

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Effect of Kangnaoye on Nerve Regeneration Related Factor in Rats of Cerebral Ischemia-Reperfusion Injury SUN Yan, SONG Ai-xia, XUE Qian, ZOU Yu-an 2015, 5 (5):  7-14.  Abstract ( 1932 )   PDF (8020KB) ( 902 )   Objective：To observe the effect of Kangnaoye on nerve regeneration related factor in rats of cerebral ischemia-reperfusion injury. Methods：150 healthy adult male Sprague Dawley rats were randomly divided into 5 groups；Kangnaoye high，middle and low dose group （24、12、6 g·kg-1·d-1），sham operated group and model group. Longa method was used to establish the middle cerebral artery occlusion（MACO） model. TTC staining method was used to observe infarction volume change in rats after 24 h. Collecting specimens in 1 d、3 d、7 d and 14 d，immunohistochemical method was used to observe the change of the growth associated protein-43 （GAP-43） after cerebral ischemic and the expression of neurite outgrowth inhibitor-A （NOGO-A） protein .The nervous function deficit scores were evaluated at 2 h、1 d、3 d、7 d、14 d after reperfusion. Results：Compared with model group，the neurological functions were improved significantly in kangnaoye groups（P<0.05）. Kangnaoye high，middle and low dose groups were significantly decreased than model group in cerebral infarction volume ratio （P<0.01）. The expression levels of GAP-43 were also significantly higher in kangnaoye high and middle dosages groups than model group，at the same time the expression of NOGO-A were lower，which has significant differences （P<0.05 or P<0.01）. Conclusion：Kangnaoye can promote neurotization and improve the motor skill recovery after cerebral infarction. References | Related Articles | Metrics

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Effects of Xingnaojing Injection on hs-CRP、IL-6 and TC in Patients with Acute Ischemic Stroke LIU Song 2015, 5 (5):  15-19.  Abstract ( 1680 )   PDF (1082KB) ( 1263 )   Objective：To observe the effects of Xingnaojing injection on acute ischemic stroke patients with high-sensitivity CRP（hs-CRP），interleukin-6（IL-6） and total cholesterd（TC） level，and to explore its possible mechanism. Methods：100 patients with acute ischemic stroke were treated in department of neurology of hospital of Nanyang Nan Shi from December 2013 to October 2015. They were randomly divided into observation group and control group，50 cases in each group. Control group patients were given regular treatment，while the patients in observation group were given Xingnaojing injection based on regular treatment，once a day for 2 weeks（ one course）. Venous blood of all patients was drawn to detect the levels of hs-CRP，IL-6 and TC before treatment and after treatment for first，3，6，14，10 days. Results：The levels of hs-CRP，IL-6 and TC in all patients showed a slow downward trend，while compared with the control group，the observation group was decreased more significantly（ P<0.05）. The neurological rehabilitation of observation group were significantly higher than control group（P<0.05）. Conclusion： Xingnaojing injection can significantly decrease the level of hs-CRP，IL-6 and TC in serum of patients with acute ischemis level，which has a neuroprotective function. References | Related Articles | Metrics

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Study on the Distribution and Drug Releasing in Digestive Tract of Colon-Targeted Pellets of Rhubarb in Rats ZHANG Dan-shen, MEI Yan-fei, SONG Xiao-min, ZHUANG Zhong-bao, XUE Gui-ping 2015, 5 (5):  20-27.  Abstract ( 1864 )   PDF (1232KB) ( 1252 )   Objective：To investigate the conveying and drug release in vivo of colon target pellets of Rhubarb in rats. Methods：The rhubarb colon-targeted pellets were administered by homemade rat mouth gag. The rats were respectively killed at different time points after administering，and the gastrointestinal tracts were dissected. The appearance of pellets was observed and the sum of pellets were counted. The pellets distribution rates in the digestive tract in rats were calculated at different time point after administering. After the residue level of aloeemodin， emodin and chrysophanol in pellets were respectively determined by HPLC，also the emodin in rats，gastrointestinal tissue and content. The accumulative releasing was calculated and the drug concentration-time curve in the gastrointestinal tract was drawn，then the drug release property was evaluated. Results：The pellets were transferred to colon completely and obvious late release effect. The colon distribution rate could reach the highest after 10~12 h，52.7%. In 8~16 h， drugs had a high concentration in the cecum and colon. Final release dosage percentage was 88.99%. Conclusion：Rhubarb colon-targeted pellets possessed the characteristic of drug release in colon. References | Related Articles | Metrics

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Research Progress on the Effect of Neural Stem Cells in the Occurrence and Development of Glioma LUO Lin-ming, JI Yi-na, CHEN Nai-hong 2015, 5 (5):  28-33.  Abstract ( 1740 )   PDF (1077KB) ( 1529 )   Neural stem cells have the properties of self-renewal，continuous proliferation and multidifferentiation，which play an important role in the occurrence and development of glioma. The occurrence and development of glioma is a complex multi-step process，involving the changes of a number of related genes，transcription factors，signaling pathways and their associated proteins，as well as some growth factors and enzymes in expression and regulation. References | Related Articles | Metrics

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Eff ects of Isofl urane On Neurocognitive Function and Its Possible Mechanism WU Bei, WANG Xin-sheng, YANG Yue-ping,TENG Jin-liang 2015, 5 (5):  34-39.  Abstract ( 1737 )   PDF (1097KB) ( 1407 )   A lot of experimental evidences showed that isoflurane can cause the change of learning and cognitive ability，resulting from the inhibition of N-methyl-D-aspartic acid（ NMDA） receptor，depolarization induced by activation of gamma aminobutyric acid（ GABA） receptor，Ca2+ influx and promote activation of caspase-3 which leads to neuronal toxicity. Volatile anesthetics could interfere with physiologic patterns of synaptogenesis and impair density of endritic spines of neurons in the developing cerebral cortex，thus causing changes in behavior. Isoflurane excessively activates calmodulin，inhibits hippocampal synaptic long term potential（ LTP） and curbs short-term memory into long-term memory conversion. Isoflurane induces PERK phosphorylation and increases the expression of CHOP by a series of molecular mechanisms. CHOP up-regulates pro-apoptotic proteins and downregulates anti-apoptotic proteins to promote neuronal apoptosis. Tau protein is highly phosphorylated in the form of nerve fibers and the formation of nerve fibers is considered as a common pathological pathway in the pathogenesis of Alzheimer’s disease. In this review，the mechanism of the general anesthetics inducing consciousness loss and its effect of central nervous system were summarized. References | Related Articles | Metrics

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Tissue Localization and Physiological Functions of Urea Transporters JIANG Tao，YANG Bao-xue 2015, 5 (5):  40-48.  Abstract ( 1801 )   PDF (1158KB) ( 1177 )   Urea transporters（ UT） are a class of transmembrane proteins that are selectively permeable to urea. There are two UT subfamilies，six members（ UT-A1-A6） in UT-A subfamily that are mainly expressed in kidney. There is only one member（ UT-B） in UT-B subfamily that is widely distributed in multiple tissues，including the red blood cell，kidney，brain，bladder，testis，etc. Urea transporters play an important role in the urine concentration mechanism，and are also involved in many other physiological functions. In this review article，we summarize the expression localization and physiological functions of urea transporters. References | Related Articles | Metrics

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Progress of Voltage Gated Sodium Channel Nav1.7 and its Specific Blockers in Nneuropathic pain WANG Chuan，SHAN Bin，WANG Qiong，ZHANG Hai-lin 2015, 5 (5):  49-56.  Abstract ( 1627 )   PDF (1169KB) ( 1165 )   Voltage gated sodium channels (Nav) play important roles in generation and conduction of pain. In pain conduction pathway, Nav1.7 is selectively expressed in small-diameter peripheral somatic sensory neurons and sympathetic ganglion neurons. Nav1.7 modulates neuronal excitability through amplifing subthreshold of depolarizations and setting up the thresholds of Nav1.8 and Nav1.9. This review will focus on the progress of neuropathic pain caused by mutations in Nav1.7. Nav1.7 can be used as an effective target for the treatment of pain. Highly selective Nav1.7 blockers will be of great clinical value in the treatment of pain. References | Related Articles | Metrics

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Research Progress on the Protective Eff ect of Cerebral Ischemia Preconditioning on Cerebral Ischemia Injury ZHANG Jing, ZOU Yu-an, Dong Xiao-hua 2015, 5 (5):  57-64.  Abstract ( 1702 )   PDF (1123KB) ( 1231 )   Cerebral ischemic preconditioning（ CIP） is a method of endogenous mechanical stimulation of cerebral tissue，such as inducing the reperfusion stimulation by once，or repeatedly transient cerebral ischemia and non-fatal cerebral ischemia. In this way the cerebral tissue will produce the endogenesis defense mechanism.The cerebral tissue will generate significant tolerance of lethal ischemia，and weaken or block the cascade reaction induced by cerebral ischemia or cerebral anoxia. And this phenomenon is also called cerebral ischemia tolerance （CIT）. Cerebral ischemia-reperfusion injury（CIRI） is a physiological process of clinical pathology that is involoved in multipal mechanism. It could further aggravate the extent of cerebral tissue damage and dysfunction，and even irreversible damage occurs after restoration of blood flow and reperfusion. All in all，CIP plays a protective role to CIRI. This review will summarize the related neural protection mechanism and important pathways about CIP for CIRI protection. References | Related Articles | Metrics