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Exploring the Effect and Mechanism of Pterostilbene on Alzheimer’s Disease Based on Network Pharmacology ACTA NEUROPHARMACOLOGICA    2023, 13 (5): 1-.   DOI: 10.3969/j.issn.2095-1396.2023.05.001 Abstract （6）      PDF（pc） （4504KB）（8）       Knowledge map    Save Objective: Based on network pharmacology, the study aims to explore the potential target and signaling pathway of pterostilbene against Alzheimer's disease(AD). Methods: Swiss Target Prediction database was used to screen the potential targets of pterostilbene; Gene Cards database was used to retrieve the relevant targets of Alzheimer’s disease; Venny2.1 website was employed to obtain the intersection of drug-disease target genes; STRING database was used to construct the protein-protein interaction (PPI) network of common genes; Cytoscape 3.7.2 software was utilized for visualization and analysis of the pterostilbene-target network; DAVID database was employed for gene ontology (GO) enrichment analysis and kyoto gene and genome encyclopedia (KEGG) signaling pathway enrichment analysis of common genes; Online tools were used to draw GO and KEGG pathway enrichment analysis diagrams. Results: By database screening, 100 potential targets of pterostilbene and 14472 Alzheimer’s disease-related targets were obtained, among which 88 were identified as potential targets of pterostilbene in Alzheimer’s disease. Base on the analysis of Betweenness, ESR1, EGFR, MAPT, PTGS2 and BCL2 were core targets. Enrichment analysis showed that endocrine resistance, neurotrophin signaling pathway and prolactin signaling pathway were highly involved. Conclusion: Pterostilbene may regulate endocrine resistance and neurotrophic signaling pathways through core targets such as ESR1, EGFR, MAPT, PTGS2 and BCL2 to exert its protective effect against Alzheimer’s disease. Related Articles | Metrics | Comments（0）

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Cyberpharmacology-Based Analysis of the Molecular Mechanism of Dokuto Parasite Soup in the Treatment of Intervertebral Disc Degeneration TAO Yu-jing, WANG Xiang-fu, XIE Yu-tao, et al ACTA NEUROPHARMACOLOGICA    2023, 13 (4): 1-.   DOI: 10.3969/j.issn.2095-1396.2023.04.001 Abstract （3）      PDF（pc） （8885KB）（4）       Knowledge map    Save Objective: To analyze the possible mechanism of action of Duluxiaosheng Tang in the treatment of intervertebral disc degeneration (IDD) using network pharmacology. Methods: The bioactive components of Duluxiaosheng Tang were searched in the TCMSP platform and screened according to the research needs, while the targets of intervertebral disc degeneration were searched in the GeneCards and OMIM databases; the "drug component-target-disease" complex network was constructed using Cytoscape software. A complex network of "drug component-target-disease" was constructed using Cytoscape software, and a Wayne diagram was constructed to obtain the targets for IDD treatment with Doklam parasitic soup. Results: A total of 240 active ingredients, 257 potential targets, and 2713 targets of intervertebral disc degeneration disease were explored in Doklam parasitic soup, and 75 common targets were obtained. 20 entries were obtained from GO enrichment analysis and 20 entries from KEGG pathway enrichment analysis. Conclusion: Doklam parasitic soup may regulate AGE-RAGE signaling pathway in diabetic complications, Lipidandat herosclerosis and IL- by modulating MAPK3, FOS, RELA, TP53, IL-6, MAPK1, TNF and other targets. 17 signaling pathway to inhibit inflammatory response and regulate apoptosis to treat intervertebral disc degeneration. Related Articles | Metrics | Comments（0）

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Study on the Analgesic Effect of Resveratrol in the Context of Pharmacognosy CHEN Yan-ming, CHEN Jin-song ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 6-.   DOI: 10.3969/j.issn.2095-1396.2023.06.002 Abstract （3）      PDF（pc） （1335KB）（3）       Knowledge map    Save Objective: The food and medicine industry is an integral part of the national strategy to develop a “healthy China”. Resveratrol is a naturally occurring compound that has been shown to be biologically active in various diseases such as inflammation, heart disease and cancer. The aim of this study was to investigate the analgesic effects of resveratrol in acute and chronic pain in mice. Methods: In this study, the analgesic effects of resveratrol were evaluated by constructing mouse models of acute pain (acetic acid-induced torsion, formalin-induced persistent pain) and chronic pain (paclitaxel-induced neuropathic pain). Results: The behavioural results showed that resveratrol was effective in reducing acute and chronic pain behaviour in mice and exhibited beneficial effects. Conclusion: Therefore, resveratrol may be a potential drug for the treatment of acute and chronic pain in mice. This study provides a certain theoretical basis for subsequent related studies and offers new ideas and methods for safe and effective clinical analgesia. Related Articles | Metrics | Comments（0）

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Research Progress of Core Markers and Other Markers of Alzheimer's Disease YIN Hong-yan, YOU Si-han, GUO Chun-yan ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 45-.   DOI: 10.3969/j.issn.2095-1396.2023.06.009 Abstract （5）      PDF（pc） （976KB）（3）       Knowledge map    Save Alzheimer's disease （AD） is a neurodegenerative disease that cannot be cured until now. The main existing diagnostic methods are the detection of biomarkers in cerebrospinal fluid and positron emission tomography （PET）， but their application in clinical practice is hampered due to the invasiveness and high cost of their testing. Biomarkers in blood samples can make early detection and diagnosis of ad universal because of their easy availability and low price. This article summarizes several common biomarkers of AD and their changes in AD patients， and prospects the development of ad biomarkers in the future. Related Articles | Metrics | Comments（0）

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Research Status on the Neuroprotective Effect of Probucol ZHU Jing-yi , FAN Jian-chun, YU Ai-mei ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 50-.   DOI: 10.3969/j.issn.2095-1396.2023.06.010 Abstract （4）      PDF（pc） （926KB）（3）       Knowledge map    Save Probucol has two easily oxidized phenolic hydroxyl groups， which can combine with oxygen free radicals to form a stable phenolic oxygen group. It has powerful lipid-lowering， anti-inflammatory and antioxidant functions and plays an important role in the treatment process. This article reviews the protective effects and detailed protective mechanisms of probucol in the central nervous system and peripheral nervous system based on domestic and foreign literature in recent years. Related Articles | Metrics | Comments（0）

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Mechanism of Action of Vaccine Adjuvants and Research Progress ZHANG Xin-yan, SHI Xiao-zi, ZHAO Yong-ming ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 55-.   DOI: 10.3969/j.issn.2095-1396.2023.06.011 Abstract （4）      PDF（pc） （961KB）（3）       Knowledge map    Save Vaccination is currently the most economically effective method for preventing infectious diseases. Adjuvants are substances added to vaccines to enhance antigens immunogenicity. The appropriate addition of adjuvants can better assist vaccines in stimulating the body， achieving efficient and long-lasting immune responses， reducing the production costs of vaccines， and minimizing the dosage of drugs， thus decreasing the toxic side effects of drugs entering the human body. This article provides a comprehensive review of commonly used adjuvants and their immunogenic properties. It aims to serve as a reference for the design and selection of safe and effective vaccine adjuvants. Related Articles | Metrics | Comments（0）

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To study the Mechanism of Astragalus Bupleurum on Hyperthyroidism Based on Network Pharmacology CHEN Fan, REN Jun-xu, ZHANG Jing ACTA NEUROPHARMACOLOGICA    2023, 13 (5): 6-.   DOI: 10.3969/j.issn.2095-1396.2023.05.002 Abstract （3）      PDF（pc） （3318KB）（2）       Knowledge map    Save Objective: The mechanism of Astragalus-Bupleurum treatment for hyperthyroidism was investigated by network pharmacology and molecular docking methods. Methods: TCMSP database was used to screen out the active constituents of Astragalus bupleurum and their corresponding targets with bioavailability (OB)≥30% and drug index (DL)≥0.18. Both GeneCards, DisGeNET, TTD and Drugbank databases were used to search the relevant targets with the key words Hyperthyroidism; To obtain the intersection targets between TCM and diseases, the key targets were selected using the "Degree", "Betweenness" and "Closeness" in Cytoscape software, and the key target information was obtained on the DAVID platform to construct GO functional enrichment and KEGG pathway enrichment analysis. Autodock Tools 1.5.7 software was used to conduct molecular docking of core targets and their corresponding active components. Results: The results showed that 20 active ingredients (224 targets) of Astragalus and 17 active ingredients (193 targets) of Bupleurum were obtained. There were 1358 targets associated with hyperthyroidism. Intersection mapping obtained 76 intersection targets. Eighteen core targets were selected by the plug-in for GO functional enrichment analysis and 306 items were obtained, including 244 biological process items, 20 cell localization items and 42 molecular function items. Ninety-six signaling pathways were annotated by KEGG pathway, such as HIF-1 signaling pathway, IL-17 signaling pathway, TNF signaling pathway, etc. Molecular docking results showed that the key targets of AKT1, IL-6, TNF and IL-1β had good affinity with the corresponding active ingredients. Conclusion: Radix Astragalus Bupleurum is effective in treating hyperthyroidism through multi-component, multi-target and multi-pathway synergies. This study provides sufficient theoretical basis for subsequent research. Related Articles | Metrics | Comments（0）

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Excavation and Analysis of Compatibility Law and Application Characteristics of Prescriptions Containing Sanguisorbae Radix ZHANG Feng , ZHONG Juan, PAN Wen-bo, PEI Zhi-fei ACTA NEUROPHARMACOLOGICA    2023, 13 (5): 14-.   DOI: 10.3969/j.issn.2095-1396.2023.05.003 Abstract （2）      PDF（pc） （2898KB）（2）       Knowledge map    Save Objective: Based on data mining, to explore the compatibility law and application characteristics of prescriptions containing Sanguisorbae Radix. Methods: Taking "Sanguisorbae Radix" or "Sanguisorbae Radix root" as keywords, collecting prescriptions on Yaozhi.com and related classic books; Microsoft Office Excel 2017, VOSviewer1.6.15, IBM SPSS Modeler 18.0, IBM SPSS Statistics 25.0 and other software are utilized to perform frequency statistics, overall drug usage analysis,nature and flavor analysis, association rule analysis, cluster analysis, etc. Results: 445 prescriptions are included in this study, which involves a total of 329 traditional Chinese medicine. Among them, traditional Chinese medicine such as Angelicae Sinensis Radix; Glycyrrhiza uralensis Fisch; Astragali Radix; Paeoniae Radix Alba; Coptidis Rhizoma are often in combination with Sanguisorbae Radix. The medicinal properties of traditional Chinese medicine combined with Sanguisorbae Radix are mainly cold and warm; while the medicinal tastes are more bitter, pungent and sweet; in addition, the meridian generally belongs to liver, lung and spleen. While Sanguisorbae Radix is often compatible with traditional Chinese medicine of tonic, hemostasis, and heat-clearing. It is mostly applied to treat dysentery, hemorrhoids, metrorrhagia disorder. In the treatment of dysentery, traditional Chinese medicine such as Angelicae Sinensis Radix; Coptidis Rhizoma; Scutellariabaicalensis Georgi; Glycyrrhiza uralensis Fisch; Saussurea lappa are often compatible with Sanguisorbae Radix. While traditional Chinese medicine such as Sanguisorbae Radix, Angelicae Sinensis Radix; Sophorae Flos; Aurantii Fructus; Scutellariabaicalensis Georgi are the core compatibility in the treatment of hemorrhoids. The results of cluster analysis show that high-frequency traditional Chinese medicine in the treatment of the two diseases can be grouped into 9 groups and 6 groups respectively. Conclusion: Sanguisorbae Radix is often in combination with traditional Chinese medicine of invigorating deficiency, hemostasis, and clearing away heat, which regularly have the effects of clearing away heat and cooling blood, purging fire and detoxification, astringing sores and promoting muscle growth. It is mostly applied to treat dysentery, hemorrhoids, and metrorrhagia disorder in clinical application. Related Articles | Metrics | Comments（0）

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Thin Layer Identification and Determination of Paeonol Content in Moutan Cortex from Different Origins YIN Hong-yan, YOU Si-han, ZHAO Yong-ming, YAN Fan-li, CHEN Ming-xia, JIANG Hai-yan, GUO Chun-yan ACTA NEUROPHARMACOLOGICA    2023, 13 (5): 24-.   DOI: 10.3969/j.issn.2095-1396.2023.05.004 Abstract （1）      PDF（pc） （1159KB）（2）       Knowledge map    Save Objective: High performance liquid chromatography (HPLC) was used to determine the content of paeonol in Moutan Cortex of different origins, and thin-layer chromatography (TLC) was used to identify the herbs of Moutan Cortex of different origins. Methods: The separation was performed on a G-type thin-layer chromatography silica gel plate with cyclohexane-ethyl acetate-glacial acetic acid (4:1:0.1) as the unfolding agent, and the color was developed by spraying with 2% vanillic acid ethanol sulfate solution; the separation was carried out on a Chevonne C18 column (4.6 mm × 250 mm，5μm) with the elution of methanol-water (45:55 V/V) as the mobile phase, at the flow rate of 1.0 mL·min-1 and the detection wavelength of 274 nm. The mobile phase was methanol-water (45:55 V/V) at a flow rate of 1.0 mL·min-1, the detection wavelength was 274 nm, the column temperature was 30 ℃, and the injection volume was 10 μL. Results: The results showed that the thin-layer identification spots of 21 batches of peony dandelion herbs were clear, and the contents of dandelion were in the range of 2.7%～2.9%, which were higher than the pharmacopoeia standard, of which dandelion in Shandong province was the highest. Conclusion: The quality of peony bark grown in Shandong, Henan and Anhui is good, with good character, small differences between different batches, and uniform and stable quality. Related Articles | Metrics | Comments（0）

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Efficacy of Citicoline Combined with Alteplase in the Treatment of Acute Cerebral Infarction and Its Effect on Cerebral Blood Flow Velocity and Inflammatory Factors XU Jing, HU Wen-yi , LI Huang ACTA NEUROPHARMACOLOGICA    2023, 13 (5): 29-.   DOI: 10.3969/j.issn.2095-1396.2023.05.005 Abstract （1）      PDF（pc） （1027KB）（2）       Knowledge map    Save Objective: To investigate the efficacy of citicoline combined with alteplase in the treatment of acute cerebral infarction (ACI) and its effect on cerebral blood flow velocity and inflammatory factors. Methods: Sixty patients with ACI were divided into control group and experimental group by random number table method, with 30 cases in each group. Both groups were given conventional treatment such as anti-platelet aggregation and anticoagulation. In addition, the control group was treated with alteplase thrombolytic therapy, and the observation group was treated with citicoline combined with alteplase. Both groups were treated for 14 days. The neurological function (National Institutes of Health Stroke Scale (NIHSS)), daily living ability (Barthel Index (BI)), cerebral blood flow velocity and serum inflammatory factors (C-reactive protein (CRP), tumor necrosis factor-α (TNF-α)) of the two groups before and after treatment were statistically analyzed, and the efficacy and adverse reactions of the two groups were compared. Results: Compared with the control group (73.33%), the total effective rate of the observation group (96.67%) was higher (P<0.05). After treatment, compared with the control group, the NIHSS score of the observation group was lower(P<0.05), and the BI score was higher (P< 0.05). After treatment, the cerebral blood flow velocity in the observation group was increased(P<0.05), and the levels of serum CRP and TNF-α were decreased (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: Citicoline combined with alteplase is effective in the treatment of ACI, which can better reduce the neurological deficit of patients. The mechanism may be related to the improvement of cerebral blood flow velocity and the reduction of inflammation level. Related Articles | Metrics | Comments（0）

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Analysis of the Effect of Yigui Yufeng Decoction Combined with Acupuncture on the Curative Effect and Inflammatory Factor Level of Ischemic Strok ZHAO Han-qing, FENG Peng-chao, ZHOU Li-fei, LI Li ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 12-.   DOI: 10.3969/j.issn.2095-1396.2023.06.003 Abstract （4）      PDF（pc） （1001KB）（2）       Knowledge map    Save Objective： To investigate the clinical efficacy of Yigui Yufeng Decoction in patients with ischemic stroke and its effect on the level of inflammatory factors. Methods： 82 patients with ischemic stroke were randomly divided into treatment group and control group， 41 cases in each group. The control group was treated with conventional western medicine， and the treatment group was treated with Yigui Yufeng Decoction and acupuncture on the basis of the control group. The treatment time of the two groups was 14 days. The effective rate of treatment and the levels of serum high-sensitivity C-reactive protein （hs-CRP）， blood homocysteine （Hcy） and D-dimer before and after treatment were compared between the two groups. The activities of daily living scale （ADL score） and NIHSS scale score were compared between the two groups before and after intervention. Results： After 14 days of treatment， the effective rate of the treatment group was （ 92.68 %）， and that of the control group was （ 73.17 %）. The difference was statistically significant（ P <0.05）. After treatment， the levels of the above factors in the treatment group were lower than those in the control group （P <0.05）. After treatment， the ADL score of the treatment group was higher than that of the control group， and the NIHSS score was lower than that of the control group （P <0.05）. Conclusion： Yigui Yufeng Decoction combined with acupuncture has a good therapeutic effect on patients with ischemic stroke. It can significantly improve the clinical symptoms of patients， reduce the level of inflammatory factors in the body， and improve the quality of life and neurological function of patients. Related Articles | Metrics | Comments（0）

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Regulation Mechanism of Xijiao Dihuang Decoction on Coagulation Function in Septic Rats LIANG Zhi-qi, SHAO Dan, CHEN Hong-jie, LI An-qi, WU Hui ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 16-.   DOI: 10.3969/j.issn.2095-1396.2023.06.004 Abstract （4）      PDF（pc） （966KB）（2）       Knowledge map    Save Objective：To investigate the regulatory mechanism of Xijiao Dihuang decoction on coagulation function in septic rats. Methods： Ninety rats were randomly divided into treatment group， model group and sham operation group， with 30 rats in each group. The sepsis model was established by cecal ligation and puncture in the treatment group and the model group， and the cecum was gently turned without ligation and puncture in the sham-operation group. After successful modeling， the treatment group was given Xijiao Dihuang decoction by gavage， and the model group and sham operation group were given normal saline by gavage for 3 consecutive days. Plasma tissue factor（ TF）， antithrombin-III（ AT-Ⅲ）， activated protein C（ APC）， tissue factor pathway inhibitor （TFPI） and plasminogen activator inhibitor-1 （PAI-1） levels were detected by ELISA AT 24， 48 and 72 hours after gavage in the treatment group， model group and sham operation group. Results： There was no significant difference in TF， AT-Ⅲ， APC， TFPI and PAI-1 between the sham operation group and the control group AT 24， 48 and 72 h （P>0.05）. Compared with the sham operation group， TF， TFPI and PAI-1 in the model group increased and AT-Ⅲ and APC decreased AT 24， 48 and 72 h， and the differences were statistically significant （P<0.05）. In the model group， TF， AT-Ⅲ， APC， TFPI and PAI-1 showed progressive changes with time AT 24， 48 and 72 h， and the differences were statistically significant （P<0.05）. Compared with the model group， TF and PAI-1 in the treatment group decreased， while AT-Ⅲ， APC and TFPI increased AT 24， 48 and 72 h， and the differences were statistically significant （P<0.05）. The levels of TF， AT-Ⅲ， APC， TFPI and PAI-1 in the treatment group AT 24， 48 and 72 h showed progressive changes with time， and the differences were statistically significant （P<0.05）. Conclusion： The mechanism by which Xijiao Dihuang Decoction regulates coagulation function is related to the decrease of TF and PAI-1 and the increase of AT-Ⅲ， APC， and TFPI in septic rats， but further exploration is needed. Related Articles | Metrics | Comments（0）

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Efficacy and Safety of Tirofiban Combined with Thromboaspiration in Percutaneous Coronary Intervention Surgery： A Meta-Analysis YANG Bo, ZHAO Xiao-min, LU Meng, ZHANG Ze-peng, WU Chang-sha, LI Fang-jiang ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 21-.   DOI: 10.3969/j.issn.2095-1396.2023.06.005 Abstract （4）      PDF（pc） （1814KB）（2）       Knowledge map    Save Objective：To analyze the efficacy and safety of thrombus aspiration combined with intracoronary injection of tirofiban during percutaneous coronary intervention （PCI）. Methods： PubMed， Embase， Cochrane Library， CNKI， Wanfang Database， Weipu Database （VIP）， and China Biomedical Literature Database were searched in computer from the establishment of the library to the publication of the literature in November 2022. Quality was assessed by two investigators and statistically analyzed using Revman 5.3 software. Results： A total of 7 randomized controlled trials with a total of 1 152 patients were included， including 585 in the combination therapy group and 567 in the PCI alone group. Among them， 708 were males and 444 were females. According to the results of meta-analysis， the ST segment drop in patients treated with acute myocardial infarction （AMI） in the combination group was greater than 50% （RR=1.23， 95%CI 1.14~1.33， P<0.01）， postoperative TIMI grade 3 blood flow （RR=1.20， 95%CI 1.14~1.27，P<0.01）， left ventricular ejection fraction（ LVEF）（ MD=3.32， 95%CI 1.51~5.13，P<0.01）， left ventricular end diastolic diameter（ LVEDD）（ MD=-2.22， 95%CI -4.17~-0.28，P<0.05）， creatine kinase isoenzyme（ CKMB） peak （MD=-111.85， 95%CI -116.43~-107.27， P<0.01） were better than PCI alone， and the incidence of major adverse cardiovascular events （MACE） in the combination therapy group （RR=0.54， 95%CI 0.38~0.75， P<0.01） was lower than PCI alone. Conclusion： The efficacy and safety of the combination therapy group were better than those in the PCI alone group. Related Articles | Metrics | Comments（0）

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Study on the Effect and Neurohumoral Effect of Minimally Invasive Gastritis Resection Combined with Potassium Sodium Hydrogen Citrate in Patients with Giant Gastritis YANG Fu-song, YANG Yang, WANG Zhong-bo, ZENG Ying-Jie, GUO Hang ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 31-.   DOI: 10.3969/j.issn.2095-1396.2023.06.006 Abstract （2）      PDF（pc） （964KB）（2）       Knowledge map    Save Objective：To study the effect of minimally invasive gastritis resection combined with potassium sodium hydrogen citrate in patients with giant gastritis and its influence on neurofluid. Methods： 213 patients with giant gouty stones admitted to the First People's Hospital of Anning City from November 2020 to April 2022 were selected as the study objects. They were randomly divided into study group （n=107） and control group （n=106） by computer numbered odd-even number method. The control group was treated with traditional surgery combined with potassium sodium hydrogen citrate. In the study group，minimally invasive gastritis resection combined with potassium sodium hydrogen citrate was selected. The efficacy， pain degree， joint function， renal function index and neurohumoral factor level were compared between the two groups. Results： The total effective rate of the study group was higher than that of the control group（ 97.19% vs 86.79%）（ P<0.05）. The visual analogue scale（ VAS） scores of both groups were lower than those before surgery， but the Hospital for Special Surgery （HSS） scores were higher than those before surgery. The VAS score of the study group was lower than that of the control group， and the HSS score was higher than that of the control group （all P<0.05）. The levels of renal function indexes in both groups were lower after operation， and the study group was lower than the control group （all P<0.05）. The levels of neurohumoral factors in the two groups were higher than those before surgery， but those in the study group were lower than those in the control group（ all P<0.05）. Conclusion： The effect of minimally invasive gastritis resection combined with potassium sodium hydrogen citrate in the treatment of giant gastritis is better， which can effectively improve the pain degree， joint function， kidney function and neurohumoral factor level of patients. Related Articles | Metrics | Comments（0）

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Extraction and Content Determination of Total Volatile Oil in Perilla Leaf Grown in Zhangjiakou LUO Fei, WANG Ying, FENG Ting-ting, HE Wei ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 36-.   DOI: 10.3969/j.issn.2095-1396.2023.06.007 Abstract （4）      PDF（pc） （1149KB）（2）       Knowledge map    Save Objective：The contents of total volatile oil in Perilla leaves sold on the market and those planted in Baicao Garden of Hebei North University were determined by HPLC. The contents of total volatile oil were compared to evaluate whether the Perilla leaves planted in Baicao Garden of our school reached the market standard. Methods： Total volatile oils were extracted from two kinds of Perilla leaves by heating reflux method with 20 times anhydrous ethanol in 85℃ water bath for 1h. The content of Perilla aldehyde in total volatile oil was determined by HPLC， and the content of total volatile oil in two groups of Perilla leaves was calculated. Results： According to the linear regression equation， the content of Perilla aldehyde and total volatile oil in Perilla leaves sold on the market was 0.109% and 1.162%. The content of total volatile oil in Perilla leaves planted in our school was 0.102% and 1.090%. Conclusion： Although the content of volatile oil of Perilla leaves planted in Baicao Garden of our school is slightly lower than that of Perilla leaves sold on the market， it has reached the standard of market sale. Related Articles | Metrics | Comments（0）

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Clinical Efficacy of Jianzhong Yuyang Tablet in Treating Gastrointestinal Ulcer Bleeding and Its Influence on Gastroenteric Electroencephalogram DOU Hui-qiang ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 40-.   DOI: 10.3969/j.issn.2095-1396.2023.06.008 Abstract （4）      PDF（pc） （957KB）（2）       Knowledge map    Save Objective：To analyze the clinical efficacy of Jianzhong Yuyang tablet in the treatment of gastrointestinal ulcer bleeding and its influence on gastroenteric electroencephalogram. Methods： A total of 106 patients with gastrointestinal ulcer bleeding treated in our hospital from August 2021 to August 2023 were selected and divided into 2 groups according to different treatment methods. 52 cases in the comparison group were given conventional symptomatic treatment with Western medicine，and 54 cases in the observation group were given Jianzhong Yuyang tablet on the basis of the comparison group. The clinical efficacy，pH value of gastric fluid， hemoglobin （Hb）， electrogastroenterogram and serum inflammatory factors were compared between the two groups. Results： The total clinical effective rate of observation group（ 96.30%） was higher than that of comparison group（ 76.92%）（ P<0.05）. After treatment，the pH and Hb of gastric juice in observation group were higher than those in comparison group （P<0.05）. After treatment， the average amplitude of stomach body， gastric antrum， transverse colon， descending colon and rectum in observation group was higher than that in comparison group （P<0.05）. The levels of hypersensitive C-reactive protein （hs-CRP）， interleukin-10 （IL-10） and IL-8 in the observation group after treatment were lower than those in the control group （P<0.05）. Conclusion： Jianzhong Yuyang Tablet can effectively improve the symptoms of ulcer and bleeding in patients with gastrointestinal ulcer hemorrhage， increase Hb， regulate gastroenteric electroencephalogram， and reduce inflammation. Related Articles | Metrics | Comments（0）

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Cytotoxic Effect of BI-D1870 in N2a Cells YI Yan, SHANG Ya-zhen ACTA NEUROPHARMACOLOGICA    2023, 13 (3): 1-.   DOI: 10.3969/j.issn.2095-1396.2023.03.001 Abstract （2）      PDF（pc） （7366KB）（2）       Knowledge map    Save Objective: To investigate the cytotoxic effect of RSK inhibitor BI-D1870 in N2a and determine the concentration and time of cytotoxic effect. Methods: N2a cells were cultured in vitro, and the specific proteins NeuroD1, NeuN and DCX were identified by immunofluorescence. N2a cells were exposed to a series concentration of 10 μmol•L-1 to 80 μmol•L-1 BI-D1870 and a series duration of 4 h to 24 h. The morphology of N2a cell was observed with the inverted microscope, and the cell viability was measured with CCK-8 method, and lactate dehydrogenase (LDH) release of cell culture medium was assayed by pyruvate reduction method. Results: NeuroD1, NeuN and DCX were detected in N2a cells by immunofluorescence. Compared with the DMSO solvent control group, with the increase of BI-D1870 concentration and the exposure prolongation of BI-D1870, the damage degree of N2a cells gradually increased. The cell morphology changed abnormally, the cell viability decreased gradually (P<0.05) and LDH level in culture medium went up and then went down (P<0.05). Under the treatment of 40 μmol•L-1 for 12 h, the swelling of N2a cells was obvious, the refraction was reduced, the cell viability was about 60%, and the LDH release in culture medium was the highest. Conclusion: N2a cells have the characteristics of neuron. BI-D1870 has cytotoxic effect for N2a cells and the optimal cytotoxic condition is 40 μmol•L-1 for 12 h. Related Articles | Metrics | Comments（0）

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Efficacy of Mouse Nerve Growth Factor Combined with rt-PA in the Treatment of Acute Cerebral Infarction and Its Effect on Serum ET-1 and TXA2 Levels ZHANG Jin-yuan, LIANG Hua-min, MA Wei ACTA NEUROPHARMACOLOGICA    2023, 13 (6): 1-.   DOI: 10.3969/j.issn.2095-1396.2023.06.001 Abstract （5）      PDF（pc） （1024KB）（2）       Knowledge map    Save Objective：To investigate the clinical effect of mouse nerve growth factor （mNGF） combined with alteplase （rt-PA） in patients with acute cerebral infarction （ACI）. Methods： A total of 80 ACI patients were randomly divided into control group （40 cases） and observation group （40 cases）. The control group was treated with rt-PA thrombolytic therapy， and the observation group was treated with mNGF on the basis of rt-PA thrombolytic therapy. Both groups were treated for 14 days. Before and after treatment， the National Institutes of Health Stroke Scale （NIHSS） was used to evaluate the neurological function of the patients， the Barthel Index （BI） was used to evaluate the daily living ability of the patients， and the serum C-reactive protein （CRP）， erythrocyte sedimentation rate （ESR） and endothelin-1 （ET-1） and thromboxane A2 （TXA2） were detected. The differences in indicators and adverse reactions between the two groups were compared， and the incidence of adverse reactions in the two groups was counted. Results： Compared the total effective rate of the two groups， the observation group （95.00 %） was higher than the control group （80.00 %） ， and the difference was significant （P <0.05）. After treatment，compared with the control group， the NIHSS score was lower and the BI score was higher in the observation group （P <0.05）. The levels of serum CRP，ESR， ET-1 and TXA2 in the observation group were also significantly decreased （P <0.05）. There was no significant difference in the incidence of adverse reactions between the two groups （P >0.05）. Conclusion： mNGF combined with rt-PA in the treatment of ACI can improve the curative effect， promote the recovery of neurological function， and reduce the levels of serum ET-1 and TXA2. Related Articles | Metrics | Comments（0）

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Research Progress of Liquid Chromatography-Mass Spectrometry Technology in the Chemical Composition of Traditional Chinese Medicine YOU Si-han, YIN Hong-yan, YANG Song-rui, LIU Jia-meng, GUO Chun-yan ACTA NEUROPHARMACOLOGICA    2023, 13 (4): 8-.   DOI: 10.3969/j.issn.2095-1396.2023.04.002 Abstract （1）      PDF（pc） （990KB）（2）       Knowledge map    Save With the development of liquid chromatography-mass spectrometry (HPLC-MS), it is widely used in the analysis of traditional Chinese medicine. This technology not only provides fast chromatographic separation and short running chromatographic time, but also has high sensitivity and selectivity, which is helpful to identify the structure of various compounds of traditional Chinese medicine and analyze the mechanism of action of traditional Chinese medicine. This article reviews the research progress of liquid chromatography-mass spectrometry technology in the analysis of chemical components, quality control, and pharmacokinetics of traditional Chinese medicine in recent years, in order to provide new ideas and methods for the in-depth research and further development of traditional Chinese medicine. Related Articles | Metrics | Comments（0）

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Research Progress on Clinical Efficacy and Adverse Reactions of Esketamine HUANG Yu-li, LI Xiang-bo, YUAN li, TENG Jin-liang ACTA NEUROPHARMACOLOGICA    2023, 13 (4): 13-.   DOI: 10.3969/j.issn.2095-1396.2023.04.003 Abstract （1）      PDF（pc） （949KB）（2）       Knowledge map    Save Esketamine is a kind of pure dextroisomer of ketamine, compared with ketamine, the classic intravenous anesthetic, esketamine has a higher affinity to NMDA and opioid μ receptors, has stronger analgesic effect, and less respiratory depression, incidence of cardiovascular and psychiatric adverse reactions. It is also suitable for the clinical treatment of a variety of psychiatric disorders, such as epilepsy and depression. Effectively relieve patients' pain, improve patients' sleep quality, so as to achieve better treatment effect. It has gradually become an important non-opioid drug in perioperative low opioid program because of its strong analgesic effect, few adverse reactions, no influence of analgesic dose on respiratory and circulation stability, and little influence on mental state and cardiovascular system of patients. This article reviews the pharmacological properties, clinical applications and adverse reactions of esketamine. Related Articles | Metrics | Comments（0）