瓜子金皂苷己对鱼藤酮损伤PC12细胞的保护作用及其对CREB的影响

神经药理学报 ›› 2013, Vol. 3 ›› Issue (1): 12-16.

瓜子金皂苷己对鱼藤酮损伤PC12细胞的保护作用及其对CREB的影响

吴苗苗¹,苑玉和²,陈乃宏²

1. 河北北方学院药学系，张家口，075000，中国 2. 天然药物活性物质与功能国家重点实验室,中国医学科学院药物研究所, 北京，100050，中国

出版日期:2013-02-26 发布日期:2014-06-27
通讯作者: 陈乃宏，男，研究员，博士生导师；Tel.: +86-010-63165177，Fax: +86-010-63165177，E-mail: chennh@imm.ac.cn
作者简介:吴苗苗，女，助教，硕士；研究方向：神经药理学；Tel：+86-0313-4025966，E-mail：miaomiao2076924@126.com
基金资助:
国家自然科学基金（No.81274122、No.81102831、No.81173578），创新药物研究开发技术平台建设（No.2012ZX09301002-004、2012ZX09103101-006）

The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB

WU Miao-miao¹，YUAN Yu-he²，CHEN Nai-hong²

1. Department of Pharmacy Hebei North University，Zhangjiakou，075000，China 2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica，Chinese Academy of Medical Science and Peking Union Medical College，Beijing，100050，China

Online:2013-02-26 Published:2014-06-27
Contact: 陈乃宏，男，研究员，博士生导师；Tel.: +86-010-63165177，Fax: +86-010-63165177，E-mail: chennh@imm.ac.cn
About author:吴苗苗，女，助教，硕士；研究方向：神经药理学；Tel：+86-0313-4025966，E-mail：miaomiao2076924@126.com
Supported by:
国家自然科学基金（No.81274122、No.81102831、No.81173578），创新药物研究开发技术平台建设（No.2012ZX09301002-004、2012ZX09103101-006）

摘要/Abstract

摘要： 目的：观察瓜子金皂苷己（polygalasaponin F，PS-F）对鱼藤酮损伤的PC12细胞的保护作用及其对cAMP反应元件结合蛋白（cAMP response element binding protein，CREB）表达的影响。方法：以鱼藤酮损伤的PC12细胞为模型，通过倒置显微镜观察细胞形态；Caspase 3活性检测试剂盒测定Caspase3 活性；荧光素酶报告基因技术检测CREB表达的变化。结果：PC12细胞在4 μmol·L^-1鱼藤酮损伤后，细胞出现萎缩变圆，折光率降低，Caspase 3 活性显著升高，呈现凋亡的现象。不同浓度（0.1，1.0，10.0 μmol·L^-）瓜子金皂苷己，能够剂量依赖性的减轻对细胞形态的影响，降低Caspase3活性。荧光素酶报告基因标记显示，瓜子金皂苷己能够增加报告基因CREB的表达。结论：瓜子金皂苷己能够抑制鱼藤酮诱导的PC12细胞凋亡，其机制可能与增加CREB的表达有关，本研究提示，瓜子金皂苷己对神经系统具有一定的神经保护作用。

关键词: 帕金森病, 瓜子金皂苷己, 鱼藤酮, 凋亡, caspase 3, cAMP反应元件结合蛋白

Abstract: Objective：To investigate the effect of polygalasaponin F (PS-F) on the PC12 cells insulted by rotenone and the expression of CREB. Methods：The cell morphology was observed by inverted microscope. Caspase-3 activity was determined by caspase 3 activity assay kit. The expression of CREB was examined by luciferase reporter assay system. Results: When exposed to 4 μmol·L^-1 rotenone, a lot of PC12 cells were apoptotic, and the activity of caspase 3 was increased significantly. When treated with PS-F, the damage of the cells was reduced significantly and the caspase 3 activity was decreased significantly. PS-F could increase the expression of CREB. Conclusion: PS-F protects PC12 cells against apoptosis induced by rotenone and the neuroprotective effect may be related to increasing the expression of CREB.

Key words: Parkinson&, rsquo, s diseases, rotenone, apoptosis, caspase 3, CREB

吴苗苗，苑玉和，陈乃宏. 瓜子金皂苷己对鱼藤酮损伤PC12细胞的保护作用及其对CREB的影响[J]. 神经药理学报, 2013, 3(1): 12-16.

WU Miao-miao，YUAN Yu-he，CHEN Nai-hong. The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB[J]. Acta Neuropharmacologica, 2013, 3(1): 12-16.

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