关键词:

font-family:Helvetica, "> αfont-family:Times, ">- 突触核蛋白；帕金森病；慢病毒转染；内质网应激

Abstract:

Objective：Construction and identification of human SNCA and SNCAmu gene lentiviral vector，transfection into the 293T cells，and induce the endoplasmic reticulum stress. Methods：The target gene was amplified by PCR and inserted into lentiviral vector. The recombinant plasmid was constructed and packaged in 293T cells. The expression of GFP was observed by fluorescence microscope 24 hours after transfection. MTT method was used to detect the proliferation of cells in CON group，NC group，OE group and OEm group at different time points（1、2、3、4、5 days）. Western blotting measured the expression of caspase-12 induced by SNCA and SNCAmu. Results：Pgc-FU-SNCA-GFP and pGC-FU-SNCAmu-GFP were successfully constructed and transfected into 293T cells. At different time points（ 1、2、3、4、5），the proliferation of cells in CON group，NC group，OE group and OEm group has significance in statistics. SNCA and SNCAmu induced the expression of caspase-12 in 293T cells. Conclusion：SNCA induces caspase-12 of endoplasmic reticulum stress apoptosis pathway，which may be one of the pathogenic mechanisms of Parkinson’s disease.

Key words:

font-family:Times, "> SNCAfont-family:Helvetica, ">；Parkinson’s diseasefont-family:Helvetica, ">；lentiviral transfectionfont-family:Helvetica, ">；endoplasmic reticulum stress