神经药理学报

神经药理学报 ›› 2021, Vol. 11 ›› Issue (2): 11-14.DOI: 10.3969/j.issn.2095-1396.2021.02.003

• 研究论文 • 上一篇    下一篇

黄芪颗粒对老年痴呆性大鼠海马组织细胞凋亡的影响

叶三川   

  1. 厦门市仙岳医院，厦门，361012，中国

  • 出版日期:2021-04-26 发布日期:2021-04-26
  • 作者简介:叶三川，男，副主任医师；研究方向：临床精神病学、司法精神病学；E-mail：66712272@qq.com

Effect of Huangqi Granules on Apoptosis of Hippocampal Tissue in Alzheimer’s Rats

YE San-chuan   

  1. Xianyue Hospital of Xiamen City，Xiamen，361012，China

  • Online:2021-04-26 Published:2021-04-26
  • About author:叶三川，男，副主任医师；研究方向：临床精神病学、司法精神病学；E-mail：66712272@qq.com

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摘要：

目的：探讨黄芪颗粒对老年痴呆性大鼠海马组织细胞凋亡的影响。方法：选取健康的雄性SD 大鼠48只，随机分为4 组：对照组、模型组、黄芪颗粒组及吡拉西坦组，每组12 只。其中老年痴呆大鼠模型通过将β- 淀粉样蛋白1-40（β-amyloid protein 1-40，Aβ1-40）注射入大鼠脑室部建立。向黄芪颗粒组及吡拉西坦组分别灌胃给予溶于生理盐水的黄芪颗粒和吡拉西坦片，对照组与模型组均只给予等量生理盐水。经过30 天的治疗时间，通过Morris 水迷宫法测定每组SD 大鼠的穿台次数和逃避潜伏期来判断学习与记忆能力，通过Western Blot 实验测定各组大鼠海马组织细胞中抑凋亡蛋白B 淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2）及促凋亡蛋白Bcl-2相关X 蛋白（Bcl-2 associated X protein，Bax）和Caspase-3 含量水平，通过流式细胞术检测大鼠海马组织细胞凋亡情况。结果：与模型组大鼠相比，给予黄芪颗粒和吡拉西坦会使大鼠的穿台次数增加且逃避潜伏期缩短，结果具有统计学差异（P<0.05）。黄芪颗粒组及吡拉西坦组大鼠海马组织细胞中抑凋亡蛋白Bcl-2 水平明显上升，促凋亡蛋白Bax 和Caspase-3 水平显著下降。流式细胞术结果显示黄芪颗粒组细胞凋亡水平较模型组明显降低。结论：黄芪颗粒可以通过升高老年痴呆性大鼠海马组织细胞中Bcl-2 蛋白水平和降低Bax 和Caspase-3 蛋白水平，阻碍神经细胞凋亡进程，从而提高其学习与记忆能力。

关键词:

font-family:Helvetica, "> 黄芪颗粒, 老年痴呆, 海马组织, 凋亡

Abstract:

Objective：To investigate the effect of Huangqi granule on apoptosis of hippocampus in rats with Alzheimer’s disease. Methods：Forty-eight healthy male SD rats were selected and randomly divided into 4 groups：control group，model group，Huangqi granule group and piracetam group，with 12 rats in each group. The rat model of Alzheimer’s disease was established by injecting β-amyloid peptide 1-40 into the ventricle of the rat. Astragalus granule group and piracetam group were given Astragalus granules and piracetam tablets dissolved in normal saline，respectively. The control group and model group were given the same amount of normal saline. After 30 days of treatment，the Morris water maze method was used to determine the number of times of crossing and the escape latency of each group of SD rats to determine the learning and memory ability. Western Blot experiment was used to determine the apoptosisinhibiting protein Bcl-2 in the hippocampal tissue cells of each group，and the levels of proapoptotic proteins Bax and Caspase-3. The apoptosis of hippocampus was detected by flow  cytometry. Results：Compared with the model group of rats，the administration of Huangqi granules and positive drugs would increase the number of traversing and shorten the escape latency of rats. The results were statistically different（P<0.05）. The levels of anti-apoptotic protein Bcl-2 in the hippocampal tissue cells of the astragalus granule group and the piracetam group increased significantly，while the levels of pro-apoptotic proteins Bax and Caspase-3 decreased significantly. The results of flow cytometry showed that the apoptosis level of Huangqi granule group was significantly lower than that of model group. Conclusion：Astragalus granules can increase the level of Bcl-2 protein in the hippocampal tissue cells of Alzheimer’s disease rats and decrease the level of Bax and Caspase-3 protein to hinder the process of neuronal apoptosis，thereby improving the ability of learning and memory.

Key words:

font-family:Times, "> Huangqi granulesfont-family:Helvetica, ">, Alzheimer's diseasefont-family:Helvetica, ">, hippocampal tissuefont-family:Helvetica, ">, apoptosis

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