水飞蓟宾对丁酸钠引起的老龄鼠抑郁和焦虑样行为的影响

神经药理学报 ›› 2011, Vol. 1 ›› Issue (6): 17-21.

水飞蓟宾对丁酸钠引起的老龄鼠抑郁和焦虑样行为的影响

张岩¹，王加兴²，刘娜¹，卜玉洁¹，包金风¹

1．徐州医学院药学院药理学教研室，徐州，221004，中国
2．徐州医学院临床医学系，徐州，221004，中国

出版日期:2011-12-26 发布日期:2013-04-25
通讯作者: 包金风，女，博士，副教授，硕士生导师；研究方向：神经药理学；Tel：+86-0516-83262107，E-mail: jinfengbao66@126.com
作者简介:张岩，男，硕士生；研究方向：神经药理学；E-mail: yanzhang16@126.com 王加兴，上海市第六人民医院骨科研究生
基金资助:
江苏省2010年大学生实践创新训练计划，徐州医学院药学院研究生科研创新计划（No.2011YXKYCX0011）

Effects of Silibinin on Depression-like and Anxiety-like Behaviors Induced by Sodium Butyrate in Aged Mice

ZHANG Yan¹, WANG Jia-xing², LIU Na¹, BU Yu-jie¹, BAO Jin-feng¹

1. Dept of Pharmacology, Xuzhou Medical College, Xuzhou, 221004, China 2. Dept of Clinical Medicine, Xuzhou Medical College, Xuzhou, 221004, Chin

Online:2011-12-26 Published:2013-04-25
Contact: 包金风，女，博士，副教授，硕士生导师；研究方向：神经药理学；Tel：+86-0516-83262107，E-mail: jinfengbao66@126.com
About author:张岩，男，硕士生；研究方向：神经药理学；E-mail: yanzhang16@126.com 王加兴，上海市第六人民医院骨科研究生
Supported by:
江苏省2010年大学生实践创新训练计划，徐州医学院药学院研究生科研创新计划（No.2011YXKYCX0011）

摘要/Abstract

摘要： 目的: 研究丁酸钠对老龄鼠抑郁和焦虑样行为的影响及水飞蓟宾对其行为的干预作用。 方法: 8月龄昆明种小鼠随机分为对照组、丁酸钠组、丁酸钠+水飞蓟宾（0.1，0.2 g·kg^-1）组。通过悬尾实验、旷场实验、高架十字迷宫实验和明暗箱实验观察丁酸钠和水飞蓟宾对老龄鼠的行为学影响。 结果: 在悬尾实验中，丁酸钠组小鼠较对照组小鼠不动时间明显延长（P跨格次数显著减少（P<0.05）；水飞蓟宾组（0.2 g·kg^-1）小鼠较丁酸钠组小鼠跨格次数显著增加（P<0.05）。在高架十字迷宫实验中，丁酸钠组小鼠较对照组小鼠在闭臂停留时间显著升高（P<0.05）；水飞蓟宾组小鼠较丁酸钠组鼠在闭臂停留时间有降低趋势，但差异不显著（P>0.05）。在明暗箱实验中，丁酸钠组小鼠较对照组小鼠在明箱停留时间明显减少（P<0.05）；水飞蓟宾组（0.2 g·kg^{-1）小鼠较丁酸钠组小鼠在明箱停留时间明显增加（P。<0.05）} 结论: 丁酸钠可引起老龄鼠出现抑郁和焦虑样行为；水飞蓟宾能够明显改善丁酸钠引起的抑郁和焦虑样行为。<0.05）；水飞蓟宾组小鼠较丁酸钠组小鼠不动时间明显缩短（P<0.05）。在旷场实验中，丁酸钠组小鼠较对照组小鼠

关键词: 丁酸钠, 水飞蓟宾, 抑郁, 焦虑, 乙酰化

Abstract: Objective: To explore the behavioral dysfunctions induced by sodium butyrate in aged mice and the prophylactic effects of silibinin. Methods: Eight-month-old kunming mice were randomly divided into control group, sodium butyrate group and sodium butyrate plus Silibinin (0.1, 0.2 g·kg^-1) group. The behaviors of aged mice were examined via the tail suspension test, open field test, elevated plus maze test and light/dark box test. Results: In the tail suspension test,the immobility time of sodium butyrate group was significantly increased compared with control group (P<0.05), while silibinin significantly antagonized the increase in immobility time induced by solidum butyrate (P<0.05). In the open-field test, the total travel distance was significantly decreased in solidum butyrate group as compared with control group (P<0.05) and silibinin treatment reversed such a decrease. In the elevated plus maze test, time spent in closed arms after sodium butyrate treatment in mice was significantly increased as compared with the control group (P<0.05), and there was a trend that silibinin blocked such an increase but failed to reach statistical significance.In light/dark box test, the residence time in the light box for the mice in sodium butyrate group was significantly decreased than for those in control group (P<0.05). Silibinin (0.2 g·kg^-1) treated mice exhibited a significantly increased residence time of the light box compared with sodium butyrate group (P<0.05). Conclusion: Sodium butyrate can induce depression-like and anxiety-like behaviors. Silibinin can relieve those behavioral changes induced by sodium butyrate.

Key words: sodium butyrate, silibinin, depression-like, anxiety, acetylation

中图分类号:

R749
R964

张岩，王加兴，刘娜，卜玉洁，包金风. 水飞蓟宾对丁酸钠引起的老龄鼠抑郁和焦虑样行为的影响[J]. 神经药理学报, 2011, 1(6): 17-21.

ZHANG Yan, WANG Jia-xing, LIU Na, BU Yu-jie, BAO Jin-feng. Effects of Silibinin on Depression-like and Anxiety-like Behaviors Induced by Sodium Butyrate in Aged Mice[J]. Acta Neuropharmacologica, 2011, 1(6): 17-21.

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