大鼠大脑皮层星形胶质细胞体外培养方法建立及鉴定

神经药理学报 ›› 2011, Vol. 1 ›› Issue (6): 22-27.

大鼠大脑皮层星形胶质细胞体外培养方法建立及鉴定

范悦，颜勇，商亚珍

河北省中药研究与开发重点实验室，承德医学院中药研究所，承德， 067000，中国

出版日期:2011-12-26 发布日期:2013-04-25
通讯作者: 商亚珍，Tel：+86-0314-2290616，E-mail：shangyz1018@ yahoo.com.cn
作者简介:范悦，女，承德医学院2010届硕士研究生
基金资助:
河北省自然基金（No. C2009001007），河北省中医药管理局资助项目（No. 05027）

A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex

FAN Yue，YAN Yong，SHANG Ya-zhen

Hebei Provincial Key Laboratory of Traditional Chinese Medicine Research and Development, Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde 067000, China

Online:2011-12-26 Published:2013-04-25
Contact: 商亚珍，Tel：+86-0314-2290616，E-mail：shangyz1018@ yahoo.com.cn
About author:范悦，女，承德医学院2010届硕士研究生
Supported by:
河北省自然基金（No. C2009001007），河北省中医药管理局资助项目（No. 05027）

摘要/Abstract

摘要： 目的：大鼠大脑皮层星形胶质细胞的体外培养、纯化和鉴定。方法：1-3天新生大鼠大脑皮层细胞进行体外培养，通过差速贴壁和传代去除成纤维细胞和神经元等，采用免疫组化法检测星型胶质细胞特异性蛋白—胶质纤维酸性蛋白（Glial fibrillary acidic protein, GFAP）鉴定星型胶质细胞。结果：培养的细胞具有典型的星形胶质细胞形态且生长旺盛，第3代95%以上为星形胶质细胞，特异性抗原GFAP表达阳性。结论：该方法操作简单，可靠，建立了一种有效的星形胶质细胞体外培养方法。

关键词: 星形胶质细胞, 体外培养, 免疫组织化学, 胶质纤维酸性蛋白

Abstract: Objective: To establish a method for rat cerebral cortical astrocyte culture, purification and identification. Methods: The cerebral cortex cells of newborn rats (1-3 days old) were cultured. The fibroblast cells and neurons were removed through differential adhesion and passage. Glial fibrillary acidic protein (GFAP), an astrocyte specific protein, was used for identifying astrocytes by immunohistochemical method. Results: The cultured cells showed typical morphological features of astrocyles and grew well. The third generation of the cells comprised > 95% astrocytes. Conclusions:The method is a simple and reliable in vitro culture for obtaining high purity astrocytes from newborn rat cerebral cortex.

Key words: astrocytes, in vitro, immunohistochemical, glial fibrillary acidic protein

中图分类号:

R965.2

范悦，颜勇，商亚珍. 大鼠大脑皮层星形胶质细胞体外培养方法建立及鉴定[J]. 神经药理学报, 2011, 1(6): 22-27.

FAN Yue，YAN Yong，SHANG Ya-zhen. A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex[J]. Acta Neuropharmacologica, 2011, 1(6): 22-27.

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