叶酸对母婴分离引起小鼠成年焦虑样行为影响的初探

doi:10.3969/j.issn.2095-1396.2019.01.002

神经药理学报

叶酸对母婴分离引起小鼠成年焦虑样行为影响的初探

内蒙古医科大学基础医学院，呼和浩特，010110，中国

内蒙古医科大学基础医学院，呼和浩特，010110，中国

出版日期:2019-02-26 发布日期:2020-01-09
通讯作者: 包金风，女，教授，硕士生导师；研究方向：神经药理学；Tel：+86-0471-6657564，E-mail：jinfengbao66@126.com
作者简介:邸桐，女，硕士研究生；研究方向：神经药理学；E-mail：ditong123@126.com
基金资助:
国家自然科学基金项目（No.81541160），内蒙古自然科学基金项目（No.2015MS0312），内蒙古医科大学百万工程基金项目（No.YKD2014KJB002）

Preliminary Study on the Effect of Folic Acid on Anxiety-Like Behavior in Adult Mice Induced by Maternal Separation

DI Tong，ZHAO Zhi-jie，BAO Jin-feng

Basic Medical College，Inner Mongolia Medical University，Hohhot，010110，China

Online:2019-02-26 Published:2020-01-09
Contact: 包金风，女，教授，硕士生导师；研究方向：神经药理学；Tel：+86-0471-6657564，E-mail：jinfengbao66@126.com
About author:邸桐，女，硕士研究生；研究方向：神经药理学；E-mail：ditong123@126.com
Supported by:
国家自然科学基金项目（No.81541160），内蒙古自然科学基金项目（No.2015MS0312），内蒙古医科大学百万工程基金项目（No.YKD2014KJB002）

摘要/Abstract

摘要： 目的：探究叶酸对母婴分离小鼠的行为学影响。方法：将新生昆明小鼠分为对照组、模型组和叶酸组，待成年后进行行为学测试。结果：与对照组比较，模型组小鼠在旷场实验中的活动和探索行为减少，叶酸可以逆转这一状况；模型组小鼠在十字高架实验中的活动和探索行为下降，叶酸组与模型组比较未有统计学差异；在新事物识别实验中，模型组小鼠的识别率显著低于对照组，表现出识别新事物能力明显减弱，而叶酸可以明显改善母婴分离小鼠的识别新事物能力；在明暗箱实验中，母婴分离使小鼠更趋向在暗箱活动，而叶酸组小鼠不仅活动增加，且更倾向于在明箱活动。结论：母爱剥夺可引起小鼠成年期焦虑样行为和学习记忆能力下降，而叶酸可以改善这些状况。

关键词: 叶酸, 母婴分离, 焦虑样行为

Abstract:

Objective：To explore the effect of folic acid on maternal separation mice. Methods：The newborn Kunming mice were divided into control group，maternal separation group and maternal separation plus folic acid group. Behavioral tests were started at 10-week old mice. Results：Compared with control group，the results shown that the movement and exploration behavior of the maternal separation group were decreased in the open field test，however，folic acid could increasethe movement and exploration behavior of mice；In the elevated plus maze test，the maternal separation group had less activity and exploration behavior，but folic acid group no effect compared with the maternal separation group；In the new object recognition experiment，the recognition rate of the maternal separation group was significantly lower than control group，shown the cognition was impaired while folic acid could markedly improve the recognition caused by maternal separation procedure. In the light and dark box test，the maternal separation mouse tended to move in the black box，while folic acid improved activity and tended to explore the light box. Conclusion：The maternal separation induced the anxiety- depression-like behaviors and abnormalities of learning and memory in adult mice，while folic acid can ameliorate ability of cognitive and anxiety-like behavior.

Key words: folic acid , maternal separation , anxiety-like behavior

中图分类号:

R964

邸桐，赵智婕，包金风. 叶酸对母婴分离引起小鼠成年焦虑样行为影响的初探[J]. 神经药理学报, DOI: 10.3969/j.issn.2095-1396.2019.01.002.

DI Tong，ZHAO Zhi-jie，BAO Jin-feng. Preliminary Study on the Effect of Folic Acid on Anxiety-Like Behavior in Adult Mice Induced by Maternal Separation[J]. Acta Neuropharmacologica, DOI: 10.3969/j.issn.2095-1396.2019.01.002.

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叶酸对母婴分离引起小鼠成年焦虑样行为影响的初探

Preliminary Study on the Effect of Folic Acid on Anxiety-Like Behavior in Adult Mice Induced by Maternal Separation

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