Abstract: Objective: To establish two ulcerative colitis models in mice induced by oxazolone (OXZ) and 2，4，6-trinitrobenzene sulfonic acid (TNBS) respectively. Explore the influence of gender on mouse models. Methods: 30 KM healthy grown mice half male and half female were randomly divided into 6 groups which include group A (normal female group, n=5), group B (normal male group, n=5), group C (OXZ female model group, n=5), group D (OXZ male model group, n=5), group E (TNBS female model group, n=5), group F (TNBS male model group, n=5). Two OXZ model groups were coated on the skins with 2.4% OXZ solution (100% ethanol solutions) 0.2mL as skin sensitization in 2 days. At the sixth day, the two model group were given the enema(0.1mL 0.8% OXZ dissolved in 40% ethanol).The two TNBS model groups were administered with enema (1.8mg TNBS dissolved in 40% ethanol) at the day 1and day 6.Fast cannot help but water 24 hours before the operation of enema for all model groups mice. Normal group was not dealt with anything. The mental state, hair color, eating situation and the soil of mice were recorded and the activity index disease (DAI) values were evaluated one day after the molding. Then the mice were sacrificed and the colon was isolated out. The Colon Macroscopic Damage Index (CMDI) and the Colon Index (CI) were recorded. The level of the interleukin 4 and the tumor necrosis factor-α of the lesion site were detected. Result: Two kinds of mice ulcerative colitis model were induced successfully. The IL-4 and TNF-α levels of OXZ female model groups were significantly different from the OXZ male model group, but the TNBS model groups were not significant. The all indexes of model groups were significantly different from the normal group, except the CI of OXZ female group. Conclusion: both OXZ and TNBS could induce the ulcerative colitis model in mice which pathological characteristics were different. The gender factors have important influence on the induction of the ulcerative colitis model in mice.

Key words: ulcerative colitis, 2, 4, 6-trinitrobenzene sulfonic acid, oxazolone, gender