Abstract: Objective：To investigate the relationship among nerve growth factor (NGF), neural cell adhesion molecule (NCAM), CXC chemokine ligand 12(CXCL12), CXC chemokine receptor type 4 (CXCR4) and perineural invasion in salivary adenoid cystic carcinoma (SACC). Methods：We Choose 60 cases of SACC with complete clinical data, divided them into perineural invasion group and non perineural invasion group, marking every cases according to slide and made tissue assay. Using immunohistochemistry technique to detect the expression of NGF、NCAM、CXCL12/CXCR4 in the two group, we analysis the relationship  between factor and the clinical pathological parameters. Results：The ratio of NGF、CXCL12、CXCR4 protein’s positive expression in SACC perineural invasion group were more higher than that of the non perineural invasion group, there are magnificent difference between them (P＜0.05); there are no relationship between the expression of NGF、CXCL12、CXCR4 and patient's sex, histological types, tumor location and size(P＞0.05). There are positive relationship between the expression of NGF and CXCL12 (r=0.279, P＜0.05)and CXCR4 (r=0.451, P＜0.05), but there are no positive relationship between expression of CXCL12 and CXCR4 (P＞0.05). There are no expressions of NCAM in the two groups. Conclusion：NGF、CXCL12/CXCR4 all take part in the perineural invasion of SACC.

Key words: salivary adenoid cystic carcinoma (SACC), perineural invasion, tissue chip, NGF , NCAM , CXCL12