ACTA NEUROPHARMACOLOGICA ›› 2020, Vol. 10 ›› Issue (2): 15-18.DOI: 10.3969/j.issn.2095-1396.2020.02.004

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Determination of Two Chemical Constituents in Euonymus Maackii by HPLC

PANG Ce,ZHAO Peng-hui,ZHANG Guan-teng,ZUO Ya-qi,DING Meng,GUO Jing-jing,ZHEN Pan   

  1. 1. Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China
    2. Institute of Applied Chemistry,Hebei North University,Zhangjiakou,075000,China
  • Online:2020-04-26 Published:2020-04-26
  • Contact: 甄攀,女,教授,硕士生导师;研究方向:天然药物化学;E-mail:1006867119@qq.com
  • About author:庞策,男,硕士研究生;研究方向:天然药物化学;E-mail:871778521@qq.com
  • Supported by:
    河北省高等学校科学技术研究项目(No.ZD2016016),2018 年度河北北方学院项目(No.YB2018030)

Abstract:

Objective:To establish methods to determine contents of evonine (compound 1)and 6α,12-diacetoxy-1β,9α di (β-furancarbonyl-oxy)-4α-hydroxy-2β-2-methylbutanoyloxy-β-dihydroagarofuran (compound 2) simultaneously in Euonymus maackii by high performance liquid chromatography (HPLC),to determine the contents of two components in leaves,seeds and stems of Euonymus maackii. Methods:Extraction by petroleum ether,drying of petroleum ether,methanol dissolution,detection by HPLC. Syncronis C18 chromatographic column (250 mm×4.6 mm,5 μm) was adopted. The mobile phase was methanol-water(72∶28),the flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm,and the column temperature was 25℃. Results:The linear range of compound 1 and compound 2 was respectively 0.17~2.72 mg·mL-1(r1=0.999 7)、0.10~1.60 mg·mL-1( r2=0.999 5),the detection limit was respectively 10.3 μg·mL-1 and 13.8 μg·mL-1,the average recovery rate was 96.1% to 110.7%,99.6% to 103.5%,respectively. The content range of compound 1 in leaves of Euonymus maackii was 0.02~0.03 mg·g-1,and compound 2 was not detected. The contents of compound 1 and 2 in seeds ranged from 1.64 to 3.06 mg·g-1 and from 0.53 to 1.41 mg·g-1,respectively. Compounds 1 and 2 in stem were not detected. Conclusion:HPLC method is simple,fast,stable and accurate,which can be used to detect two chemical components in Euonymus maackii. Contents of two chemical compounds in leaves,seeds and stems of the same plant were different,and contents of two chemical components in the same parts of different plants were significantly different.

Key words: Euonymus maackii, evonine, dihydroagarofuran, HPLC