Protective Effect of 3'-daidzein Suffocates Sodium on Serum Deprivation-induced Hippocampal Neuronal Cell Damage

ACTA NEUROPHARMACOLOGICA ›› 2013, Vol. 3 ›› Issue (3): 15-18.

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Protective Effect of 3'-daidzein Suffocates Sodium on Serum Deprivation-induced Hippocampal Neuronal Cell Damage

ZENG Jing，XUE Jin-hua，LI Xiao，HUANG Zhi-hua

Gannan Medical University，Jiangxi Provincial Key Laboratory of Cerebral Vascular Pharmacology，Ganzhou，341000，China

Online:2013-06-26 Published:2014-06-27
About author:曾靖，药理学教授，硕导，江西省脑血管药理重点实验室主任；研究方向：心、脑血管药理；E-mail：zengjing61@hotmail.com
Supported by:
国家自然科学基金资助项目（No.81160399）；江西省高等学校科技落地计划项目（科学前沿，No.KJLD13085）

Abstract

Abstract: Objective: To study the protective effect of 3'-daidzein sulfonate sodium (3'-DSS) on hippocampal neuronal cell damage induced by serum deprivation. Methods: Monolayer cell suspension of newborn SD rats (0~24 h) hippocampus was cultured in vitro. As the cells matured, hippocampal neuronal cell injury model was made by using serum-free medium. After treated with 3'-DSS (3.0, 10.0, 30.0 µmol•L-1 of 3'-daidzein sulfonate sodium) and cultivate for 24 hours, the cell viability was detected by MTT assay, the cell shape changes were observed, the lactate dehydrogenase (LDH) activity of serum-removed hippocampal neurons was detected. Results: In the 3'-DSS treating groups, comparing with the control group, the boundary of hippocampal neuron cell membrane is clearer, cell bodies are normal, the retraction of synapses is relieved and the cell adherence to the wall was normal. The cell viability is increased in treated groups, while the LDH activity of serum-removed hippocampal neurons is decreased. The result of MTT revealed cell viability of serum-removed hippocampal neurons was increased in 3'-DSS treating group as compared to the control group. Conclusion: 3'-DSS shows significant protective effect on hippocampal neuronal cell damage induced by removing serum.

Key words: 3'-daidzein sulfonate sodium, hippocampal neurons, protective effect

CLC Number:

R964

ZENG Jing, XUE Jin-hua, LI Xiao, HUANG Zhi-hua. Protective Effect of 3'-daidzein Suffocates Sodium on Serum Deprivation-induced Hippocampal Neuronal Cell Damage[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(3): 15-18.

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Protective Effect of 3'-daidzein Suffocates Sodium on Serum Deprivation-induced Hippocampal Neuronal Cell Damage

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