ACTA NEUROPHARMACOLOGICA ›› 2013, Vol. 3 ›› Issue (3): 8-14.

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Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology

JIN Can,ZHANG Dan-shen   

  1. Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China
  • Online:2013-06-26 Published:2014-06-27
  • Contact: 张丹参,女,教授,博士生导师;研究方向:神经药理学;Tel:+86-0313-4029556,E-mail:zhangds2011@126.com
  • About author:金灿,女,硕士研究生;E-mail:cking5168@163.com
  • Supported by:

    国家自然科学基金项目(No.81274005),河北省自然科学基金项目(No.C2009001026)

Abstract: Objective: To implement uniform design-high throughput screening (UD-HTS) technology for screening the antioxidant and hippocampal neurons protection activities of radix salviae miltiorrhizae (RSM) ingredients using multifactor and multilevel combination design. Methods: Bioactive ingredients of RSM (7 liposoluble and 7 water-soluble) were combined with different ratios through uniform design. The bioactivity of the combination samples were assessed by anti-DPPH oxidation activity method (screening) and cytological experiment (rescreening). Results: Two optimal combination samples of the antioxidant and hippocampal neurons protection, A13 and PA, were identified. Conclusion: UD-HTS technology is suitable for multifactor and multilevel screening, which can provide rapid screening of optimal combinations.

Key words: radix salviae miltiorrhizae, uniform design-high throughput screening, 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl(DPPH), hippocampal neurons

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