Neuroprotective Effect of Mailuoning on MCAO Rats and Its Antioxidant Mechanism

doi:10.3969/j.issn.2095-1396.2017.04.001

Abstract

Abstract: Objective：To investigate the antioxidant mechanism of mailuoning on MCAO rats. Methods：18 adult SD rats were randomly divided into control group，MCAO group and mailuoning group，6 rats in each group. A rat middle cerebral artery occlusion （MCAO） model was established by suture method，ischemic 2 h and reperfusion 24 h. 2% 2， 3，5-triphenyltetrazolium chloride （TTC） was used to measure the cerebral infarct volume in rat. The content of reactive oxygen species （ROS） were determined by fluorescence spectrometry. The contents of malondialdehyde （MDA） and glutathione （GSH） in brain tissue were determined by absorbance method. The expression of cysteinyl aspartate specific proteinase （caspase-3），B-cell lymphoma-2 associated X protein （Bax），B-cell lymphoma-2 （Bcl-2） were measured by Western Blot. Results：Mailuoning could significantly decrease the neurological function of MCAO rats and decrease the volume of cerebral infarction. Mailuoning could inhibit the production of ROS and the formation of MDA，meanwhile increase the level of GSH in brain tissue. Mailuoning could also inhibit the expression of caspase-3，Bax，however，increase the expression of Bcl-2. Conclusion：The protective effect of mailuoning on MCAO rats may be related to its inhibition of oxidative stress and apoptosis.

Key words: mailuoning, ischemia-reperfusion injury, middle cerebral artery ocelusion(MCAO) model, oxidative stress

CLC Number:

R965

YANG Jie，LIU Fu-jia，TIAN Zi-xia，WANG Le-le，XIE Xin-mei，PANG Xiao-bin. Neuroprotective Effect of Mailuoning on MCAO Rats and Its Antioxidant Mechanism[J]. Acta Neuropharmacologica, 2017, 7(4): 1-7.

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