神经药理学报

神经药理学报 ›› 2013, Vol. 3 ›› Issue (6): 15-19.

• 实验方法学 • 上一篇    下一篇

链脲佐菌素诱导的大鼠糖尿病合并脑缺血损伤模型的建立

闫淼，王世蕊，李梅   

  1. 徐州医学院药学院；新药与临床应用实验室；药理学教研室，徐州，221000，中国
  • 出版日期:2013-12-26 发布日期:2014-06-27
  • 通讯作者: 李梅，女，硕士，副教授；研究方向：糖尿病合并脑缺血发病机制；E-mail: limeimail2002@163.com
  • 作者简介:闫淼，女，药理学硕士研究生；E-mail: ym853184333@qq.com
  • 基金资助:

    江苏省麻醉学重点实验室开放课题（No.KJS1107），江苏省高校研究生科研创新计划（No.CXZZ12_1000），徐州医学院药学院研究生科技创新计划（No.2011YXKYCX005），徐州医学院院长专项人才基金（No.2011KJ09），江苏省高校优势学科建设工程资助项目

A Model of Cerebral Ischemia Injury in Streptozotocin-Induced Diabetic Rats

YAN Miao, WANG Shi-rui, LI Mei   

  1. Key Laboratory of New Drags and Clinical Application, Dept of Clinical Pharmacology, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221000, China
  • Online:2013-12-26 Published:2014-06-27
  • Contact: 李梅，女，硕士，副教授；研究方向：糖尿病合并脑缺血发病机制；E-mail: limeimail2002@163.com
  • About author:闫淼，女，药理学硕士研究生；E-mail: ym853184333@qq.com
  • Supported by:

    江苏省麻醉学重点实验室开放课题（No.KJS1107），江苏省高校研究生科研创新计划（No.CXZZ12_1000），徐州医学院药学院研究生科技创新计划（No.2011YXKYCX005），徐州医学院院长专项人才基金（No.2011KJ09），江苏省高校优势学科建设工程资助项目

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摘要： 目的：建立稳定的大鼠糖尿病合并局灶性脑缺血损伤模型，并对有关建模成功的标准进行探讨与分析。方法：40只健康雄性SD大鼠随机分为正常饲养假手术组，正常饲养脑缺血再灌注损伤模型组，糖尿病脑缺血再灌注损伤模型组。糖尿病模型组一次性腹腔注射链脲佐菌素60 mg·kg -1建立I型糖尿病大鼠模型，分别在造模后1，4周检测各组大鼠的体质量和空腹血糖。在腹腔注射(ip) STZ后4周采用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，复灌注24 h，进行神经功能评分，测定脑梗死体积并计算梗死体积百分比。结果：腹腔注射链脲佐菌素4周后，糖尿病模型组与正常组大鼠相比，体质量显著下降（P<0.001），血糖值显著升高（P<0.001）。缺血复灌注损伤手术后，糖尿病模型组与正常组大鼠相比，再灌注损伤加重，脑梗死体积百分比显著增加（P<0.001）。模型成功率为73%。结论：本实验方法制备的大鼠糖尿病合并局灶性脑缺血损伤模型成功率较高，具有较好的重复性和稳定性。

关键词: 脑缺血, 糖尿病, 链脲佐菌素, 大鼠, 动物模型

Abstract: Objective: To establish a reliable model of focal cerebral ischemia injury in diabetic rats, and to explore the criteria for successful modeling. Methods: Forty healthy male SD rats were randomly divided into three groups: a normal sham-operation group, a normal cerebral ischemia-reperfusion injury group and a group of diabetes combined with cerebral ischemia-reperfusion injury. Type I diabetes was induced by an intraperitoneal injection of streptozotocin (STZ, 60 mg·kg-1). The body mass and the level of fasting blood glucose in each group were measured on Weeks 1 and 4 after STZ injection. A focal cerebral ischemia-reperfusion model was prepared by a thread occlusion method four weeks after STZ treatment. The scores of neurological function were assessed and the percentage of infarct volume were calculated after 30 min ischemia followed by 24 h of reperfusion. Results: The body mass (P<0.001) was reduced and the fasting blood glucose (P<0.001) was increased significantly in diabetic rats than those in normal rats four weeks after STZ injection. The percentage of infarct volume (P<0.001) was increased more significantly and the brain injury appeared more severe in diabetic rats than in normal rats after reperfusion. The success rate of this model was 73%. Conclusion: In this study, we developed a model of focal cerebral ischemia injury in diabetic rats that demonstrates a high success rate and good reliability and stability.

Key words: cerebral ischemia, diabetes mellitus, streptozotocin, rats, animal model

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