神经药理学报

神经药理学报 ›› 2024, Vol. 14 ›› Issue (4): 34-.DOI: 10.3969/j.issn.2095-1396.2024.04.006

• 研究论文 • 上一篇    下一篇

超声微泡辅助治疗对大鼠脑缺血再灌注损伤的治疗效果探讨

马洁华，王慕通，郭帆，刘旭，王悦瑶，赵宇，张辉   

  1. 1. 河北北方学院基础医学院，张家口，075000，中国 

    2. 河北北方学院第一临床医学院，张家口，075000，中国

  • 出版日期:2024-08-26 发布日期:2025-01-04
  • 通讯作者: 张辉，硕士生导师，教授；研究方向：脑损伤修复
  • 作者简介:马洁华，硕士研究生；研究方向：中枢神经损伤修复
  • 基金资助:
    河北北方学院校级青年基金项目（No.QN2018014）；河北省卫生计生委医学科学研究项目（No.20180809）；河北北方学院大学 生创新创业训练计划项目（No.XJ202216）

Exploration of the Therapeutic Effect of Ultrasound Microbubble- Assisted Therapy on Cerebral Ischemia-Reperfusion Injury in Rats

MA Jie-hua, WANG Mu-tong, GUO Fan, LIU Xu, WANG Yue-yao, ZHAO Yu, ZHANG Hui   

  1. 1. School of Basic Medicine, Hebei North University, Zhangjiakou, 075000, China 

    2. The First Clinical Medical College, Hebei North University, Zhangjiakou, 075000, China

  • Online:2024-08-26 Published:2025-01-04

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摘要：

目的：探讨超声微泡辅助治疗对大鼠脑缺血再灌注损伤的治疗效果。方法：采用线栓法制备大鼠脑缺血 再灌注模型，取造模成功的 40 只大鼠随机分为微泡组（超声辐照+ 微泡+ 溶栓）和对照组（超声辐照+ 溶栓） 各 20 例。 2 组大鼠均以 1 次·d-1 的频率接受治疗，持续治疗 15 d。溶栓治疗结束后，比较 2 组血栓清除评分、神经损伤 严重情况、脑组织含水率。结果：分组后 3、6 h 微泡组血栓清除评分高于对照组（P<0.05）；分组后 10、15 d 微泡组 改良神经功能缺损评分（modified neurological severity scores, mNSS）评分均低于对照组（P<0.05）；分组后 8 d， 微泡组脑组织含水率低于对照组（P<0.05）。 结论：超声联合微泡用于脑缺血再灌注大鼠，有助于血栓的清除，能 够减轻神经损伤及脑组织含水率。

关键词: 超声微泡, 脑缺血, 再灌注, 动物实验

Abstract:

Objective：To explore the therapeutic effect of ultrasound microbubble-assisted therapy on cerebral ischemia-reperfusion injury in rats. Methods: A rat cerebral ischemiareperfusion model was prepared by the wire thrombus method. 40 successfully modeled rats were randomly divided into a microbubble group (ultrasound irradiation+microbubble+thromb olysis) and a control group (ultrasound irradiation+thrombolysis), with 20 cases in each group. Both groups of rats received treatment at a frequency of 1 times·d-1 for 15 days. At the end of thrombolysis treatment, thrombus clearance scores, severity of neurological damage, and water content of the brain tissue were compared between the two groups. Results: Thrombus clearance score in the microbubble group was higher than that in the control group at 3 and 6 h after grouping (P<0.05). mNSS scores in the microbubble group were lower than that in the control group on the 10th and 15th day after grouping (P<0.05). The water content of brain tissue in the microbubble group was lower than that in the control group on the 8th day after grouping (P<0.05). Conclusion: Ultrasound combined with microbubbles for cerebral ischemia-reperfusion rats contributes to thrombus clearance and is able to attenuate neurological damage and brain tissue water content.

Key words: ultrasound microbubbles, cerebral ischemia, reperfusion, animal experiment

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