Total Saponins from Rhizoma Anemarrhenae Increase Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats /知母总皂苷改善糖尿病大鼠海马中乙酰胆碱酯酶的活性

摘要/Abstract

摘要： 目的：观察知母总皂苷（saponins from Rhizoma Anemarrhenae, TS）对糖尿病（diabetes mellitus, DM）大鼠海马中乙酰胆碱酯酶（acetylcholinesterase, AchE）活性的影响及可能机制。方法：雄性Sprague Dawley大鼠单次腹腔注射链脲霉素（streptozotocin, STZ, 55 mg?kg^-1）诱导DM大鼠模型后，按血糖值随机分为DM组和TS组，TS组灌胃给予TS（200 mg?kg^-1），每天一次，连续8周。测定海马中AchE活性、丙二醛（malondialdehyde, MDA）和还原型谷胱甘肽（reduced glutathione, GSH）水平，以及血清中MDA、GSH和葡萄糖水平。结果：相对于正常大鼠，8周病程的DM大鼠海马中AchE活性显著增加，给予TS能够改善上述变化。同时，DM大鼠海马和血清中MDA水平显著增加、GSH明显降低，而TS干预后能够明显逆转。此外，TS还能够明显降低DM大鼠的空腹血糖和增加体重。结论：知母总皂苷对糖尿病状态下海马中AchE活性具有一定的改善作用，这可能与其抗氧化应激和改善DM基本症状有关。

关键词: 糖尿病, 乙酰胆碱酯酶, 知母, 皂苷, 氧化应激, 降血糖

Abstract: Objective: To investigate the effects of total saponins from Rhizoma Anemarrhenae (TS) on acetylcholinesterase (AchE) activity in the hippocampus of streptozotocin-induced diabetic rats and their possible mechanisms. Methods: Diabetic rats were treated with TS (200 mg?kg^-1, ig.) for 8 weeks, and their blood glucose and body weight were measured monthly and weekly, respectively. The hippocampus was obtained for assays of AchE activity as well as levels of malondialdehyde (MDA) and reduced glutathione (GSH); blood was collected for assays of levels of MDA, GSH and glucose. Results: Compared with that of the age-matched normal rats, AchE activity was markedly increased in the hippocampus of diabetic rats, and TS treatment blocked such an increase. Meanwhile, diabetes significantly elevated the MDA level and reduced GSH level both in the hippocampus and the serum, and TS treatment significantly prevented such changes. In addition, TS significantly decreased the fasting blood glucose level and increased the body weights of diabetic rats. Conclusion: TShas an inhibitory effect on AchE activity in the hippocampus in diabetic rats, which is at least partly due to its antioxidant and anti-diabetic activity.

Key words: diabetes mellitus, acetylcholinesterase, Rhizoma Anemarrhenae, saponins, oxidative stress, blood glucose-lowering

中图分类号:

R965

ZHAI Yun-peng, ZHU Xia, LU Qian, WANG Jian-yun, YIN Xiao-xing, LIU Yao-wu. Total Saponins from Rhizoma Anemarrhenae Increase Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats /知母总皂苷改善糖尿病大鼠海马中乙酰胆碱酯酶的活性[J]. 神经药理学报, 2012, 2(1): 1-9.

ZHAI Yun-peng, ZHU Xia, LU Qian, WANG Jian-yun, YIN Xiao-xing, LIU Yao-wu. Total Saponins from Rhizoma Anemarrhenae Increase Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats[J]. Acta Neuropharmacologica, 2012, 2(1): 1-9.

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