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26 April 2024, Volume 14 Issue 2 Previous Issue   

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Xingnaojing Improves Neuroinflammation and Cognitive Dysfunction in Heatstroke-Induced Rats Based on TLR4 / NF-κB Signaling P athway ZHENG Li-li, LIU Mei-cen 2024, 14 (2):  1.  DOI: 10.3969/j.issn.2095-1396.2024.02.001 Abstract ( 49 )   PDF (2223KB) ( 14 )   Objective：To observe the effect of Xingnaojing on inflammatory cytokines in brain tissues in heatstroke-induced rats, and to explore the mechanism of cognitive improvement. Methods: Thirty-six Wistar rats were randomly divided into control group, model group, and Xingnaojing group. The rats were intervened with high temperature of (41±0.5) ℃ and high humidity of (75%±5%). The core temperature of the rats was monitored, when the temperature reach to 42 ℃ , the rats were treated with cooling administration, and the Xingnaojing group were administrated with drugs i.v. After 3 days of continuous intervention, water maze tests were performed, and then brains were taken for the expression of inflammatory cytokines and related proteins dectection. Results: The results of the study showed that Xingnaojing had a significant protective effect on cognitive dysfunction in heatstroke-induced rats. The intervention of Xingnaojing significantly reduced the expression of inflammatory cytokines in the rat brain,inhibited the activation of microglia in the brain, and improved neuronal apoptosis in the hippocampal CA1 area. In addition, Xingnaojing significantly reversed the increased expression of TLR4 protein, increased NF-κB phosphorylation and increased NLRP3 protein expression in the rat brain caused by heatstroke. Conclusion: Xingnaojing can improve cognitive dysfunction by suppressing the inflammatory response in the brain of heatstroke disease. Related Articles | Metrics

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Jiaotai Pill D rug-Containing Serum Ameliorates the Injury of SH-SY5Y Cells Induced by High Glucose/Palmitic Acid by Activating SIRT1 and Inhibiting Endoplasmic Reticulum Stress LI Pei-sen 2024, 14 (2):  7.  DOI: 10.3969/j.issn.2095-1396.2024.02.002 Abstract ( 48 )   PDF (3278KB) ( 14 )   Objective： To investigate the effect of Jiaotai Pill on SH-SY5Y cell injury induced by high glucose and high lipid. Methods： Twenty Sprague Dawley rats were randomly divided into control group and Jiaotai Pill group, with 10 rats in each group. The rats in the Jiaotai Pill group were given Jiaotai Pill solution 8.4 g·kg-1 by gavage, and the rats in the control group were given normal saline by gavage for 7 consecutive days to prepare blank serum and Jiaotai Pill serum. SH-SY5Y cells were cultured in vitro and treated with different proportions of serum for 24 hours. CCK-8 assay was used to detect the effect of serum containing Jiaotai Pill on the proliferation of SH-SY5Y cells. The cells were and divided into control group, low dose group and high dose group, SIRT1 inhibitor EX527 group according to the proportion of drug-containing serum and cultured with 25 mmol·L-1 high glucose and 200 μmol·L-1 palmitic acid or normal medium. Endoplasmic reticulum (ER) associated proteins [inositol-requiring enzyme 1α(IRE1α), phospho-inositol-requiring enzyme 1α(p-IRE1α), protein kinase R-like endoplasmic reticulum kinase (PERK), phospho-protein kinase R-like endoplasmic reticulum kinase (p-PERK), activating transcription factor 6(ATF6) and glucose regulated protein 78 (GRP78)、protein disulfide isomerase（PDI）] were detected by Western blot. Immunofluorescence was used to detect the expression of energy metabolism-related proteins (SIRT1、p-IRE1α、 p-PERK) in each group. Results： Compared with the control group, GRP78, the ratio of p-PERK to PERK and the ratio of p-IRE1α to IRE1α were significantly increased in the model group (P<0.05), SIRT1 and PDI were significantly decreased (P<0.05). Compared with the model group, the expressions of SIRT1 and PDI were up-regulated (P<0.05), and the expressions of GRP78, p-PERK/PERK,and p-IRE1α/IRE1α were down-regulated (P<0.05) in a concentrationdependent way in Jiaotai Pill low and high concentration groups.EX527 group significantly reversed the effect of Jiaotai pill(P<0.05). Conclusion： The drug-containing serum of Jiaotai Pill improve the high glucose/palmitic acid induced injury of SH-SY5Y cells by improving SIRT1 and inhibiting endoplasmic reticulum stress. Related Articles | Metrics

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Research on the Weight Loss Function and Mechanism of Tuckahoe Sawa DiarrheaTablets Based on Network Pharmacology and Molecular Docking Technology GAO Xiao-xin, CAO Chun-ran, LI Xue-mei 2024, 14 (2):  14.  DOI: 10.3969/j.issn.2095-1396.2024.02.003 Abstract ( 51 )   PDF (2472KB) ( 9 )   Objective：Network pharmacology and molecular docking technology combined with TCM dialectical formula were used to explore the weight loss effect and mechanism of tuckahoe sawa diarrhea tablets. Methods： According to the TCM syndrome type and medication experience, the active ingredients and targets of Poringze laxative tablets were collected through TCMSP database and UniProt database. DrugBank, OMIM database and GeneCards database were used to obtain obesity-related targets; The use of venn diagram to obtain the target of poria laxative tablets in the treatment of obesity; The STRING database constructs the target protein interaction network; Using Cytoscape 3.7.2 software to build a "drug-ingredient-key target" network; KEGG enrichment analysis of key targets was carried out by Metescape database. Finally use PyMOL 2.2 and Autodock Tools 1. 5. 6 software to carry out molecular docking of core targets and core components. Results： After topological analysis, a total of 52 key targets were obtained, and PTGS2 and PPARG may be important targets for the treatment of obesity by poria. In KEGG analysis, Insulin resistance, HIF-1 signaling pathway, PI3K-Akt signaling pathway, and Adipocytokine signaling pathway were closely related to the treatment of obesity. Molecular docking showed good results of quercetin docking with PTGS2 and PPARG, indicating that quercetin may be a key component of tuckahoe sawa diarrhea tablets in the treatment of obesity. Conclusion： tuckahoe sawa diarrhea tablets have the function of slimming in spleen deficiency and obstructive obesity, and realize the function of weight loss through multiple targets and multiple pathways. Related Articles | Metrics

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Mining of the Compatibility and Application Characteristics of Chinese Medicine Nidus Vespae Based on the Pharmacy Intelligence Network Database ZHANG Feng, ZHONG Juan, PAN Wen-bo 2024, 14 (2):  20.  DOI: 10.3969/j.issn.2095-1396.2024.02.004 Abstract ( 42 )   PDF (2704KB) ( 15 )   Objective: Based on data mining, the compatibility and application characteristics of formulas containing Nidus Vespae were discussed to provide references for rational drug use in honeycombs. Methods: Collect the prescriptions of Nidus Vespae in the Pharmacy Intelligence network database, construct Nidus Vespae prescription database, Microsoft Office Excel 2019, IBM SPSS Modeler 18.0, and IBM SPSS Statistics 23.0 were used to analyze the properties, flavors, Meridian Tropism, efficacy, and indications of Chinese herbal medicines. Association rules and cluster analysis were also conducted for high-frequency Chinese herbal medicines. Results: Among the 147 prescriptions that met the inclusion requirements, 353 Chinese herbs were used. Among the 1 597 cumulative uses, the most commonly used medicines were anti-toxin, insecticides, antipruritic drugs, heat-clearing drugs and drugs for promoting blood circulation and removing blood stasis, the medicine taste is more sweet, bitter and pungent, and mainly belongs to liver, stomach, spleen and heart meridian. The support and confidence of the 29 Association rules of 21 high-frequency Chinese herbal medicines were “Nidus Vespae-snake shed”,“ Nidus Vespae-angelica” and“ Nidus Vespae-pangolin”. the prescriptions of Nidus Vespae were mainly used to treat carbuncle, toothache, hemorrhoid, rheumatism and other diseases, 19 kinds of high-frequency Chinese herbs and 4 kinds of New Prescriptions were obtained by analyzing the prescriptions of treating carbuncle. Conclusion: In this study, data mining was used to analyze the compatibility characteristics and composition rules of formulas containing Nidus Vespae, which can provide references for clinical application and new drug development of Nidus Vespae. Related Articles | Metrics

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Exploring the Active Ingredients and Mechanism of Action of Paeoniae Radix Alba in the Treatment of Epilepsy Based on Network Pharmacology Methods XIA Lei, YOU Si-han, YIN Hong-yan, HAO Jun-rong, GUO Chun-yan 2024, 14 (2):  26.  DOI: 10.3969/j.issn.2095-1396.2024.02.005 Abstract ( 47 )   PDF (3233KB) ( 25 )   Objective：To explore the active ingredients and mechanism of action of Paeoniae Radix Alba in the treatment of epilepsy through network pharmacology. Methods: Obtain and screen the active ingredients and targets of Paeoniae Radix Alba from the Encyclopedia of Traditional Chinese Medicine database; Collect epilepsy related genes in GeneCards, OMIM, and DisGeNET databases. After taking the intersection of the active ingredient targets of Paeoniae Radix Alba and epilepsy disease targets, a protein interaction network was constructed using the STRING database. Then, visualization analysis was performed using Cytoscape 3.9.1 software. Core targets were screened using the Centiscape 2.2 plugin, and gene ontology functional annotation (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the David database, Finally, use Cytoscape 3.9.1 software to construct a drug component target network diagram. Results: There were 158 active ingredient targets in Paeoniae Radix Alba, 2398 disease targets in epilepsy, 38 intersection genes between Paeoniae Radix Alba and epilepsy, and GO enrichment analysis yielded BP136 entries, CC41 entries, MF49 entries. KEGG analysis revealed 106 pathways. Conclusion: Paeoniae Radix Alba may act on 38 targets such as TNF, Akt1, ALB, and HSP90AA1, inhibiting signaling pathways such as AGE-RAGE and NF kappa B, thereby treating epilepsy in terms of anti-inflammatory effects. Related Articles | Metrics

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Explore the Mechanism of Quercetin in the Treatment of Alzheimer's  Disease by Network Pharmacology and Molecular Docking WANG Tian-xu, LIU Ci, CUI Yong-yuan, ZHANG Xin, WU Miao-miao, SHEN Li-xia 2024, 14 (2):  31.  DOI: 10.3969/j.issn.2095-1396.2024.02.006 Abstract ( 87 )   PDF (4082KB) ( 27 )   Objective: To investigate the mechanism of quercetin in the treatment of Alzheimer's disease based on network pharmacology and molecular docking. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, PubChem database and Swiss Target Prediction platform were used to predict potential targets of quercetin. Potential targets for Alzheimer's disease were obtained through the Disgenet database. Common targets were obtained by intersection of potential quercetin targets and potential Alzheimer's disease targets through Venn diagram. The common targets were imported into DAVID database for screening at P<0.05, and gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed. The protein-protein interaction (PPI) network was constructed utilizing STRING database. The centiscape 2.2 plug-in of Cytoscape 3.6.0 software was used to analyze the PPI network, and the key target of quercetin in the treatment of Alzheimer’s disease was obtained. The top 5 key targets will be selected as the core targets in order of degree from largest to smallest, and the selected core targets will be subjected to molecular docking with SailVina final software. Results: There were 319 potential targets of quercetin in the three databases, 673 potential targets of Alzheimer's disease, 92 intersection targets, and 23 key targets screened out. The biological processes in GO analysis mainly included positive regulation of gene expression, positive regulation of apoptosis, response to exogenous stimuli, and negative regulation of apoptosis. KEGG pathway analysis mainly concentrated in IL-17, HIF-1, FoxO, TNF, PI3K-Akt, MAPK signaling pathway and so on. The average docking affinity between quercetin and core target molecules was -7.92 kcal·mol-1. Quercetin has good binding activity with the core targets IL-6, AKT, TP53, TNF and IL-1β. Conclusion: The potential targets and signaling pathways of quercetin in the treatment of AD have been found by network pharmacology and molecular docking techniques, which provided theoretical basis for subsequent experimental studies. Related Articles | Metrics

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Comparative Stud y of HER 2, RKIP and ERK Ex pression in Adenocarcinoma of Es ophagogastric J unction and D istal Gastric Adenocarcinoma FU Yu-tong, LI Hui-min, DONG Xin-yi, DU Shi-xuan, LOU Lei, CUI Jin-feng, WANG Juan, ZHANG Qing, SU Ling-rui, LI Yue-hong 2024, 14 (2):  38.  DOI: 10.3969/j.issn.2095-1396.2024.02.007 Abstract ( 44 )   PDF (4080KB) ( 7 )   Objective: To compare the expression of HER2, RKIP, and ERK in adenocarcinoma of esophagogastric junction (AEG) and distal gastric adenocarcinoma(DGA), and explore the differences in the pathogenesis of AEG and DGA. Methods: The expression of HER2, RKIP and ERK in AEG, DGA and normal gastric mucosal tissue were detected by immunohistochemistry, and the relationship between HER2, RKIP, and ERK with clinical pathological characteristics and their correlation were analyzed. Results: The positive expression rate of HER2 in AEG was higher than that in DGA, and the difference was statistically significant (P<0.05). The positive expression rates of RKIP in AEG and DGA were lower than that in normal gastric mucosal tissue, while the positive expression rates of ERK were higher than that in normal gastric mucosal tissue (P<0.05). In AEG, the positive expression rate of RKIP was lower in the high/moderate differentiation group and lymph node metastasis group, while the positive expression rate of ERK was higher in the low differentiation group, serosa invasion group, and lymph node metastasis group (P<0.05); HER2 expression was positively correlated with ERK expression (r=0.352, P=0.007), while RKIP expression was negatively correlated with ERK expression (r=-0.521, P=0.000).In DGA, the positive expression rate of RKIP was lower in the poorly differentiated group and lymph node metastatic group, while the positive expression rate of ERK was higher in the lymph node metastatic group (P<0.05); RKIP expression was negatively correlated with ERK expression (r=-0.287, P=0.021). Conclusion: The positive expression rate of HER2 in AEG is higher than that in DGA, and the high expression of ERK is more closely related to the clinicopathological characteristics of AEG patients. The expression of RKIP is closely related to the differentiation degree and lymph node metastasis of AEG and DGA. The expression of HER2, RKIP, and ERK in AEG and DGA is different, indicating that the regulatory mechanism of MAPK signaling pathway is not completely consistent in different locations of gastric adenocarcinoma. Related Articles | Metrics

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Research Progress of TRIM32 and Related Nervous System Diseases SUN Xiao-jing, MENG Xian-yong, YU Hui-jiao, DONG Xiao-hua 2024, 14 (2):  47.  DOI: 10.3969/j.issn.2095-1396.2024.02.008 Abstract ( 53 )   PDF (1032KB) ( 13 )   Tripartite motif proteins (TRIM) are a class of proteins involved in a wide range of biological processes, including cell growth and differentiation, development, apoptosis, immune response, and tumorigenesis. Among them, TRIM32 is an E3 ubiquitin ligase that belongs to the TRIM protein family. Although there are few studies on TRIM32, its role in maintaining the physiological function of the nervous system is extremely important. Therefore, this review aims to review the role of TRIM32 in neurological diseases. Related Articles | Metrics

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Research Progress of Curcumin Nano-Preparations YUAN Xin-ru, ZHANG Dan-shen 2024, 14 (2):  54.  DOI: 10.3969/j.issn.2095-1396.2024.02.009 Abstract ( 74 )   PDF (1011KB) ( 26 )   Curcumin (Cur) is a diketone compound extracted from the rhizomes of the genus turmeric, which has a wide range of pharmacological activities such as antioxidant, anti-inflammatory, anti-tumor, and neuroprotection, but has low solubility, short half-life, low bioavailability, difficult to penetrate the blood-brain barrier and other shortcomings limit its clinical application. In recent years, it has been prepared into liposomes, solid dispersions, polymer nanoparticles, polymer micelles, nanosuspensions, etc., which significantly improved the solubility, bioavailability and targeting of Cur, and improved drug activity. This article reviewed the nano-preparations of Cur, aiming to provide references for its further research and development. Related Articles | Metrics

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Research Progress on the Correlation Between Heart Failure and Insulin Resistance ZHANG Ze-peng, ZHANG Ai-ai, YANG Bo, LU Meng, LI Fang-jiang 2024, 14 (2):  59.  DOI: 10.3969/j.issn.2095-1396.2024.02.010 Abstract ( 59 )   PDF (1023KB) ( 17 )   In recent years, the treatment of cardiovascular diseases such as hypertension and coronary heart disease has made remarkable progress. However, the incidence of heart failure (HF) remains high and has become an important challenge in the cardiovascular field. At present, the urgent problem is how to effectively delay the occurrence and development of HF and reduce its fatality rate. Recent studies have shown that insulin resistance (IR) is closely related to HF. This article summarized the current research status of the correlation between heart failure and insulin resistance, and provided new ideas and strategies for the treatment of heart failure. The review is now reported as follows. Related Articles | Metrics