ACTA NEUROPHARMACOLOGICA ›› 2020, Vol. 10 ›› Issue (5): 1-4.DOI: 10.3969/j.issn.2095-1396.2020.05.001

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Application of Astragalus Injection in Intestinal Ischemia-Reperfusion Injury in Rats

HAO Zhan-wei,WU Xing-xing   

  1. The Second People Hospital of Lianyungang,Department of Pediatric Surgery,Lianyungang,222000,China
  • Online:2020-10-26 Published:2020-10-26
  • About author:郝占伟,硕士,副主任医师;研究方向:肝胆外科相关研究;E-mail:haozhanwei@163.com

Abstract:

Objective:To investigate the application of Astragalus injection in intestinal ischemia-reperfusion liver injury in rats and its influence on the expression levels of Bax and Bcl-2. Methods:9 healthy adult male Wistar rats were randomly divided into 3 groups:Sham group,model group (IIR group) and Astragalus injection group (Astragalus group),with 3 rats in each group. The 3 groups were divided into 3 different phases(each time phase was 1 rats) according to the time of reperfusion( 1,3,6 h). After the end of the experiment,the expression levels of Bax and Bcl-2 in the left lobe of rat liver were taken as the experimental standard,and the expression levels of Bax and Bcl-2 in all specimens were detected by MIAS-2000 medical image analysis and immunohistochemical method,and the apoptosis index(AI) of liver tissues was detected by the TUNEL technique. Results:Compared with Sham group,the expression levels of Bax and Bcl-2 in three different phases of IIR group and Huangqi group were significantly increased (P<0.05),while those of Bcl-2/Bax and Bcl-2/Bax in three different phases of IIR group and
Astragalus group were decreased (P<0.05). Compared with the IIR group,the reperfusion of bcl-2/Bax and Bcl-2 in the astragalus group at 1,3 and 6 h was significantly increased,while the level of Bax was decreased (P<0.05). There was no significant difference between different phases in the same group (P>0.05). Compared with Sham group,AI of IIR group and Huangqi group were significantly improved at different phases of reperfusion at 1,3 and 6 h (P<0.05). Compared with IIR group,AI of astragalus group in three different phases was significantly reduced (P<0.05). There were significant differences between IIR group and Astragalus group in different phases (P<0.05),and reperfusion was the highest for 6 h. Conclusion:IIR can lead to liver injury in rats,and the expression levels of Bcl-2 and Bax are increased. The mechanism of action of Astragalus injection may be to regulate the two expressions,alleviate the apoptosis of liver cells,and have a protective effect on the liver.

Key words: astragalus injection, intestinal ischemia-reperfusion in rats, liver injury