Abstract: Objective：To investigate the effect of cuprizone on the oligodendrocyte （OLs）related proteins. Methods: Mixed glial cells of cortices from neonatal rats（24 h after born） were primarily cultured for 7 d，and cells were purified by horizontal orbital shaking，differential adhesion and defined culture media. After three days，OLs were exposed to 25 nmol·L-1 cuprizone and further cultured for 3 days. Cells were observed by optical microscope and identified by immumofluorescence staining with MBP and DAPI. MBP，Fyn，Erk1/2 and other proteins were tested by Western Blot. Results：High purity mature OLs （specific marker MBP） were obtained. Compared to normal cells，the contents of MBP，Erk1/2 and p-Fyn （416 point） were significantly reduced in the cells exposed to Cuprizone. Conclusion: Cuprizone inhibits the expression of oligodendrocyte-associated proteins.

Key words: oligodendrocytes, cell culture, cuprizone