Protective Effects of Dendrobium Polysaccharides on Learning and Memory Deficits in Aging Mice Induced by D-galactose

Acta Neuropharmacologica ›› 2012, Vol. 2 ›› Issue (2): 1-6.

Protective Effects of Dendrobium Polysaccharides on Learning and Memory Deficits in Aging Mice Induced by D-galactose

WANG Ling-yi, HUANG Bin, YU Li-mei, WU Qin, ZHANG Feng, SHI Jing-shan

The Key Laboratory of Basic Pharmacology of Guizhou Province and Department of Pharmacology, Zunyi Medical College, Zunyi, 563099, China

Online:2012-04-26 Published:2013-11-01
Contact: 石京山，男，教授，博士生导师；研究方向：神经药理学；Tel:+86-0852-8609788, E-mail: shijs@zmc.edu.cn.
About author:王令仪，女，硕士研究生；研究方向：神经药理学；Tel:+86-0852-8609623, E-mail: sunny1984317@163.com
Supported by:
国家自然科学基金项目(No.30660206、30960447)，贵州省科技厅重点项目（No. 20051001），贵州省中医药管理局(No. C262 )

Abstract

Abstract: Objective: To investigate the effects of Dendrobium polysaccharides on D-galactose-induced learning and memory deficits in aging mice. Methods: Adult mice were given subcutaneous injection of D-galactose (120mg·kg^-1) daily for 60 d to produce aging-related syndrome, such as learning and memory deficits and alterations in tissue redox status. Dendrobium polysaccharides (80, 160 and 320 mg·kg^-1) were administrated by gavage daily for 60 d. Results:Dendrobium polysaccharides significantly ameliorated D-galactose-induced cognitive deficits, as evidenced by the significant performance improvement in the Morris water maze, step-down and step-through tests. Dendrobium polysaccharides also prevented the elevation of malondialdehyde (MDA) in the brain and plasma and restored the antioxidant enzyme activities to normal levels, including superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and Na⁺-K⁺-ATPase. Conclusions: Dendrobium polysaccharides is effective against D-galactose-induced aging and this effect appears to be due, at least in part, to its antioxidant activities.

Key words: Dendrobium polysaccharides, D-galactose, cognitive deficits, antioxidant activity

CLC Number:

R965

WANG Ling-yi, HUANG Bin, YU Li-mei, WU Qin, ZHANG Feng, SHI Jing-shan. Protective Effects of Dendrobium Polysaccharides on Learning and Memory Deficits in Aging Mice Induced by D-galactose[J]. Acta Neuropharmacologica, 2012, 2(2): 1-6.

References

[1] Fang Fang, Liu Gen-tao. A novel cyclic squamosamide analogue compound FLZ improves memory impairment in artificial senescence mice induced by chronic injection of D-galactose and NaNO2 [J]. Basic Clin Pharmacol Toxicol, 2007, 101(6):447-454.

[2] Vincenzo Solfrizzi, Alessia D'Introno, Anna Maria Colacicco, et al. Circulating biomarkers of cognitive decline and dementia [J]. Clin Chim Acta, 2006, 364(1-2):91-112.

[3] Yves SY Hsieh, Cheng Chien, Sylvian KS Liao, et al. Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense [J]. Bioorg Med Chem, 2008, 16(11):6054-6068.

[4] Ye Qing-hua, Qin Guo-wei, Zhao Wei-min. Immunomodulatory sesquiterpene glycosides from Dendrobium nobile [J]. Phytochemistry, 2002, 61(8):885-890.

[5] Zhang Xiu-li, Jiang Bo, Li Zhi-bo, et al. Catalpol ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by D-galactose [J]. Pharmacol Biochem Behav, 2007, 88(1): 64-72.

[6] Cui Xu, Zuo Ping-ping, Zhang Qing, et al. Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-a-Lipoic acid [J]. J Neurosci Res, 2006, 84(3):647-654.

[7] Zhou Li-bo, Du Li-yang, Dong Ai-mei. Effects of alcohol extract of Qizhou radix echinopsis on learning and memory in senile mice induced by D-galactose [J]. Chin Clin Rehab, 2005, 9(36):132-133.

[8] Ji Hun Park, Tae Saeng Choi. Polycystic ovary syndrome (PCOS)-like phenotypes in the d-galactose-induced aging mouse model [J]. Biochem Biophys Res Commun, 2012, 427(4):701-704.

[9] Zhang Xiu-li, Zhang Ai-hong, Jiang Bo, et al. Further pharmacological evidence of the neuroprotective effect of catalpol from Rehmannia glutinosa [J]. Phytomedicine, 2008, 15(6-7): 484-490.

[10] Udayan Dutta, Menashi A. Cohenford, Joel A. Dain. Nonenzymatic glycation of DNA nucleosides with reducing sugars [J]. Anal Biochem, 2005, 345(2):171-180.

[11] Naoyuki Sato, Shuko Takeda, Kozue Uchio-Yamada, et al. Role of insulin signaling in the interaction between Alzheimer disease and diabetes mellitus: a missing link to therapeutic potential [J]. Curr Aging Sci, 2011, 4(2):118-127.

[12] Gao Hui-ming, Hong Jaushyong. Why neurodegenerative diseases are progressive: uncontrolled inflammation drives disease progression [J]. Trends Immunol, 2008, 29(8):357–365.

[13] Shen Yu-xian, Xu Shu-yun, Wei Wei, et al. Melatonin reduces memory changes and neural oxidative damage in mice treated with D-galactose [J]. J Pineal Res, 2002, 32(3):173-178.

[14] Zhang Qing, Li Xue-kun, Cui Xu, et al. D-galactose injured neurogenesis in the hippocampus of adult mice [J]. Neurol Res, 2005, 27(5):552-556.

[15] Cui Xu, Wang Lu-ning, Zuo Ping-ping, et al. D-galactose- caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress [J]. Biogerontology, 2004, 5(5):317-325.

[16] Sung-liangYu, Shih-bin Lin, Yung-luen Yu, et al. Isochaihulactone protects PC12 cell against H(2)O(2) induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model [J]. Acta Pharmacol Sin, 2010, 31(12):1532-1540.

[17] Wang Ze-jian, Liang Cui-ling, Li Guang-mei, et al. Stearic acid protects primary cultured cortical neurons against oxidative stress [J]. Acta Pharmacol Sin, 2007, 28(3):315-326.

[18] Amy FT Arnsten, Min J Wang, Constantinos D Paspalas. Neuromodulation of thought: flexibilities and vulnerabilities in prefrontal cortical network synapses [J]. Neuron, 2012, 76(1):223-239.