The Effects of Stem-leaf Total Flavonoid on the Apoptosis Protein Expression and Ultrastructural Changes of Ischemia Reperfusion Damaged Neuron

Abstract

Abstract: Objective: To investigate the protective effect of Scutellaria baicalensis Stem-leaf Total Flavonoid on ischemia reperfusion damaged neurons in rats and its mechanism. Methods: the rat model of focal ischemia reperfusion was prepared after pretreatment with different doses of SSTF. The neurological function deficit was evaluated according to the Zea Longa 5-grade marking system; the brain infarction volume was measured by Triphenytetrazolium chloride（TTC）staining methods, while the immunohistochemical method was used to detect brain neuronal apoptotic gene expression. The ultrastructural damage changes of the cerebral neurons were observed under the electron microscope. Results: The neurological function deficit was alleviated by different doses of SSTF, with the Bcl-2 protein expression up-regulated and Bax expression down-regulated. The damage of organelles, especially the mitochondria of the neuron was significantly reduced. Conclusions: SSTF pretreatment can alleviate the neuronal damage incurred by ischemia reperfusion, demonstrating neuroprotective effect. The mechanism of such a neuroprotection may involve prohibition of the apoptosis of the neurons.

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Key words: scutellaria baicalensis stem-leaf total flavonoid (SSTF), ischemia reperfusion, neuron damage, Apoptosis, Bcl-2, Bax

CLC Number:

R961

ZHAO Shu-min, KONG Wei, ZHANG Shu-feng, CHEN Meng, ZHENG Xiao-ying, KONG Xiang-yu. The Effects of Stem-leaf Total Flavonoid on the Apoptosis Protein Expression and Ultrastructural Changes of Ischemia Reperfusion Damaged Neuron[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(5): 19-26.

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