Effects of Sargentodoxacuneata Total Phenolic Acids on Oxidative Stress Level and Energy Metabolism of Brain Tissues in Rats with Cerebral Ischemia Reperfusion Injury

doi:10.3969/j.issn.2095-1396.2019.01.001

Abstract

Abstract:

Objective：To investigate the effect of Sargentodoxacuneata total phenolic acids on oxidative stress level and energy metabolism in brain tissues of rats with focal cerebral ischemiareperfusion. Methods：The experiment was divided into 7 groups：sham operation group，model group，Nimodipine group （20 mg·kg-1），Naoluotong group （500 mg·kg-1），Sargentodoxacuneata total phenolic acids large，middle and small dose groups （300，150，75 mg·kg-1）. The drug was administered orally once a day for 7 days. One hour after the last administration，the model of focal cerebral ischemia-reperfusion in rats was established by thread embolization except in the shamoperated group. The other groups were withdrawn after 2 hours of ischemia to achieve reperfusion. After 24 hours of reperfusion，stained brain tissue with TTC，took photos and calculated infarct size. SOD，MDA levels and ATPase （Na+-K+-ATPase and Mg2+-ATPase） activity in brain tissue were also measured. Results：Compared with the model group，Nimodipine，Naoluotong and different doses of Sargentodoxacuneata total phenolic acids could reduce the area of cerebral infarction，reduce the MDA level，increased SOD level and ATP enzyme activity in brain tissue（ P<0.01 or P<0.05）. The effect of Sargentodoxacuneata total phenolic acids on cerebral ischemia-reperfusion in rats was dose-dependent. The effect of large dose Sargentodoxacuneata total phenolic acids on cerebral infarction area and SOD and MDA in brain tissue was similar to that of Naoluotong，but weaker than that of nimodipine. The improvement of ATP enzyme activity in brain tissue was similar to that of Nimodipine，but weaker than that of Naoluotong. Conclusion：Focal cerebral ischemia-reperfusion rat model was successfully reproduced. Sargentodoxacuneata total phenolic acids has a protective effect on focal cerebral ischemia-reperfusion rat model，has protective effect on cerebral ischemiareperfusion injury in rats，which may be related to its antioxidant activity and improvement of energy metabolism in brain tissue.

Key words: Sargentodoxacuneata total phenolic acids, thread method, cerebral ischemia reperfusion, anti-oxidation, energy metabolism

CLC Number:

R964

MIAO Ming-san，PENG Meng-fan，FANG Xiao-yan，JIA Jiao-jiao，BAI Ming. Effects of Sargentodoxacuneata Total Phenolic Acids on Oxidative Stress Level and Energy Metabolism of Brain Tissues in Rats with Cerebral Ischemia Reperfusion Injury[J]. Acta Neuropharmacologica, DOI: 10.3969/j.issn.2095-1396.2019.01.001.

References

[1]Mario Riverol, James Becker, Oscar L López, et al. Relationship between systemic and cerebral vascular disease and brain structure integrity in normal elderly individuals[J]. J Alzheimers Dis, 2015, 44(1) : 319-328.
[2]国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2015.
[3]马瑞丽, 张蓉, 徐秀泉, 等. 响应面分析法优化大血藤中总酚的超声提取工艺[J].中成药, 2013, 35(02): 404-407.
[4]闫安, 谢云亮. 当归多糖对脑缺血再灌注损伤大鼠脑组织氧化应激水平及炎症因子表达的影响[J].中国实验方剂学杂志, 2018, 24(02): 123-127.
[5]黄小平, 王蓓, 邱咏园, 等. 黄芪甲苷和三七的主要有效成分配伍对小鼠脑缺血再灌注后脑组织能量代谢的影响[J].中草药, 2014, 45(02): 220-226.
[6]Miao Ming-san, Cao Li-hua, Li Rui-qi, et al. Protective effect of chlorogenic acid on the focal cerebral ischemia reperfusion rat models[J]. Saudi Pharmaceutical J, 2017, 25(4): 556-563.
[7]陈佳佳, 冯红芳, 钟佳宏, 等. PDE4抑制剂FCPR16对局灶性脑缺血再灌注损伤大鼠的保护作用[J].神经药理学报, 2017, 7(02): 56.
[8]谢丽华, 赵毅锦, 林兴丽, 等. 中药治疗脑缺血的用药规律研究[J].中医药导报, 2017, 23(17): 38-40.
[9]项丽玲, 冯煜, 苗明三, 等. 玫瑰总黄酮对小鼠局灶性脑缺血模型的影响[J]. 中国现代应用药学, 2018, 35(1): 76-79.
[10]程晓, 张小莉, 白明, 等. 活血化瘀法、清热解毒法对脑缺血的防治特点及分析[J].中医学报, 2012, 27(05): 615-619.
[11]张莹莹,李诒光,季巧遇,等.大血藤现代研究进展[J].亚太传统医药, 2018, 14(11): 81-84.
[12]陈香君, 俸婷婷, 杨周洁, 等. 大血藤不同极性部位对H2O2损伤成骨细胞的保护作用研究[J].中国骨质疏松杂志, 2017, 23(03): 291-297.
[13]赵秀梅, 柯洪琴, 于慧斌. 大血藤药理作用与临床应用研究进展[J].中医药导报, 2014, 20(11): 41-43.
[14]B M, L BS, P MF, et al. Effect of Sargentodoxa cuneata total phenolic acids on focal cerebral ischemia reperfusion injury rats model[J].Saudi J Biological Sciences, 2019, 26(3): 569-576.
[15]黎丹东, 谢国旗, 高延玲, 等. 清脑片对大鼠脑缺血再灌注损伤的预防作用[J].中国药房, 2017, 28(16): 2198-2201.
[16]盛家琦. 钙通道阻滞剂尼莫地平在缺血性脑血管病的治疗进展[J].西部医学, 2013, 25(12): 1767-1769.
[17] Takashi Miyauchi, Enoch Wei, John T Povlishock. Evidence for the therapeutic efficacy of either mild hypothermia or oxygen radical scavengers after repetitive mild traumatic brain injury[J]. J Neurotrauma, 2014, 31( 8) : 773-781.
[18]苗明三, 彭孟凡, 刘保松, 等. 五味子粉对D-半乳糖致小鼠衰老模型的影响[J].中草药, 2018, 49(13): 3074-3081.
[19]杜云广, 曹欣欣, 王晓茹, 等. 牡荆苷对脑缺血再灌注大鼠脑损伤保护作用及其机制研究[J].神经药理学报, 2017, 7(01): 10-23.
[20]徐丽娟, 许斐, 臧春柳, 等. 复方菖蒲益智汤对脑缺血再灌注损伤小鼠脑组织Ca~(2+)-ATP酶及Na~+-K~+-ATP酶活性的影响[J].环球中医药, 2016, 9(11): 1289-1292.