Comparison in Tissue Distribution and Pharmacodynamics Effects between Three Chrysophanol Formulations in Mice with Cerebral Ischemia Reperfusion

Abstract

Abstract: Objective：To compare the pharmacodynamics and the tissue distribution of chrysophanol loaded polybutylcyanoacrylate nanocapsules，chrysophanol-hydroxypropyl-β-cyclodextrin inclusion complex，chrysophanol liposomes and DMF dissolved chrysophanol in mice with cerebral ischemia reperfusion. Methods：Using the cerebral ischemia reperfusion injury model，determined the chrysophanol content in blood，brain，heart，kidney，liver，spleen，lung of mice；measured the activity of malondialdehyde (MDA)，monoamine oxidase-B (MAO-B)，nitric oxide (NO)，nitric oxide synthase (NOS)，lipofuscin (LF)，acetylcholinesterase (AChE) in brain，the content of amino acid (AA) in brain and the content of lipofuscin (LF) in heart；observed the morphology of hippocampal. Results：The order of chrysophanol concentration in mouse tissues is：blood>brain>kidney>liver>heart>spleen>lung. Compared with chrysophanol DMF，chrysophanol concentrations of chrysophanol formulations (10.0，1.0，0.1 mg·kg^-1 dose groups) in brain，liver and blood tissues were significantly high (P<0.01~0.05) in a dose-dependent manner. At the same time，the aging-related enzymes’ activity and the pathological morphology in hippocampal area were improved to some extent. Conclusion：All the three chrysophanol formulations could enhance the anti-aging effects of chrysophanol in mice with cerebral ischemia reperfusion，with the possible mechanisms involving enhancement of chrysophanol content in brain，regulation of the activity of aging-related enzymes and improvement of the pathological morphology in hippocampi area.

Key words: chrysophanol, chrysophanol loaded polybutylcyanoacrylate nanocapsules, chrysophanol-hydroxypropyl-beta, -cyclodextrin inclusion complex, chrysophanol liposomes, cerebral ischemia reperfusion, tissue distribution, aging-related enzymes, tissue pathology

CLC Number:

R965
R361

LI Chao, ZHANG Li. Comparison in Tissue Distribution and Pharmacodynamics Effects between Three Chrysophanol Formulations in Mice with Cerebral Ischemia Reperfusion[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(4): 16-25.

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