Abstract:

Objective：To explore the effect of sea buckthorn oil on cell proliferation and apoptosis of human gastric cancer cells HGC-27. Methods：HGC-27 cells were treated with sea buckthorn oil. The cell viability was measured by CCK-8 kit. Western blotting was used to detect the protein expression of p53，p21 and Bcl-2. Results：Cell viability tests results showed that there is no effect on HGC-27 cell viability when DMSO content in cell culture medium is less than or equal to 0.1%. HGC-27 cells were treated with sea buckthorn oil dissolved in DMSO. The proliferation of HGC-27 cells was inhibited by sea buckthorn oil at the concentration of 5，10，20，

40 mol·L^-1（P<0.05）. Compared with control group，the expression level of p53 in three different concentration of sea buckthorn oil （10，20，40 mol·L^-1） group were significantly increased（P<0.05）. The expression level of Bcl-2 was significantly decreased and p21 was significantly increased in the concentration of sea buckthorn oil （5，10，20，40 mol·L^-1） group. Conclusion：Sea buckthorn oil can inhibit HGC-27 proliferation and promote apoptosis by p53 signaling pathway，and has anti-cancer effect.

Key words: HGC-27, P53, sea buckthorn oil, P21