ACTA NEUROPHARMACOLOGICA ›› 2020, Vol. 10 ›› Issue (5): 14-17.DOI: 10.3969/j.issn.2095-1396.2020.05.004

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Determination of Atractone in Tangpingjian by Gas Chromatography

TIAN Jia-ming,YU Xiao-hong,WANG Yin-yue,WANG Xu,WANG Zhi-bao,WANG Jin,LU Jin-ming   

  1. 1. Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China
    2. Hebei Provincial Key Laboratory of Neuropharmacology,Zhangjiakou,075000,China
    3. Hebei Building Engineering School,Baoding,071000,China
  • Online:2020-10-26 Published:2020-10-26
  • About author:田嘉铭,男,硕士,工程师;研究方向:主要从事中药制剂质量控制研究;E-mail:1750345551@qq.com
  • Supported by:
    张家口市科技局项目(No.1411063C)

Abstract:

Objective:To establish a gas chromatography method for the determination of atractylone in TangpingJian. Method:PEG-20M capillary column (30 m×0.25 mm×0.25 μm) was used as the chromatographic column. The temperature was programmed with the inlet temperature of 290 ℃ and detector temperature of 300 ℃,and the detector was hydrogen flame ionization detector (FID). The carrier gas was nitrogen with the flow rate of 1 mL·min-1, and naphthalene was used as the internal standard substance. Results:The separation of the atractylone and the internal standard substance was good,and the average recovery rate and RSD was 100.1% and 2.64%,respectively. The atractylone content in the Tangpingjian was 1.13 mg·g-1. Conclusion:The established gas chromatographic method is simple,highly sensitive and accurate. It can be used for the detection of atractylone content in TangpingJian.

Key words: tangpingjian, atractylone, determination, gas chromatography