ACTA NEUROPHARMACOLOGICA ›› 2020, Vol. 10 ›› Issue (1): 1-4.DOI: 10.3969/j.issn.2095-1396.2020.01.001

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Expressions of Slit2/Robo1 in Rats with Cerebral Infarction and Changes after Intervened by Urinary Kallidinogenase

LIU Hai-yan,QIAN Qi,ZHENG Xian-zhao,LV Hai-dong   

  1. Department of Neurology,People’s Hospital of Jiaozuo,Jiaozuo,454000,China
  • Online:2020-02-26 Published:2020-02-26
  • Contact: 吕海东,男,主任医师,硕士生导师;E-mail:hnlhd666@163.com
  • About author:刘海燕,E-mali:13253621995@163.com

Abstract:

Objective:To explore the relationship between Slit2/Robo1 signaling and investigate the expression of Slit2&Robo1 in rat cerebral infarction models and Urinary Kallidinogenase( UK) intervention. Methods:Fifty-four male Sprague Dawley rats were assigned into sham group (SG),model group (MG) and Urinary Kallidinogenase group (UKG) randomly. The groups were further randomized into three subgroups:1 d,3 d,and7 d after infarction occurred. Cerebral ischemia model was established via the suture method. UKG were injected UK via tail vein and the MG received equal volume of saline. Western-blot was used to analyze the level of Slit2 and eNOS at different time points and RT-PCR was used to detect the expression of Robo1 mRNA. Results:The expression of Slit2 and eNOS was significantly increased in the MG and UKG,meanwhile,it was positively correlated with eNOS. In the MG and UKG,the levels of Robo1 were significantly decreased in the early period and the expression remained suppressed
until 3 d after occlusion,not yet reached the level of SG by 7 d,the difference was statistically significant. Conclusions:The expression of Slit2 protein in rats after focal cerebral infarction is increased,and Slit2 may participate in the process of angiogenesis after cerebral infarction in rats. Urinary Kallidinogenase may promote the process of angiogenesis in rats after cerebral infarction by increasing Slit2 / Robo1.

Key words: focal cerebral infarction, Slit/Robo pathway, angiogenesis, Urinary Kallidinogenase