母鼠高脂饮食易化雄性子鼠对血管紧张素Ⅱ的升压作用

doi:10.3969/j.issn.2095-1396.2016.03.002

神经药理学报 ›› 2016, Vol. 6 ›› Issue (3): 8-16.DOI: 10.3969/j.issn.2095-1396.2016.03.002

母鼠高脂饮食易化雄性子鼠对血管紧张素Ⅱ的升压作用

房志琴，王亚玲，彭伟

1.河北北方学院，张家口，075000，中国
2.河北北方学院附属第一医院心血管内科，张家口，075000，中国

出版日期:2016-06-26 发布日期:2016-05-10
通讯作者: 王亚玲，女，教授，研究生导师，心内科副主任；研究方向：心脏电生理起搏器彭伟，男，教授，研究生导师；研究方向：高血压
作者简介:房志琴，女，河北北方学院2014级研究生；研究方向：心血管内科； E-mail：949774981@qq.com
基金资助:
河北省2015年研究生创新资助项目（No.204）

Maternal High Fat Diet Facilitation The Pressor Effect Of Male Offsprings To Angiotensin II

FANG Zhi-qin, WANG Ya-ling, PENG Wei

1. Hebei North University, Zhangjiakou, 075000, China 2. First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, China

Online:2016-06-26 Published:2016-05-10
Contact: 王亚玲，女，教授，研究生导师，心内科副主任；研究方向：心脏电生理起搏器彭伟，男，教授，研究生导师；研究方向：高血压
About author:房志琴，女，河北北方学院2014级研究生；研究方向：心血管内科； E-mail：949774981@qq.com
Supported by:
河北省2015年研究生创新资助项目（No.204）

摘要/Abstract

摘要： 目的：验证母鼠高脂饮食通过上调脑肾素-血管紧张素系统（renin-angiotensin-system，RAS）表达，来增加成年雄性子鼠对血管紧张素Ⅱ（Angiotensin II，AngⅡ）敏感性，并且易化AngⅡ引起的高血压。方法：雌性成年SD鼠随机分成高脂组和标准饮食组，与健康成年雄鼠交配，高脂饮食组孕前4周、孕期和哺乳期进行高脂饮食，普通饮食组各时期均普通饮食喂养，所有雄性子代分为两组：高脂饮食子代组（OHH）和正常饮食子代组（OCC），所有子鼠断奶后均标准饮食。子代出生当天称体质量，3周龄时检测脑终板（lamina terminalis，LT）和室旁核（paraventricular nucleus，PVN）中RAS成分。10周龄时脑室插管检测中枢对AngⅡ敏感性，并慢性灌流AngⅡ观察升压应答。结果：高脂饮食较正常饮食组脑中RAS成分表达增加，对急性脑室注射AngⅡ后的升压效应更敏感，对慢性灌流AngⅡ的升压应答更明显，且慢性灌流AngⅡ后RAS成分表达增加更明显。结论：母鼠高脂饮食通过上调雄性子鼠脑肾素-血管紧张素系统（RAS）表达，使中枢对血管紧张素Ⅱ（Ang Ⅱ）敏感性增加，并且易化AngⅡ引起的高血压。

关键词: 母鼠高脂饮食, RAS系统, 高血压

Abstract: Objective: To verify maternal rat high fat diet by up-regulating brain renin-angiotensin-system (RAS) to add the sensitivity of adult offspring to angiotensin II (Ang II), facilitation of Ang II induced hypertension. Methods: Adult female SD rats were randomly divided into high fat diet group and standard group, and healthy adult male mating, high fat diet group at 4 weeks before pregnancy, pregnancy and lactation were high fat diet, normal diet group were fed with normal diet in each period, all offspring were divided into two groups: high-fat diet group offspring (OHH) and normal diet group (OCC), the offspring of all offspring were the standard diet after weaning. Measure birth weight of offspring, 3 weeks of age to detect brain the RAS components in lamina terminalise (LT) and paraventricular nucleus (PVN). At 10 weeks of age, the sensitivity of the central nervous system to Ang II was detected in the cerebral ventricle, and the pressor response was observed in Ang II. Results: High fat diet increased the expression of RAS in the brain than the normal diet group, more sensitive pressor effection to intraventricular injection Ang II, and more obvious pressor response to the chronic perfusion Ang II, and the expression of RAS was significantly increased after perfusion. Conclusion: Maternal high fat diet increased the sensitivity of the central nervous system to angiotensin II (Ang II), and facilitated the hypertension induced by Ang II, by means of raising the expression of the ranin-angiotensin-aldosterone-system (RAAS).

Key words: maternal high fat diet, renin-angiotensin-system, hypertension

房志琴, 王亚玲, 彭伟. 母鼠高脂饮食易化雄性子鼠对血管紧张素Ⅱ的升压作用[J]. 神经药理学报, 2016, 6(3): 8-16.

FANG Zhi-qin, WANG Ya-ling, PENG Wei. Maternal High Fat Diet Facilitation The Pressor Effect Of Male Offsprings To Angiotensin II[J]. ACTA NEUROPHARMACOLOGICA, 2016, 6(3): 8-16.

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母鼠高脂饮食易化雄性子鼠对血管紧张素Ⅱ的升压作用

Maternal High Fat Diet Facilitation The Pressor Effect Of Male Offsprings To Angiotensin II

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