神经药理学报

神经药理学报 ›› 2016, Vol. 6 ›› Issue (3): 1-7.DOI: 10.3969/j.issn.2095-1396.2016.03.001

• 研究论文 •    下一篇

组胺对斑马鱼幼鱼致畸作用及抑制运动功能的研究

黄术兵，汪林芳，徐一达，崔春，申延琴   

  1. 江南大学无锡医学院，神经科学中心，无锡，214122，中国
  • 出版日期:2016-06-26 发布日期:2016-05-10
  • 通讯作者: 申延琴，教授，硕士研究生导师；研究方向：神经退行性疾病；Tel&Fax：+86-510-85197596，E-mail：shenyanqin@jiangnan.edu.cn
  • 作者简介:黄术兵，男，硕士研究生；研究方向：神经退行性疾病；E-mail：joaisheng@163.com汪林芳，女，硕士研究生；研究方向：神经退行性疾病；E-mail：18226622733@163.com
  • 基金资助:

    国家自然科学基金项目（No.81650007）

Study of Teratogenicity and Inhibition of Motor Function of Histamine in Development of Zebrafish Larvae

HUANG Shu-bing，WANG Lin-fang，XU Yi-da，CUI Chun，SHEN Yan-qin   

  1. Neuroscience Center，Jiangnan University Wuxi Medical School，Wuxi，214122，China
  • Online:2016-06-26 Published:2016-05-10
  • Contact: 申延琴，教授，硕士研究生导师；研究方向：神经退行性疾病；Tel&Fax：+86-510-85197596，E-mail：shenyanqin@jiangnan.edu.cn
  • About author:黄术兵，男，硕士研究生；研究方向：神经退行性疾病；E-mail：joaisheng@163.com汪林芳，女，硕士研究生；研究方向：神经退行性疾病；E-mail：18226622733@163.com
  • Supported by:

    国家自然科学基金项目（No.81650007）

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摘要： 目的：研究水环境中组胺对斑马鱼幼鱼发育和运动功能的影响。方法：将斑马鱼新生受精的胚胎孵育并饲养在梯度浓度组胺的水环境中（组胺浓度分别为0、1.25、2.5、5、10和20 mmol•L-1，0 mmol•L-1组作为空白组），分别在胚后第3天（day post-fertilization, dpf）和第4天统计并计算斑马鱼胚胎的孵化率及幼鱼的畸形率，并采集幼鱼的畸形形态；实时定量荧光PCR方法（quantitative real-time polymerase chain reaction, qRT-PCR）检测组胺受体H1R、H2R和H3R的表达；行为学追踪的方法检测0、5和20 mmol•L-1组未畸形斑马鱼幼鱼的运动能力。结果：水环境中组胺浓度越高，斑马鱼畸形率越高,但不影响孵化率。水环境组胺主要引起斑马鱼幼鱼发育过程中心脏器官异常发育，导致幼鱼出现弯尾短尾等及畸形。同空白组相比，高浓度组胺（5和20 mmol•L-1）组的斑马鱼幼鱼运动水平显著下降（P < 0.01）。斑马鱼幼鱼组胺H2R和H3R受体表达显著下调（P < 0.01 或 P < 0.001）。结论：环境组胺对斑马鱼发育具有致畸作用，并且会抑制幼鱼的运动功能。

关键词: 组胺, 斑马鱼, 发育, 运动功能

Abstract: Objective: To investigate the effects of histamine in water environment on the development of zebrafish larvae and its locomotor function. Methods: Newborn fertilized embryos of zebrafish were incubated and fed in gradient concentration of histamine solution (histamine concentrations were 0, 1.25, 2.5, 5, 10 and 20 mmol•L-1, respectively, 0 mmol•L-1 group as blank group). The hatching rate of zebrafish embryos and deformity rate of zebrafish larvae were calculated on the 3 day post-fertiliaztion (dpf) and 4 dpf respectively. The photos of deformed morphology of zebrafish larvae were collected at 4 dpf. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of histamine H1R, H2R and H3R type receptors. Swimming tracing was used to detect the motor function of non-deformed zebrafish larvae in 0, 5 and 20 mmol•L-1 group. Results：The aberration rate of zebrafish larvae increased with the higher concentration of histamine in water while the hatching rate was not affected in each group. Histamine treatment in water induced abnormal development of heart as well as body abnormalities of curving and short tail during the development of zebrafish larvae. Compared to the blank group, the motor function of zebrafish larvae in 5 and 20 mmol•L-1 group was significantly inhibited (P <0.01). The expression of H2R and H3R receptors in 5 and 20 mmol•L-1 was significantly decreased (P < 0.01 or P < 0.001). Conclusion: Histamine treatment in water environment leads to deformation of zebrafish larvae during development and inhibits motor function of larvae.

Key words: histamine, zebrafish（Danio rerio）, development, locomotor function