神经药理学报

神经药理学报 ›› 2023, Vol. 13 ›› Issue (2): 24-.DOI: 10.3969/j.issn.2095-1396.2023.02.004

• 研究论文 • 上一篇    下一篇

妊娠高血压疾病发生发展潜在生物信息学机制初探

高静，张帅   

  1. 河北北方学院附属第二医院，张家口，075100，中国

  • 出版日期:2023-04-26 发布日期:2023-11-30

Preliminary Study on the Potential Bioinformatics Mechanism of the Occurrence and Development of Hypertensive Disorder Complication Pregnancy

GAO Jing, ZHANG Shuai   

  1. Second Affiliated Hospital, Hebei North University, Zhangjiakou, 075100, China
  • Online:2023-04-26 Published:2023-11-30

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摘要：

目的：基于生物信息学初步探讨影响妊娠高血压疾病（hypertensive disorder complication pregnancy, HDCP）发生发展的潜在机制。方法：选取GEO数据库中GSE190639和GSE206763两个数据集，分别通过GEO2R在线平台筛选差异基因及差异miRNA，绘制火山图、PPI图和KEGG富集图并进行相应的分析。选取河北北方学院附属第二医院2022-01～2022-12月接受治疗的妊娠高血压疾病患者（病例组）和健康妊娠者（对照组）各80例，采用免疫组化检测两组胎盘组织中缺氧诱导因子-1ɑ及miR-34ɑ-5p的表达。结果：从GSE190639数据集中筛选出42个差异基因，上调31个，下调11个，PPI图显示与上调基因相关的基因有21个，主要富集于缺氧诱导因子-1等信号通路。GSE206763数据集中的差异miRNA有81个，上调的miRNA有miR-518b、miR-29b-3p、miR-323b-5p、miR-34ɑ-5p、miR-601等；下调的miRNA包括miR-25-3p、miR-150-3p、miR-125b-5p等，多富集于缺氧诱导因子-1等信号通路。免疫组化病例组与对照组相比，缺氧诱导因子-1ɑ及miR-34ɑ-5p均显著升高（P＜0.01）。Pearson分析发现二者呈正相关（r=0.265，P＜0.05）。结论：miR-34ɑ-5p可能通过调控HIF-1ɑ的表达参与妊娠高血压疾病的发生发展。

关键词: 妊娠高血压疾病, 潜在机制, 缺氧诱导因子-1α, miR-34a-5p

Abstract:

Objective: To explore the potential mechanism affecting the occurrence and development of hypertensive disorder complication pregnancy based on bioinformatics. Methods: Two datasets of GSE190639 and GSE206763 in GEO database were selected, and differential genes and differential miRNAs were screened by GEO2 R online platform, respectively. Volcano map, PPI map and KEGG enrichment map were drawn and analyzed accordingly. A total of 80 patients with pregnancy-induced hypertension (case group) and 80 healthy pregnant patients (control group) who were treated in the Second Affiliated Hospital of Hebei North University from January 2022 to December 2022 were selected. Immunohistochemistry was used to detect the expression of hypoxia-inducible factor-1ɑ and miR-34ɑ-5p in placental tissues of the two groups. Results: A total of 42 differential genes were screened from the GSE190639 data set, including 31 up-regulated genes and 11 down-regulated genes. PPI diagram showed that there were 21 genes related to up-regulated genes, which were mainly enriched in signal pathways such as hypoxia inducible factor-1. There were 81 differential miRNAs in the GSE206763 data set, and the up-regulated miRNAs were miR-518b, miR-29b-3p, miR-323b-5p, miR-34ɑ-5p, miR-601, etc. The down-regulated miRNAs included miR-25-3p, miR-150-3p, miR-125b-5p, etc., which were mostly enriched in signaling pathways such as hypoxia inducible factor-1. Compared with the control group, hypoxia-inducible factor-1ɑ and miR-34ɑ-5p were significantly increased in the immunohistochemical case group (P<0.01). Pearson analysis showed that there was a positive correlation between the two (r=0.265, P< 0.05). Conclusion: miR-34ɑ-5p may be involved in the occurrence and development of hypertensive disorder complication pregnancy by regulating the expression of HIF-1ɑ.

Key words: hypertensive disorder complication pregnancy, potential mechanisms , hypoxia inducible factor-1ɑ , miR-34ɑ-5p