大鼠脑缺血不同时相神经功能评分、脑梗死灶体积、血清神经元特异性烯醇化酶含量的变化

神经药理学报 ›› 2014, Vol. 4 ›› Issue (3): 17-21.

大鼠脑缺血不同时相神经功能评分、脑梗死灶体积、血清神经元特异性烯醇化酶含量的变化

郑延泽, 田彩云, 张浩楠, 何静波, 武海军, 覃建民, 徐继辉, 杨玉梅

包头医学院心血管研究室，包头，014060, 中国

出版日期:2014-06-26 发布日期:2015-01-20
通讯作者: 杨玉梅，E-mail:yym457@aliyun.com
作者简介:郑延泽，男，硕士研究生;研究方向：心脑血管与中蒙药药理研究;E-mail:491044221@qq.com
基金资助:
国家自然科学基金项目（No.81160560）

Changes of neurological score, Infarct volume , content of neuron specific enolase in serum at different phases after focal cerebral ischemia in rats

ZHENG Yan-ze, TIAN Cai-yun, ZHANG Hao-nan, HE Jing-bo, WU Hai-jun, QIN Jian-min, XU Ji-hui, YANG Yu-mei

Institute of Cardiovascular, Bao Tou Medical Collage, Baotou, 014060,China

Online:2014-06-26 Published:2015-01-20
Contact: 杨玉梅，E-mail:yym457@aliyun.com
About author:郑延泽，男，硕士研究生;研究方向：心脑血管与中蒙药药理研究;E-mail:491044221@qq.com
Supported by:
国家自然科学基金项目（No.81160560）

摘要/Abstract

摘要： 目的: 观察线栓法致大鼠局灶性脑缺血模型后，不同时相神经功能评分、脑梗死灶体积、血清神经元特异性烯醇化酶(neuron specific enolase, NSE)含量的变化。方法: 大鼠随机分为脑缺血模型组和假手术组，每组大鼠又随机分为缺血1 h组、6 h组、12 h组、24 h组、72 h组与120 h组6个时相组。采用线栓法建立大鼠局灶性脑缺血模型，观察记录大鼠大脑中动脉栓塞 ( middle cerebral artery occlusion, MCAO)后不同时相神经功能评分、测定血清NSE含量的变化,TTC染色观察脑梗死灶体积。结果:假手术组神经功能评分均为0；模型组在缺血1h神经功能评分就明显升高，24 h达到最高，持续到120 h。假手术组脑梗死灶体积均为0；模型组在缺血6h脑梗死灶体积才明显升高，24 h达到最高，72 h明显下降，120 h再次升高。假手术组血清NSE含量在各个时相没有明显变化；模型组在缺血1 h血清NSE含量就明显升高，12 h达到最高，24 h明显下降，一直持续到120 h。结论：脑缺血后神经功能评分、脑梗死灶体积、血清中NSE含量都明显升高，但三者在不同时相的变化是不一致的。

关键词: 脑缺血, 神经功能评分, 脑梗死灶体积, 神经元特异性烯醇化酶（NSE）含量

Abstract: Objective: To examine the changes of the neurological score, the infarct volume , the content of neuron specific enolase (NSE) in serum at different phases in a rat model of the focal cerebral ischemia induced by the suture method. Methods: SD rats were randomly divided into: cerebral ischemia model group and sham operation group; each group of rats was randomly divided into ischemia 1h group, 6h group, 12h group, 24h group, 72h group and 120h group of 6 phase groups. A rat focal cerebral ischemia model using the suture method was established and the changes of the neurological score, the infarct volume , the content of NSE in serum at different time phase after middle cerebral artery embolism (MCAO) of the rat were studied. Results: The sham operation group neural function score was 0, cerebral ischemia model 1h group, neural function score was significantly increased, with 24h group reaching the maxima and continuing to 120h. Sham operation group cerebral infarction index was 0, cerebral ischemia model 6h group, cerebral infarction index was significantly increased, with 24h group reaching the maxima. The 72h group was significantly reduced and 120h group was increased again. The content of NSE in serum in sham operation group was not significantly changed; cerebral ischemia model 1h group, the content of NSE in serum was significantly increased, 12h group reached the highest, 24h group was significantly reduced, and 120h group was significantly increased again. Conclusion: the neurological score, the infarct volume , the content of NSE in serum were significantly increased after cerebral ischemia. with varying phase changes.

Key words: cerebral ischemia, neurological score, infarct volume, neuron specific enolase (NSE)

中图分类号:

R965

郑延泽, 田彩云, 张浩楠, 何静波, 武海军, 覃建民, 徐继辉, 杨玉梅. 大鼠脑缺血不同时相神经功能评分、脑梗死灶体积、血清神经元特异性烯醇化酶含量的变化[J]. 神经药理学报, 2014, 4(3): 17-21.

ZHENG Yan-ze, TIAN Cai-yun, ZHANG Hao-nan, HE Jing-bo, WU Hai-jun, QIN Jian-min, XU Ji-hui, YANG Yu-mei. Changes of neurological score, Infarct volume , content of neuron specific enolase in serum at different phases after focal cerebral ischemia in rats[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(3): 17-21.

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