神经药理学报 ›› 2014, Vol. 4 ›› Issue (3): 11-16.

• 研究论文 • 上一篇    下一篇

AST对甲基苯丙胺诱导的PC-12细胞氧化损伤的保护作用

田香,茹琴,陈琳,熊琪,乐凯,马宝苗,李超英   

  1. 江汉大学武汉生物医学研究院,武汉,430056,中国
  • 出版日期:2014-06-26 发布日期:2015-01-20
  • 通讯作者: 李超英,男,教授,博士生导师;研究方向:神经电生理学;Tel:+86-027-84225417, E-mail:licy.whibs.corresp@outlook.com
  • 作者简介:田香,女,实验师;研究方向:中药活性成分的筛选和机制研究;E-mail:tianx.whibs@aliyun.com
  • 基金资助:

    国家自然科学青年基金项目(No.81302762), 国家自然科学基金面上项目(No.81473442)

Protection of Astaxanthin on the Oxidative Damage of PC-12 cells Induced by Methamphetamine

TIAN Xiang, RU Qin, CHEN Lin, XIONG Qi, YUE Kai, MA Bao-Miao, LI Chao-Ying   

  1. Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, P.R.China
  • Online:2014-06-26 Published:2015-01-20
  • Contact: 李超英,男,教授,博士生导师;研究方向:神经电生理学;Tel:+86-027-84225417, E-mail:licy.whibs.corresp@outlook.com
  • About author:田香,女,实验师;研究方向:中药活性成分的筛选和机制研究;E-mail:tianx.whibs@aliyun.com
  • Supported by:

    国家自然科学青年基金项目(No.81302762), 国家自然科学基金面上项目(No.81473442)

摘要: 目的:研究虾青素(astaxanthin,AST)对甲基苯丙胺(methamphetamine,MA)诱导的大鼠肾上腺嗜铬细胞瘤(PC-12)细胞氧化损伤的保护作用。方法:选取PC-12细胞体外培养后,随机分为正常对照组、甲基苯丙胺组、低剂量虾青素(0.1 μmol•L-1)+甲基苯丙胺组、中剂量虾青素(1 μmol•L-1)+甲基苯丙胺组、高剂量虾青素(10 μmol•L-1)+甲基苯丙胺组,通过研究PC-12细胞的存活率,以及超氧化物歧化酶(superoxide Dismutase,SOD)活性,还原性谷胱甘肽(glutathione,GSH)含量和丙二醛(malondialdehyde,MDA)含量的变化,进而评价虾青素的保护作用。结果:甲基苯丙胺能导致细胞存活率下降,同时也使SOD活性和GSH的含量降低,MDA含量增加。经过一定浓度的虾青素共处理后,细胞存活率显著提高,SOD活性和GSH含量也显著提高,MDA含量显著降低。结论:虾青素对甲基苯丙胺诱导的PC-12细胞损伤具有一定的保护作用,其机制可能与其抗氧化作用有关。

关键词: 虾青素, 甲基苯丙胺, 大鼠肾上腺嗜铬细胞瘤, 氧化损伤, 抗氧化作用

Abstract: Objective:To investigate the protective effect of astaxanthin against methamphetamine-induced oxidative damage in rat pheochromocytoma (PC-12) cells. Method: PC-12 cells were randomly divided into control group, methamphetamine group, low dose of astaxanthin (0.1 μmol•L-1)+methamphetamine group, middle dose of astaxanthin (1 μmol•L-1)+methamphetamine group and high dose of astaxanthin (10 μmol•L-1) + methamphetamine group. The protective effect of astaxanthin on the oxidative damage was observed by the cell survival rate, the activity of the superoxide dismutase (SOD), as well as the contents of glutathione (GSH) and malonidial aldehyde (MDA). Result: The results showed that astaxanthin enhanced the cell survival rate, significantly increased the activity of SOD and the content of GSH, and decreased the content of MDA. Conclusion: Astaxanthin has significant protective effect on the oxidative damage induced by methamphetamine in PC-12 cells, and the mechanism may be related to anti-oxidative function.

Key words: astaxanthin, methamphetamine, rat pheochromocytoma, oxidative damage, antioxidant

中图分类号: