神经药理学报 ›› 2022, Vol. 12 ›› Issue (3): 39-.DOI: 10.3969/j.issn.2095-1396.2022.03.008

• 2022年实验动物模型研讨会会议论文 • 上一篇    下一篇

3D回旋仪模拟微重力效应对小鼠骨骼和 心脏的影响

宋晨晨,熊毅,康台生,史旭东,高凯,董伟,郭建国,秦川   

  1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京100021,中国
  • 出版日期:2022-06-26 发布日期:2022-06-26
  • 通讯作者: 郭建国,副研究员;研究方向:环境与健康;E-mail:mybestguo@163.com 秦川,教授,博士生导师;研究方向:人类疾病动物模型创新,神经系统退行性疾病的病理学与病理生理学;E-mail:qinchuan@ pumc.edu.cn
  • 基金资助:
    中国医学科学院创新工程基金(No.2021-I2M-1-034,No.2021-I2M-1-036)

3D Clinostat Simulates the Effects of Microgravity on the Bones and Hearts of Mice

SONG Chen-chen,XIONG Yi,KANG Tai-sheng,SHI Xu-dong,GAO Kai,DONG Wei,GUO Jian-guo,QIN Chuan   

  1. Institute of laboratory Animal Science,Chinese Academy of Medical Science (CAMS),Comparative Medicine Center,Peking Union Medical College (PUMC),Beijing,100021,China
  • Online:2022-06-26 Published:2022-06-26

摘要:

目的:利用3D 回旋仪模拟微重力效应探究对小鼠骨骼和心脏的影响。方法:25 只8 周龄雄性C57BL/6J 小鼠,随机分成5 组(n=3~5):地面对照组(GC 组),3 周处理对照组(TC3 组),3 周模拟微重力组(SM3 组),6 周 处理对照组(TC6 组),6 周模拟微重力组(SM6 组)。使用Micro-CT 检测小鼠左股骨参数变化。采用超声方法检 测小鼠心脏结构和功能的变化。结果:与GC 组相比,TC3 组、SM3 组、TC6 和SM6 组小鼠的骨体积分数,骨小 梁厚度显著降低(P<0.05),骨小梁间距显著增加(P<0.05)。骨小梁数量和皮质骨壁厚度没有显著性差异。3D 回 旋仪处理3 周后,与小鼠左心室后壁厚度变薄相比,其他参数没有变化。而在第6 周,舒张期和收缩期的左心室 内径变小,左心室质量变轻。结论:3D 回旋仪模拟微重力效应会导致小鼠骨质流失,影响心脏结构和功能,且具 有时间效应。

关键词: 3D 回旋仪, 微重力, C57BL/6J 小鼠, 骨质流失, 心脏功能, 心脏结构

Abstract:

Objective:To explore the effect of 3D clinostat simulation microgravity effect on mice bones and heart. Methods:Twenty-five 8-week-old male C57BL/6J mice were randomly divided into 5 groups (n= 3~5):ground control group (GC group),3-week-treated control group (TC3 group),3 weeks simulated microgravity group (SM3 group),6-week-treated control group (TC6 group),6 weeks simulated microgravity group (SM6 group). Micro-CT was used to detect the changes of left femur parameters in mice. Results:The results showed that compared with GC group,the bone volume/tissue volume and trabecular thickness of mice in TC3,SM3,TC6 and SM6 groups were significantly decreased (P<0.05),the trabecular spaceing was significantly increased (P<0.05). There was no significant difference in trabecular number and cortical bone wall thickness. After 3 weeks of treatment with the 3D clinostat,other heart parameters did not change compared to the thinning of the LVPW in mice. Diastolic and systolic left ventricle diameter decreased,LV Mass became lighter,and tight space led to increased stroke volume and ejection fraction. Conclusion:Microgravity effects simulated by 3D clinostat can lead to bone loss in mice,and affect the structure and function of the heart with time effect.

Key words: 3D clinostat, microgravity, C57BL/6J mice, bone loss, heart function, heart structure

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