Objective: To explore the potential mechanism affecting the occurrence and development of hypertensive disorder complication pregnancy based on bioinformatics. Methods: Two datasets of GSE190639 and GSE206763 in GEO database were selected, and differential genes and differential miRNAs were screened by GEO2 R online platform, respectively. Volcano map, PPI map and KEGG enrichment map were drawn and analyzed accordingly. A total of 80 patients with pregnancy-induced hypertension (case group) and 80 healthy pregnant patients (control group) who were treated in the Second Affiliated Hospital of Hebei North University from January 2022 to December 2022 were selected. Immunohistochemistry was used to detect the expression of hypoxia-inducible factor-1ɑ and miR-34ɑ-5p in placental tissues of the two groups. Results: A total of 42 differential genes were screened from the GSE190639 data set, including 31 up-regulated genes and 11 down-regulated genes. PPI diagram showed that there were 21 genes related to up-regulated genes, which were mainly enriched in signal pathways such as hypoxia inducible factor-1. There were 81 differential miRNAs in the GSE206763 data set, and the up-regulated miRNAs were miR-518b, miR-29b-3p, miR-323b-5p, miR-34ɑ-5p, miR-601, etc. The down-regulated miRNAs included miR-25-3p, miR-150-3p, miR-125b-5p, etc., which were mostly enriched in signaling pathways such as hypoxia inducible factor-1. Compared with the control group, hypoxia-inducible factor-1ɑ and miR-34ɑ-5p were significantly increased in the immunohistochemical case group (P<0.01). Pearson analysis showed that there was a positive correlation between the two (r=0.265, P< 0.05). Conclusion: miR-34ɑ-5p may be involved in the occurrence and development of hypertensive disorder complication pregnancy by regulating the expression of HIF-1ɑ.