ACTA NEUROPHARMACOLOGICA

ACTA NEUROPHARMACOLOGICA ›› 2021, Vol. 11 ›› Issue (2): 5-10.DOI: 10.3969/j.issn.2095-1396.2021.02.002

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Differentiation of CD4+CD25- T Cells to Regulatory T Cells induced by Diosgenin in CIA Mice

LIANG Xiu-jun，YU Yong-zhou，XIA Dong-shuai，GAO Ya-xian，SUN Tong-you   

  1. 1 Chengde Medical University，Chengde，067000，China

    2 Hebei Key Laboratory of Nerve Injury and Repair，Chengde 067000，China

    3 Chengde Central Hospital，Chengde，067000，China

  • Online:2021-04-26 Published:2021-04-26
  • About author:梁秀军，女，讲师；研究方向：中药免疫；E-mail：149304426@qq.com
  • Supported by:
    河北省高等学校科学技术研究项目（No.QN2016031），河北省中医药管理局科研计划项目（No.2015155），河北省科技厅中央引导地方科技发展基金项目（No.216Z2501G）

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Abstract:

Objective：To investigate the roles of diosgenin in the differentiation of regulatory T cells from CD4+CD25-T cells in collagen induced arthritis mice. Methods：

CD4+CD25-T cells of CIA mice isolated by immunomagnetic beads on magnetic activated cell sorting device were co-cultured with different concentrations of diosgenin（25、12.5、6.25 μmol·L-1） for 48 hours. The ratio of Treg cells was tested by flow cytometry. The level of Foxp3 mRNA was measured by Real-Time PCR. The level of serum IL-10 was detected by ELISA. Results：This study separated CD4+CD25-T cells of 93.89% purity. The ratio of Treg cells，the level of IL-10 and Foxp3 mRNA were increased significantly in diosgenin groups compared with those in control group（P<0.05）. Conclusion：Diosgenin may induce the differentiation of CD4+CD25-T cells to regulatory T cells in CIA mice and up-regulate the expression of Foxp3.

Key words:

font-family:Times, "> CIA modelfont-family:Helvetica, ">；diosgeninfont-family:Helvetica, ">；regulatory T cells

CLC Number: