Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury

ACTA NEUROPHARMACOLOGICA ›› 2014, Vol. 4 ›› Issue (6): 1-5.

Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury

WEN Zhi-ling, WANG Zhen-zhen, HE Wen-bin, CHEN Nai-hong

Online:2014-12-26 Published:2015-01-20
Contact: 贺文彬，男，博士，研究生导师；E-mail:hewb@sxtcm.com 陈乃宏，男，，研究员，博士生导师；研究方向：分子神经药理学、中药药理学。E-mai:chennh@imm.ac.cn
About author:温智林，男，硕士研究生；研究方向:中医药防治抑郁症的基础研究。E-mai:627297979@qq.com
Supported by:
本研究由国家自然科学基金项目（No. 81274122、No. 81202507、No. 81273629），教育部博士点基金重点项目（No. 20121106130001），新药作用机制研究与药效评价北京市重点实验室资助项目（No. BZ0150），中央级公益性科研院所基本科研业务费，专项资金（No. 2014RC03），山西省回国留学人员科研资助项目（2013-134）、山西省国际科技合作项目（2010081065）资助。

Abstract

Abstract: Objective: To observe the effects of Kaixin powder containing serum on corticosterone induced injury of rat cortical astrocyte CTX TNA2 cell line. Methods: Firstly, SD rats were orally administered with Kaixin powder, positive drug fluoxetine (10 mg·kg-1) and solvent control in order to obtain corresponding serum. CTX TNA2 cell line injured by corticosterone was used to establish depression model in vitro and cell activity was detected with MTT. Then we tested whether Kaixin powder play a neuroprotective role through PI3K signaling pathway in this cell model with the inhibitors of this pathway and detected by western blotting analysis. Results: According to our data, when the corticosterone concentration reached 200 μM, the cell viability inhibition rate was stabilized at 60% with a well concentration dependent. Kaixin powder containing serum could significantly improve the survival rate of corticosterone injured CTX TNA2 cells. The western blotting results also showed that Kaixin powder containing serum could reverse the reduction of PI3K and AKT1/2 protein expression induced by corticosterone. However, after given the inhibitors of PI3K pathway, the protective effect of Kaixin powder was reversed. Conclusion: Kaixin powder containing serum has obvious protective effect on corticosterone injured CTX TNA2 cell line, and the mechanism may be associated with nerve cell protective related PI3K signal transduction pathway.

Key words: Kaixin powder, containing serum, corticosterone, PI3K, CTX TNA2

CLC Number:

R965

WEN Zhi-ling, WANG Zhen-zhen, HE Wen-bin, CHEN Nai-hong. Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(6): 1-5.

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