Antioxidant effects of Orientin and Vitexin in Trollius Chinesis in D-galactose-induced mice model of aging

ACTA NEUROPHARMACOLOGICA ›› 2012, Vol. 2 ›› Issue (3): 1-6.

Antioxidant effects of Orientin and Vitexin in Trollius Chinesis in D-galactose-induced mice model of aging

TIAN Qing-qing, WANG Shu-hua, AN Fang

Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China

Online:2012-06-26 Published:2013-12-25
Contact: 安芳,男,教授,硕士生导师；研究方向:中药有效成分提取及其药理活性研究；Tel:+86-0313-4029558 ，E-mail：anfanghbnu@126.com
About author:田青青，女，2012级研究生；研究方向：中药有效成分提取及其药理活性研究；E-mail：tianqingqing900927@126.com.cn
Supported by:
张家口市科技局资助项目（No.11110015D),河北北方学院自然基金（No.2011）

Abstract

Abstract: Objective: To study the antioxidant effects of orientin and vitexin from Trollius chinensis Bunge. Methods: The aged mouse model was established through Daily intraperitoneal injection of D-galactose for 8 weeks was used to establish experimental aging in mice. Different groups of mice were treated with 40, 20 or 10 mg·kg^-1 orientin, vitexin, or a positive control (vitamin E) via intragastric administration for an additional 8 weeks. The total antioxidant capacity in the serum, activities of the antioxidant enzyme and the cell membrane transport ion ATP enzyme, and the level of malondialdehyde in the liver, brain and kidneys were determined. Results: Orientin, vitexin, and vitamin E produced a significant increase in total antioxidant capacity, in the serum, and the levels of superoxide dismutase, catalase and glutathione peroxidase, Na⁺-K⁺-ATP enzyme, and Ca²⁺-Mg²⁺-ATP enzyme in the liver, brain and kidneys. In addition, they significantly reduced the malondialdehyde levels in the liver, brain and kidney. The 40 mg·kg^-1dose of orientin and vitexin had similar antioxidant effects as vitamin E. Conclusion: Orientin and vitexin have anti-aging effects possibly through their antioxidant activities.

Key words: orientin, vitexin, D-galactose, antioxidation

CLC Number:

R965

TIAN Qing-qing, WANG Shu-hua, AN Fang. Antioxidant effects of Orientin and Vitexin in Trollius Chinesis in D-galactose-induced mice model of aging[J]. ACTA NEUROPHARMACOLOGICA, 2012, 2(3): 1-6.

References

[1] 苏连杰, 田鹤, 马丽英. 金莲花醇提物体内抗病毒作用的实验研究[J]．中草药, 2007, 38(7)：1062-1064．

[2] Li Yao-Lan, Ma Shuang-Cheng, Yang Yi-Ting, et al．Antiviral activities of flavonoids and origanic acid from Trollius chinensis Bunge [J]．J Ethnophopharmacol, 2002, 79(3)：365-368．

[3] 朱登祥, 安芳, 王书华. 金莲花中牡荆苷对人食管癌EC-109细胞生长及凋亡的影响[J].中草药, 2012, 43(9)：1781-178.

[4] 蒋伟, 屈海琪, 王书华, 等. 金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脑损伤的保护作用研究[J]. 中草药, 2012, 43(7)：1376-1380.

[5] 王书华, 杨国栋, 饶娜, 等. 金莲花中荭草苷和牡荆苷对D-半乳糖致衰小鼠体内血清抗氧化作用的影响[J]. 中国老年学杂志, 2011, 31(24)：4818-4820.

[6] 饶娜, 杨国栋, 安芳, 等. 金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脂褐素和脑组织形态学的影响[J].中国老年学杂志, 2012, 32(3)：562- 564.

[7] Cui X, Zou P, Zhang Q, et al．Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice protective effects of R-alpha-lipoic acid[J]．Neurosci Res, 2006, 83(8): 1584-1590．

[8] 刘洋, 程清州, 彭超华, 等. D-半乳糖致小鼠衰老模型的制作及效果评价[J].武汉工业学院学报,2009, 28(1)：32-36.

[9] 李云, 杨素青, 张军．急性致衰老动物模型的探讨[J], 卫生研究, 2002, 4：290-291．

[10] Sukhjit Kaur Sandhu, Gurcharan Kaur. Alterations in oxidative stress scavenger system in aging rat brain and lymphocytes[J]. Biogerontology, 2002, 3(3):161-173.

[11] Yang Guo-yuan, Chen S F, Kinouehi H, et al. Edema cation content and ATPase activity after middle cerebral altery occlusion in rats[J] Stroke, 2007, 23(9):1331-1336.

[12]张伟, 张艳淑, 姚林, 等. Oncolyn对衰老小鼠脑组织中ATP酶活性和神经生长因子的表达的影响[J]. 卫生研究, 2007, 36(2)：164-166.

[13]王新玲, 李丹彩, 侯颖春. 自由基、细胞凋亡与衰老关系的研究进展[J].中国老年学杂志, 1999, 19(4):252-253.

[14]Germann I, Hagelauer D, Kelber O, et al. Antioxidative properties of the gastrointestinal phytopharmaceutical remedy STW 5 (Iberogast)[J]. Phytomedicine, 2006, 13(Suppl 5): 45-50.

[15]Wu X A, Qing F, Du M Q. The QSAR study on anti-inflammatory activities of C-glycosy flavones[J]. Shizhen Guoyi Guoyao, 2012, 23(3)：632-633.