Abstract: Objective：To investigate the effects of Phillyrin on 1-methyl-4-phenylpyridinium (MPP⁺) -induced injury in SH-SY5Y neuroblastoma cells. Methods：SH-SY5Y cells were incubated with Phillyrin of different concentrations. MTT metabolic rate was measured as the cell survival rate to determine the effective concentrations of Phillyrin. After SH-SY5Y cells were incubated with Phillyrin (1，10，100 μmol·L^-1) for 24 h prior to exposure to MPP⁺ (1 mmol·L^-1) for 48 h，the MTT metabolic rate and LDH leakage rate were observed. Results：Phillyrin did not decrease the survival rate of SH-SY5Y cells at the concentrations from 1 to 100 μmol·L^-1. Compared with the control group，MPP⁺ decreased the MTT metabolic rate （P<0.001） and increased the LDH leakage rate (P<0.001) of SH-SY5Y cells. However，Phillyrin significantly increased the cell activity (P<0.05) and decreased LDH leakage rate of SH-SY5Y cells with MPP⁺-induced injury. Conclusion：Phillyrin protects SH-SY5Y cells against MPP⁺-induced cytotoxicity.