Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury

Abstract

Abstract: Objective: To observe the influence of chrysophanol liposomes on apoptosis of hippocampal neurons in mice and to study the protective effects on cerebral ischemia and reperfusion injury and its mechanism. Methods: Cerebral ischemia and reperfusion injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. Chrysophanol liposomes（10.0，1.0，0.1 mg· kg^-1）was injected intraperitoneally. The effects of chrysophanol liposomes were studied on the neurological function, the pathomorphology of brain tissues and the expression of apoptosisrelated protein caspase-3, Bcl-2 and Bax in hippocampal neuron using immunohistochemistry. Results： Chrysophanol liposomes could increase the expression of Bcl-2, decrease the expression of Bax and caspase-3, improve the neurological function score and decrease the pathological change in chrysophanol liposomes treated animals. The effect of chrysophanol liposomes was most obviously in group 10.0 mg· kg^-1（P<0.05）. Conclusion: Chrysophanol liposomes have a significant protective effect on hippocampal neurons after cerebral ischemia and reperfusion injury. The mechanism may be related to increasing the expression of Bcl-2, reducing caspase-3 and Bax.

Key words: chrysophanol liposomes, cerebral ischemia and reperfusion injury, hippocampal, apoptosis, caspase-3, Bcl-2, Bax

CLC Number:

R971

SONG Jin-yan, ZHANG Li, ZHAO Xiao-qian, SONG Zhi-bin. Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(2): 7-13.

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Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury

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