Cultivation and identification of neural stem cells cultivated by the methods of neurospheres and adherent monolayer cells

Abstract

Abstract: Objective:AIM To explore two methods of culturing mouse embryo neural stem cells in vitro and identification of their differentiation． Methods: The neural stem cells were derived from the cortex tissue of mouse embryo at gestation day of 14～16 with two methods: culturing of neurosphere in floating and culturing of monolayer adherent cells. To observe the development of the neural stem cell，the expression of the stem cell marker Nestin and the neural marker MAP-2 and the glial marker GFAP was examined with immunofluorescence. Results: The cultured neural stem cells proliferated．The maintenance of stem cell property was preserved with both methods as revealed by Nestin immunofluorescence. These stem cells are able to differentiate into glial (GFAP positive) and neuronal lineage (MAP-2positive). Conclusion: With the help of growth factors and serum-free technique，the mouse neural stem cells cultivated by methods of neural stem cell spheres and adherent monolayer cells，have potential of proliferation and differentiation.

Key words: cerebral cortex, neural stem cells, neurosphere, monolayer adherent cells

HAO Jun-Rong, ZHANG Li, QIN Zheng-Hong. Cultivation and identification of neural stem cells cultivated by the methods of neurospheres and adherent monolayer cells[J]. Journal of Materials Sciences & Technology, 2011, 1(1): 51-55.

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