神经药理学报

神经药理学报 ›› 2015, Vol. 5 ›› Issue (4): 22-25.

• 实验方法学 • 上一篇    下一篇

采用Ⅶ型胶原酶制作大鼠脑出血模型的技巧

李东方，赵海滨，姜天元，翟瑶瑶，杨丹丹   

  1. 北京中医药大学第三附属医院，北京，100029，中国
  • 出版日期:2015-08-26 发布日期:2016-03-03
  • 通讯作者: 赵海滨，男，医学博士后，教授，博士生导师；研究方向：中医药防治心脑血管疾病； Tel：+86-010-52075251，E-mail：haibin999@126.com
  • 基金资助:

    国家自然基金项目（No.81373590）

The Skills of Intracerebral Hemorrhage Rat Model by Type VII Collagenase Injection

LI Dong-fang, ZHAO Hai-bin, JIANG Tian-yuan, ZHAI Yao-yao, YANG Dan-dan   

  1. The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine, Beijing, 10029, China
  • Online:2015-08-26 Published:2016-03-03
  • Contact: 赵海滨，男，医学博士后，教授，博士生导师；研究方向：中医药防治心脑血管疾病； Tel：+86-010-52075251，E-mail：haibin999@126.com
  • Supported by:

    国家自然基金项目（No.81373590）

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摘要： 目的：研究Ⅶ型胶原酶脑内注入法构建大鼠脑出血模型的制作技巧。方法：运用立体定向技术及本文改进了的方法，向大鼠尾状核内缓慢注入Ⅶ型胶原酶，制作大鼠脑出血模型，通过观察其行为学变化，初步评价该模型建立的可行性和稳定性。结果：对150只SD大鼠进行脑出血造模，2只死亡，9例不成功，成功率为92.7％。结论：Ⅶ型胶原酶注射可建立理想稳定的脑出血模型，且操作简单，有较大推广价值。

关键词: 胶原酶, 大鼠, 脑出血, 模型

Abstract: Objective: To study the skills of intracerebral hemorrhage model by injecting type Ⅶ collagenase into brain region in rats. Methods: The animal model of intracerebral hemorrhage was induced by the injection of type Ⅶ collagenase into caudate nucleus of rats with stereotactic instrument and improved methods. And then evaluate the feasibility and stability of the model through behavioral assessment initially. Results: we had made 150 rat models, two rats died, nine rats was not successful. The success rate of intracerebral hemorrhage model was 92.7%. Conclusion: The method of injecting type Ⅶ collagenase can be used to build the animal model of intracerebral hemorrhage ideally and steadily. Because of the simple experiment operation, it has a great promotional vaule.

Key words: collagenase, rat, intracerebral hemorrhage, model