硝酸甘油诱导的偏头痛大鼠自主活动的变化

神经药理学报 ›› 2015, Vol. 5 ›› Issue (3): 6-11.

硝酸甘油诱导的偏头痛大鼠自主活动的变化

王克柱，孙军花，徐攀，陈红英，代志，王晓英，刘新民

1．药用植物研究所药理毒理中心，北京协和医学院，中国医学科学院，北京，100193，中国
2．华润三九医药股份有限公司，深圳，518110，中国

出版日期:2015-06-26 发布日期:2015-07-06
通讯作者: 刘新民，男，博士生导师；研究方向：神经药理学；E-mail：liuxinmin@hotmail.com
作者简介:王克柱，男，北京协和医学院在读博士生；研究方向：神经药理学；E-mail：wangkezhu0628@163.com
基金资助:
科技部重大新药创制（2013ZX09201022）

Migraine Model Rats Evoked by Nitroglycerin Decrease Autonomic Activity

WANG Ke-zhu, SUN Jun-hua, XU pan, CHEN Hong-ying, DAI Zhi, WANG Xiao-ying, LIU Xin-min

1. Research Center for Pharmacology ＆ Toxicology, Institute of Medicinal Plant Development ( IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
2. China Resources Sanjiu Medical &Pharmaceutical Co.,Ltd. Shenzhen, 518110, China

Online:2015-06-26 Published:2015-07-06
Contact: 刘新民，男，博士生导师；研究方向：神经药理学；E-mail：liuxinmin@hotmail.com
About author:王克柱，男，北京协和医学院在读博士生；研究方向：神经药理学；E-mail：wangkezhu0628@163.com
Supported by:
科技部重大新药创制（2013ZX09201022）

摘要/Abstract

摘要： 目的：皮下注射硝酸甘油复制大鼠偏头痛模型，并研究偏头痛大鼠运动能力和情绪的变化。方法：皮下注射硝酸甘油构建大鼠偏头痛模型，应用空场实时监测系统实时记录模型大鼠造模后10、30、60、90 min的自主运动情况。结果：皮下注射硝酸甘油后大鼠前肢挠头、后肢挠头、甩头次数增多，且在造模后30 min内偏头痛行为学症状最明显，随时间的增加大鼠逐渐恢复正常。与对照组比较，偏头痛大鼠的短时运动（10、30 min）无明显变化，但中央区运动路程减少，周边区运动路程增加，说明偏头痛大鼠表现出焦虑情绪。观测偏头痛大鼠长时运动（90 min）情况，与对照组比较，自60 min后模型大鼠的运动路程、运动时间显著性降低，且随着时间的增加，运动路程也逐渐的减少。结论：皮下注射硝酸甘油引起大鼠搏动性头痛，挠头、甩头等行为学症状增多，同时影响了大鼠的自主活动能力，并使动物产生焦虑情绪。

关键词: 硝酸甘油, 偏头痛, 运动能力, 焦虑

Abstract: Objective：Migraine mode of rats was made by subcutaneous injection（s.c）of nitroglycerin and the change of antonomic activity was researched. Methods：The migraine rat model was constructed by subcutaneous injection of nitroglycerin，and autonomic activities in 10，30，60，90 min after s.c nitroglycerin were real-time recorded by open stopping system. Results：Compared with control group，the special behaviors and symptoms，such as head scratching and shaking，increased in the migraine rats，and was most obvious within 30 min. The short movements（10，30 min） of migraine rats were not significantly changed. The movement distance in central area of model rats was significantly decreased，and the movement distance of peripheral area signifi cantly increased compared with control rats. These indicated that the s.c nitroglycerin rats exhibited anxiety symptom. Compared with control group，the movement distance and time of model group were decreased after 60 min，and with the increase in time，movement distance also gradually reduced. Conclusion：The increase of special behaviors and symptoms，such as the scratching head and shaking in migraine rats could be caused by s.c nitroglycerin，and meanwhile the model rats exhibited anxiety and impaired the autonomic activities were impaired.

Key words: nitroglycerin, migraine, motor ability, anxiety

中图分类号:

R965

王克柱，孙军花，徐攀，陈红英，代志，王晓英，刘新民. 硝酸甘油诱导的偏头痛大鼠自主活动的变化[J]. 神经药理学报, 2015, 5(3): 6-11.

WANG Ke-zhu, SUN Jun-hua, XU pan, CHEN Hong-ying, DAI Zhi, WANG Xiao-ying, LIU Xin-min. Migraine Model Rats Evoked by Nitroglycerin Decrease Autonomic Activity[J]. Acta Neuropharmacologica, 2015, 5(3): 6-11.

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