神经药理学报 ›› 2015, Vol. 5 ›› Issue (3): 1-5.

• 研究论文 •    下一篇

丙泊酚对老年大鼠认知功能及大脑海马CA1 区NMDAR2B表达的影响

乔霖,赵薇,王新生,邢珍   

  1. 河北北方学院,张家口,075000,中国
  • 出版日期:2015-06-26 发布日期:2015-07-06
  • 通讯作者: 邢珍,女,医学硕士,教授,硕士研究生导师;研究方向:麻醉药物与POCD
  • 作者简介:乔霖,男,硕士研究生;研究方向:静脉麻醉药与POCD
  • 基金资助:

    河北北方学院自然科学研究计划项目(重大项目:120176)

Effect of Propofol on the Cognitive Function and NMDAR2B Expression of CA1 in the Hippocampus of Aged Rats

QIAO Lin, ZHAO Wei, WANG Xin-sheng, XING Zhen   

  1. HeBei North University, Zhangjiakou, 075000, China
  • Online:2015-06-26 Published:2015-07-06
  • Contact: 邢珍,女,医学硕士,教授,硕士研究生导师;研究方向:麻醉药物与POCD
  • About author:乔霖,男,硕士研究生;研究方向:静脉麻醉药与POCD
  • Supported by:

    河北北方学院自然科学研究计划项目(重大项目:120176)

摘要: 目的:通过观察丙泊酚麻醉对老年大鼠学习记忆影响以及海马CA1区NMDAR2B表达的变化,探讨丙泊酚在术后认知功能障碍(postoperative cognitive dysfunction,POCD)中的作用。方法:雄性SD老年大鼠随机分为对照组、丙泊酚组,每组20只。丙泊酚组给予50 mg·kg-1丙泊酚腹腔麻醉,对照组给予相同剂量的乳剂,麻醉后1d进行Morris水迷宫测试,结束后采集大鼠海马,采用免疫荧光和FISH技术检测海马CA1区NMDAR2B蛋白表达及基因转录的变化。结果: Morris水迷宫结果:丙泊酚组与对照组相比潜伏时间明显延长,穿越次数明显减少。免疫荧光结果:丙泊酚组与对照组比较NMDAR2B表达明显下降。FISH结果:丙泊酚组与对照组比较NMDAR2B表达明显下降。结论:丙泊酚麻醉老年大鼠短期认知功能障碍,其机制可能与海马CA1区NMDAR2B表达下调有关。

关键词: 丙泊酚, 术后认知障碍, NMDAR2B

Abstract: Objective:The effect of propofol anesthesia on learning memory and NMDAR2B expression of CA1 in hippocampus were observed and the effect of propofol on postoperative cognitive dysfunction( POCD) was discussed. Methods:Male SD aged rats were randomly divided into control group and propofol group,with 20 rats in each group. Propofol group was given 50 mg·kg-1 propofol by intraperitoneal injection. The control group was given the same dose of emulsion. The Morris water maze test was performed on 24 h after the end of anesthesia. The expression of NMDAR2B protein and gene transcription of CA1 region were detected by immunofluorescence and FISH. Results:In Morris water maze test:compared with the control group,the latent period was signifi cantly prolonged,and the crossing times of propofol group were signifi cantly decreased. In immunofluorescence detection,the expression of NMDAR2B was significantly decreased in propofol group. In FISH,compared with the control group,the expression of NMDAR2B decreased significantly. Conclusion:Propofol anesthesia can induce a short-term cognitive dysfunction in aged rats,and its mechanism may be related to the down-regulation of NMDAR2B expression in hippocampus CA1 region.

Key words: Propofo, POCD, NMDA2B receptor

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