原花青素联用胡椒碱减轻抑郁样行为的机制研究

神经药理学报 ›› 2014, Vol. 4 ›› Issue (2): 8-15.

原花青素联用胡椒碱减轻抑郁样行为的机制研究

胡慧, 潘博士, 黄梦琦, 毕梦云, 潘建春

温州医科大学药学院，温州，325035，中国

出版日期:2014-04-26 发布日期:2014-06-27
通讯作者: 潘建春，男，教授，硕导，研究方向：抑郁症，阿尔茨海默症，E-mail：wenzhoupan2003@163.com，传真：0577-86689983，Tel：0577-86689982
作者简介:胡慧，女，本科。主要从事精神病学和精神药理学研究，E-mail：386637016@qq.com
基金资助:
温州医科大学校级课题（编号：wyx201301051）

Amelioration of Depression-like Behaviors with Proanthocyanidins in Combination with Piperine

HUI Hu, PAN Bo-shi, HUANG Qi-meng, BI Meng-yun, PAN Jian-chun

School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035, China

Online:2014-04-26 Published:2014-06-27
Contact: 潘建春，男，教授，硕导，研究方向：抑郁症，阿尔茨海默症，E-mail：wenzhoupan2003@163.com，传真：0577-86689983，Tel：0577-86689982
About author:胡慧，女，本科。主要从事精神病学和精神药理学研究，E-mail：386637016@qq.com
Supported by:
温州医科大学校级课题（编号：wyx201301051）

摘要/Abstract

摘要： 目的：探讨原花青素（proanthocyanidin，OPC）不同剂量联用胡椒碱（piperine，PIP）的抗抑郁样行为及作用机制。方法：采用慢性不可预知性温和应激模型，连续造模联合给药21 d后，第22 d测定小鼠悬尾、强迫游泳不动时间；用高效液相电化学法测定小鼠海马和前额叶皮层中单胺递质及其代谢产物的含量，荧光分光光度法检测全脑单胺氧化酶活性。结果：与正常组相比，模型组小鼠悬尾和强迫游泳测试中的不动时间显著增加；同时其海马、前额叶皮层中5-羟色胺（5-hydroxytryptamine，5-HT）、去甲肾上腺素（Norepinephrine，NE）含量显著下降，但5-羟吲哚乙酸(5-hydroxyindoleacetic acid, 5-HIAA)与5-HT比值（5-HIAA/5-HT）显著上升。OPC (50，25，12.5 mg·kg-1)剂量组联用PIP（5 mg·kg-1）能够逆转这种现象。此外，OPC (50，25，12.5 mg·kg-1) 剂量组联用胡椒碱（5 mg·kg-1）组还能降低由于应激引起的单胺氧化酶（monoamine oxidase, MAO）A型活性升高，但不影响单胺氧化酶（monoamine oxidase, MAO）B型活性。结论：原花青素联用胡椒碱可以改善由于慢性应激对小鼠造成的抑郁样行为，其机制可能与降低单胺氧化酶活性，增加单胺递质含量有关。

关键词: 原花青素, 抑郁, 胡椒碱, 联用

Abstract: Objective: To investigate the effects of different doses (50，25，12.5 mg·kg-1) of Proanthocyanidin (OPC) combined with piperine (5 mg·kg-1) on depression-like behaviors in mice under chronic unpredictable mild stress. Methods: Chronic stress was applied for 21 days. After that, the immobility time of mice in the forced swimming test and tail suspension test were observed respectively. In addition, the HPLC-ECD was used to analyze the concentration of monoamines and their metabolites in the hippocampus and frontal cortex, and Fluorescence spectrophotometry was used to analyze the monoamine oxidase activity in mice brain. Results: Compared with the control group，the stressed group decreased the immobility time in the forced swimming test and tail suspension test ; At the same time, reduced 5-HT and NE levels, but increased the ratio of 5-HIAA/5-HT in the hippocampus and frontal cortex of mice were observed. The OPC (50，25，12.5 mg·kg-1) combined with piperine (5 mg·kg-1) showed a significantly larger effect on these measures. In addition, MAO-A activity in the brain was increased significantly when the mice were exposed to chronic stress, but MAO-B activity was not changed. The combination strategy could significantly decrease MAO-A activity which was raised by chronic stress, while they did not affect the MAO-B activity. Conclusion: Proanthocyanidins in combination with piperine ameliorated depression-like behaviors induced by chronic unpredictable mild stress in mice, which may be partially mediated through the serotonin and noradrenaline systems.

Key words: proanthocyanidins, depression, piperine, combination

中图分类号:

R965

胡慧, 潘博士, 黄梦琦, 毕梦云, 潘建春. 原花青素联用胡椒碱减轻抑郁样行为的机制研究[J]. 神经药理学报, 2014, 4(2): 8-15.

HUI Hu, PAN Bo-shi, HUANG Qi-meng, BI Meng-yun, PAN Jian-chun. Amelioration of Depression-like Behaviors with Proanthocyanidins in Combination with Piperine[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(2): 8-15.

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